Graphic showing the correlation between positive cultures and PCR Cq values.

<p>Comparison of PCR and culture results for <i>E</i>. <i>coli</i>. Cq values of PCR are depicted in the blue bars ranging from Cq22-Cq40 on the left axis. The red bars depict positive cultures with growth of 10³, 10⁵, and >10⁵ cfu/ml on the right axis. Low Cq values correspond with high loads of micro-organisms, and thus with larger yield of positivity and quantities of cultures.</p

Amplification of serial dilutions of bacterial cells.

<p>Standard curves (top) of PCRs for detection of <i>Citrobacter/Enterobacter spp</i>. (yellow), <i>Klebsiella spp</i>. (blue), <i>E</i>.<i>coli</i> (red), <i>E</i>.<i>faecalis</i> (brown), <i>P</i>.<i>mirabilis</i> (dark blue), and <i>P</i>.<i>aeruginosa</i> (purple). The Cq values per dilution are shown below the figure. Cq values that correspond with 10³ and 10⁵ cfu/ml were underlined in blue and red, respectively.</p

Analysis of results of PCR and the Seegene kit.

<p>Analysis of results of PCR and the Seegene kit.</p

Analysis of results of PCR and culture.

<p>Analysis of results of PCR and culture.</p

PCR positive urine specimens with inconclusive culture results (n = 18).

<p>PCR positive urine specimens with inconclusive culture results (n = 18).</p

PCR positive and culture positive urine specimens (n = 44).

<p>PCR positive and culture positive urine specimens (n = 44).</p