Additional file 1: of Clinical and genetic characteristics of Chinese patients with familial or sporadic pediatric cataract

Table S1. List of 80 pediatric cataract genes. (XLS 36 kb

Additional file 3: of Clinical and genetic characteristics of Chinese patients with familial or sporadic pediatric cataract

Table S3. Target NGS analysis. (XLS 28 kb

Relative expression of Oct4 and Nanog genes in porcine SCNT embryos at Blastoyst stages.

<p>Values with different superscript letters (a, b) within groups indicate significant difference (<i>p</i> < 0.05).</p

The expression pattern of miR-148a in embryos of SCNT, PA and IVF.

<p>Values with different superscript letters (a, b) within groups indicate significant difference (<i>p</i> < 0.05).</p

Expression patterns of 5-mC and H3K9ac in porcine Con-NT embryos, miR-148a-NT embryos, and miR-con-NT embryos.

<p>Note: A-C, J-L: Con-NT; D-F, M-O: miR-148a-NT; G-I, P-R: miR-Con-NT; A-I: 5-mC, J-R: H3K9ac; Red: 5-mC or H5K9ac; Blue: DNA; 5-mC and H3K9ac (capital letter); DNA (lowercase letter). Scare bar = 50μm.</p

Effect of miR-148a on DNA methylation in PFFs.

<p>(A) Methylation patterns were determination on the basis of the relative levels of 5-methyl-cytosine (5-mC) in PFFs. Each sample was counter-stained with DAPI to visualize DNA. (B) Average optical intensity of global DNA methylation measured using Image-Pro Plus 6.0 software. Values are presented as mean ± SEM. Values with different superscript letters (a, b) within groups indicate significant difference (<i>p</i> < 0.05). Scare bar = 100μm.</p

MicroRNA-148a overexpression improves the early development of porcine somatic cell nuclear transfer embryos - Fig 6

<p><b>Relative levels of 5-mC (A), H3K9ac (B) in porcine SCNT embryos.</b> Values with different superscript letters (a, b) within groups indicate significant difference (<i>p</i> < 0.05).</p

miR-148a inhibited DNMT1expression.

<p>(A) miR-148a expression was increased in PFFs of overexpressing miRNA-148a. (B) DNMT1 expression was decreased in PFFs of overexpressing miRNA-148a. DNMT3a expression has no difference in PFFs of overexpressing miRNA-148a compared with control group. (C) Western blot assays confirmed that DNMT1 expression were downregulated by in PFFs of over-expression miR-148a. Values with different superscript letters (a, b) within groups indicate significant difference (<i>p</i> < 0.05).</p

Effect of miR-148a on the development of porcine SCNT embryos.

<p>Effect of miR-148a on the development of porcine SCNT embryos.</p

DNMT1 was target gene of miR-148a.

<p>(A) miR-148a is highly complementary to pig DNMT1 3'UTR. This potential miR-148a target site is conserved in human, cows, mice and pigs. (B) Schema contains WT and Mut 3-′UTRs of DNMT1, indicating the interaction sites between miR-148a and 3-′UTR of DNMT1. (C) Schema of miR-148a precursor sequence in pEZX-MR03. (D) Luciferase activities were measured in 293T cells co-transfected with the pEZX-DNMT1 (wild type or mutant) and pEZX-miR-148a or pEZX-miR-control. The data were reported as mean ± S.D. for three independent experiments (<i>p</i> < 0.05).</p