Expression of androgen and estrogen receptors in the testicular tissue of chickens, quails and chicken-quail hybrids

36 New Roman cocks, 30 Korean male quails and 30 chicken-quail hybrids of different day-age were selected and their body weight and testes weights were measured and as well, their testes were collected. Real-time polymerase chain reaction (RT-PCR) was performed to evaluate the messenger ribonucleic acid (mRNA) expression patterns of androgen receptors (AR) and estrogen receptors (ER) genes in testicular tissue of chickens, quails and chicken-quail hybrids at different growth stages. The results show that the testes of chickens and quails grew and developed normally with body weight gain, but the testes of chicken-quail hybrids had a slower growth rate and stunted growth. Real-time PCR showed AR and ER mRNA expression patterns in testes of chickens and quails at different growth stages were similar. AR mRNA expression in chickens and quails reached a significant peak at 80 and 30 days of age, respectively and their ER gene expression showed fluctuation slightly. The AR and ER expression of chicken-quail hybrids were different from the above expression patterns; the hybrids AR gene expression showed a gradual decline and ER gene expression gradually increased. The chickenquail hybrids AR and ER gene expression was abnormal and we speculate this is an important molecular factor for the testicular dysplasia of chicken-quail hybrids. Our results show that AR gene expression was upregulated by ER gene and we suggest that the synergetic effect of AR and ER gene regulated the normal testis growth and development of chicken and quail.Keywords: Chicken, quail, chicken-quail hybrid, testis, androgen receptors (AR), estrogen receptors (ER) expressionAfrican Journal of Biotechnology Vol. 11(29), pp. 7344-7353, 10 April, 201

Recurrent metastatic retroperitoneal dedifferentiated liposarcoma: a case report and literature review

Abstract Background Retroperitoneal liposarcoma (RPLPS), a rare tumor, is often treated using surgical procedures as the first choice for treatment. However, there is no consensus on the scope of surgical resection. In addition, the treatment outcomes of conventional radiotherapy and chemotherapy have not been satisfactory, particularly for specific types of LPS, such as dedifferentiated LPS. In this case study, we present a brief review of other cases of RPLPS, highlighting the selection of surgical scope for RPLPS and related adjuvant treatment for advanced RPLPS. Case presentation A case study is reported regarding an extremely rare recurrent and metastatic retroperitoneal dedifferentiated LPS. The primary RPLPS tumor, with a diameter of 20 cm and a weight of 2.5 kg, occupied the whole left abdomen and adhered to the left kidney. A surgical tumor resection combined with a left nephrectomy is performed. During the 6th -month postoperative follow-up examination, we observed the local recurrence of the tumor in the operation area, in addition to multiple metastatic tumors in both lungs. Further, the prescribed 3-month targeted treatment with anlotinib significantly reduced the size of the metastatic pulmonary tumors. However, the recurrent retroperitoneal tumors showed no significant change in size. Eventually, we observed no substantial evidence of tumor progression, with the patient’s condition under control. Conclusion The case demonstrated that the postoperative recurrence of widespread RPLPS required R0 resection to cure the disease, considering targeted therapy for advanced RPLPS control