Noninvasive prediction of Blood Lactate through a machine learning-based approach.

We hypothesized that blood lactate concentration([Lac]blood) is a function of cardiopulmonary variables, exercise intensity and some anthropometric elements during aerobic exercise. This investigation aimed to establish a mathematical model to estimate [Lac]blood noninvasively during constant work rate (CWR) exercise of various intensities. 31 healthy participants were recruited and each underwent 4 cardiopulmonary exercise tests: one incremental and three CWR tests (low: 35% of peak work rate for 15 min, moderate: 60% 10 min and high: 90% 4 min). At the end of each CWR test, venous blood was sampled to determine [Lac]blood. 31 trios of CWR tests were employed to construct the mathematical model, which utilized exponential regression combined with Taylor expansion. Good fitting was achieved when the conditions of low and moderate intensity were put in one model; high-intensity in another. Standard deviation of fitting error in the former condition is 0.52; in the latter is 1.82 mmol/liter. Weighting analysis demonstrated that, besides heart rate, respiratory variables are required in the estimation of [Lac]blood in the model of low/moderate intensity. In conclusion, by measuring noninvasive cardio-respiratory parameters, [Lac]blood during CWR exercise can be determined with good accuracy. This should have application in endurance training and future exercise industry

Modeling the effects of satiation on the feeding rate of a colonial suspension feeder, Acanthogorgia vegae, in a circulating system under lab conditions

(2002) Modeling the effects of satiation on the feeding rate of a colonial suspension feeder, Acanthogorgia vegae, in a circulating system under lab conditions. Zoological Studies 41(4): 355-365. We developed a mathematical model, based on observations relating to feeding behavior of the gorgonian Acanthogorgia vegae, to characterize the feeding rates of colonial suspension feeders in a circulating system. We found that simultaneously none of all the polyps belonging to the same colony of A. vegae showed a feeding response after capturing a certain amount of Artemia nauplii. Equations in terms of the satiation factor were derived to predict feeding rates of colonial suspension feeders. Flume experiments were conducted under different ambient flow regimes to test the application of the equations. Good agreement between the results of the experiments and the predictions was evident. The results of this study show that satiation is an important factor influencing the feeding rate of colonial suspension feeders with an abundant food supply and a closed system of water. Furthermore, our study shows that the feeding rate of colonial suspension feeders in a circulating system can easily be calculated from the residual number of prey items at various times, even when the initial numbers of prey items and feeding animals are unknown. Our model is useful as a good methodological tool to estimate the feeding rate of colonial suspension feeders in the laboratory or aquacultural environments

Discovery of gamma-ray emission from a strongly lobe-dominated quasar 3C 275.1

We systematically analyze the 6-year {\it Fermi}/LAT data of the lobe-dominated quasars (LDQs) in the complete LDQ sample from 3CRR survey and report the discovery of high-energy $\gamma$-ray emission from 3C 275.1. The $\gamma$-ray emission of 3C 207 is confirmed and significant variability of the lightcurve is identified. We do not find statistically significant $\gamma$-ray emission from other LDQs. 3C 275.1 is the known $\gamma$-ray quasar with the lowest core dominance parameter (i.e., $R=0.11$). We also show that both the northern radio hotspot and parsec jet models can reasonably reproduce the $\gamma$-ray data. The parsec jet model, however, is favored by the potential $\gamma$-ray variability at the timescale of months. We suggest that some dimmer $\gamma$-ray LDQs will be detected in the future and LDQs could contribute non-negligibly to the extragalactic $\gamma$-ray background.Comment: 26 pages, 10 figures, 3 tables; ApJ in pres

Investigation on the Aggregation Behaviors and Filament Morphology of Tau Protein by a Simple 90° Angle Light-Scattering Assay

The in vitro aggregation of tau constructs was monitored by a simple 90° angle light-scattering (LS) approach which was conducted directly on fluorescence instrument. At the optimum incident wavelength (550 nm, unpolarized), the sensitivity of LS was high enough to detect tau aggregation at micromolar range. The nucleation and elongation, different events in the aggregation process of 4RMBD construct (corresponding with the four repeated units of tau Microtubule Binding Domain) could be observed by this approach, as compared with ThS fluorescence assay. The validity of this technique was demonstrated over a range of tau concentrations with different tau filaments. Linear regression of scattering light against concentration yielded the x-intercept, the critical concentrations of tau constructs. The critical concentrations of 4RMBD and its S305N mutant are 5.26 μM and 4.04 μM respectively, indicating point mutation S305N, which is associated with FTDP-17, appear to enhance the heparin-induced tau aggregation in vitro. Furthermore, the slopes of concentration dependence curves, as well as the angle dependence, were discussed based on the filaments morphology examined by electron microscopy and ultrasonication experiment