The migration and the adhesion capacity of BMSCs.

<p>(A) The migration capacity was examined by the transwell migration assays. The BMSCs were attracted with or without SDF-1 on the inserts and were stained with crystal violet (upper). Quantitative data of the migratory BMSCs were counted in 5 high-power fields (lower). MAEC was the positive group. Values are means±SEM. *p<0.05 versus control group. Results are representative of three separate experiments. (B) The adhesion capacity was examined by TNF-α on fibronectin. The adhesion BMSCs on the fibronectin were counted in 8 different fields. MAEC was the positive group. Values are means±SEM. *p<0.05 versus control group. Results are representative of three separate experiments.</p

The mRNA expression levels of ICAM-1 and VCAM-1 in BMSCs.

<p>(A) BMSCs were treated with or without 10 ng/ml VEGF for 24 h. Relative ICAM-1 and VCAM-1 mRNA expression levels were determined by RT-PCR. Values of mRNA amounts were normalized to GAPDH expression and expressed relative to control (n=6). Error bars represent SEM (* P<0.05). (B) Relative ICAM-1 and VCAM-1 protein expression levels were determined by western blotting. Values of protein amounts were normalized to β-actin expression and expressed relative to control (n=3). Error bars represent SEM (* P<0.05). (C) The immunofluorescence analysis of PECAM-1, vWF and KDR expression in BMSCs. BMSCs were stained for KDR, vWF and PECAM-1 with Cy3 and were stained for nucleus with Honchest33258. Scale bars represent 100μm. (D) The FACS analysis of PECAM-1, vWF and KDR expression in BMSCs and the quantification were counted. Isotype control staining was shown in shade in representative histograms (n = 3).</p

Characteristics of BMSCs.

<p>(A) Representative images showed the morphology of BMSCs at passage 1, 3 and 8 under a microscope. Scale bars represent 100μm. (B) Representative images of FACS analysis showed that BMSCs expressed some maker for CD44, c-kit, CD105 and Sca-1. Isotype control staining was shown in shade in representative histograms (n = 3). </p