Additional file 1 of Response mechanism of carbon metabolism of Pinus massoniana to gradient high temperature and drought stress

Additional file 1

Plasmon Absorption of Au-in-CoAl₂O₄ Linear Nanopeapod Chains

We investigated the plasmon absorption of Au-in-CoAl₂O₄ linear nanopeapod chains experimentally and theoretically. The Au-in-CoAl₂O₄ nanopeapods with uniform Au nanospheres (25–40 nm in radius) were synthesized by pulsed electrodeposition, followed by heat treatment, and the plasmon absorption peaks of the Au nanospheres (AuNS) embedded in CoAl₂O₄ red-shifted due to the size effect of the AuNS and the effect of the cladding dielectric medium. As a result of localized surface plasmon resonance, the plasmon absorption of the peapods can be modulated by tuning the size of the AuNS in CoAl₂O₄, and the experimental data are in good agreement with the calculated results based on Mie theory. In addition, we carried out the first-principles calculation based on the density functional theory with the LDA+<i>U</i> scheme to study the electronic structure and dielectric properties of CoAl₂O₄, and the theoretical results are consistent with the experimental data, which proves that LDA+<i>U</i> is a good approximation for CoAl₂O₄ or other spinels containing transition elements

Gene Ontology classification.

<p>Genes were assigned to three categories: cellular components, molecular functions, and biological process.</p

GC content and average sequencing depth of the genome data used for assembly.

<p>The x-axis was GC content percent across every 10-kb non-overlapping sliding window.</p

Statistics of gene information.

<p>Statistics of gene information.</p

K-mer (k = 17) analysis for estimating the genome size of <i>R</i>. <i>roxburghii</i>.

<p>The x-axis is depth (X); the y-axis is the proportion that represents the frequency at that depth divided by the total frequency of all depths. The genome size was estimated by using the formula: Genome size = K-mer num/Peak depth, and the heterozygosis rate causes a sub-peak at a position half of that of the main peak, whereas a certain repeat rate can cause a similar peak at the position of multiple integers of the main peak.</p

Statistics of the assembled genome sequences.

<p>Statistics of the assembled genome sequences.</p

The proposed AsA synthetic and recycling pathways in higher plants.

<p>The four pathway included GalUA (D-galacturonic acid) pathway, Gal (L-galactose) pathway, Gulose(L-gulose) pathway and MI (Myo-inositol) pathway which catalyzed by GUR (D-galacturonate reductase), GME (GDP–D–Mannose-3,5-epimerase), GGP (GDP-L-galactose guanyltransferase), GPP (L-galactose-1-phosphate phosphatase), GDH (L-galactone dehydrogenase), GLDH (L-Galactono-lactone dehydrogenase) and MIOX(myo-inositol oxygenase). The recycling pathways were catalyzed by APX (ascorbate peroxidase), AAO (ascorbate oxidase), MDAR (mono-dehydroascorbate reductase) and DHAR (dehydroascorbate reductase).</p

Venn diagram showing the number of gene clusters in <i>R</i>. <i>roxburghii</i> and other close species, i.e., <i>M</i>.<i>×domestica</i>, <i>P</i>. <i>persica</i>, <i>P</i>. <i>bretschneideri</i>, and <i>P</i>. <i>mume</i>.

<p>The first number under the species name is the total number of putative genes subjected to clustering. The second number is the clustered family number. The overlapping areas represent sequences clustered with other species, and the number of non-overlapping areas represents specific genes.</p

Percentage of different motifs in trinucleotide repeats in <i>R</i>. <i>roxburghii</i>.

<p>Percentage of different motifs in trinucleotide repeats in <i>R</i>. <i>roxburghii</i>.</p