908 research outputs found
4-[3-(ChloroÂmethÂyl)-1,2,4-oxadiazol-5-yl]pyridine. Corrigendum
Corrigendum to Acta Cryst. (2007), E63, o4654
The role of cytochrome c oxidase subunit Va in non-small cell lung carcinoma cells: association with migration, invasion and prediction of distant metastasis
BACKGROUND: Lung cancer is one of the most lethal malignancies worldwide, but useful biomarkers of lung cancer are still insufficient. The aim of this study is to identify some membrane-bound protein(s) associated with migration and invasion in human non-small cell lung cancer (NSCLC) cells. METHODS: We classified four NSCLC cell lines into high and low migration/invasion groups by Transwell and Matrigel assays. Using two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), we identified 10 membrane-associated proteins being significantly overexpressed in the high migration/invasion group. The expression of the target protein in the four NSCLC cell lines was then confirmed by reverse transcription polymerase chain reaction (RT-PCR), western blot and immunostaining. RNA interference technique was applied to observe the influence of the target protein on migration and invasion. Gelatin zymography was also performed to evaluate the activities of matrix metalloproteinase (MMP)-2 and MMP-9. Expression condition of the target protein on surgical specimens was further examined by immunohistochemical staining and the clinicopathologic data were analyzed. RESULTS: We identified a mitochondria-bound protein cytochrome c oxidase subunit Va (COX Va) because of its abundant presence found exclusively in tumorous areas. We also demonstrated that migration and invasion of NSCLC cells decreased substantially after knocking down COX Va by siRNA. Meanwhile, we found a positive correlation between COX Va expression, Bcl-2 expression and activities of MMP-2 and MMP-9 in NSCLC cells. Immunohistochemical staining of surgically resected lung adenocarcinomas in 250 consecutive patients revealed that strong COX Va expression was found in 54.8% (137/250) of patients and correlated positively with the status of lymph node metastasis (P = 0.032). Furthermore, strong COX Va expression was associated with the presence of distant metastasis (P = 0.033). CONCLUSIONS: Our current study showed that COX Va may play a role in migration and invasion of NSCLC cells and can be used as a biomarker to predict aggressiveness of NSCLC
Fe-based metallic glasses and dyes in fenton-like processes: Understanding their intrinsic correlation
Fe-based metallic glasses have been demonstrated as effective heterogeneous catalysts in Fenton-like processes for dye degradation. Yet, currently corresponding studies have limitations due to the limited study object (dyes) and the correlation between metallic glasses and dye pollutants in Fenton-like processes is still not comprehensively studied. Accordingly, this work intensively investigated the thermal catalytic behavior correlations between two Fe-based metallic glasses (Fe78Si9B13 and Fe73.5Si13.5B9Cu1Nb3) and eight different dyes. Results indicated a lower activation energy in the more active metallic glass and a dependence of the activation energy of Fe-based metallic glasses in dye solutions. In addition, a high H2O2 concentration led to a declined catalytic efficiency but a photo-enhanced Fenton-like process overcame this limitation at high concentration of H2O2 due to the decrease of pH and enhancement of irradiation. Furthermore, the average mineralization rates of Fe78Si9B13 and Fe73.5Si13.5B9Cu1Nb3 have been measured to be 42.7% and 12.6%, respectively, and the correlation between decolorization and mineralization revealed that a faster decolorization in a Fenton-like process contributed to a higher mineralization rate. This work provides an intrinsic viewpoint of the correlation between Fe-based metallic glasses and dyes in Fenton-like processes and holds the promise to further promote the industrial value of metallic glasses
Human Comprehensible Active Learning of Genome-Scale Metabolic Networks
An important application of Synthetic Biology is the engineering of the host
cell system to yield useful products. However, an increase in the scale of the
host system leads to huge design space and requires a large number of
validation trials with high experimental costs. A comprehensible machine
learning approach that efficiently explores the hypothesis space and guides
experimental design is urgently needed for the Design-Build-Test-Learn (DBTL)
cycle of the host cell system. We introduce a novel machine learning framework
ILP-iML1515 based on Inductive Logic Programming (ILP) that performs abductive
logical reasoning and actively learns from training examples. In contrast to
numerical models, ILP-iML1515 is built on comprehensible logical
representations of a genome-scale metabolic model and can update the model by
learning new logical structures from auxotrophic mutant trials. The ILP-iML1515
framework 1) allows high-throughput simulations and 2) actively selects
experiments that reduce the experimental cost of learning gene functions in
comparison to randomly selected experiments.Comment: Invited presentation for AAAI Spring Symposium Series 2023 on
Computational Scientific Discover
Are Proselfs More Deceptive and Hypocritical? Social Image Concerns in Appearing Fair
Deception varies across individuals and social contexts. The present research explored how individual difference measured by social value orientations, and situations, affect deception in moral hypocrisy. In two experiments, participants made allocations between themselves and recipients with an opportunity to deceive recipients where recipients cannot reject their allocations. Experiment 1 demonstrated that proselfs were more deceptive and hypocritical than prosocials by lying to be apparently fair, especially when deception was unrevealed. Experiment 2 showed that proselfs were more concerned about social image in deception in moral hypocrisy than prosocials were. They decreased apparent fairness when deception was revealed and evaluated by a third-party reviewer and increased it when deception was evaluated but unrevealed. These results show that prosocials and proselfs differed in pursuing deception and moral hypocrisy social goals and provide implications for decreasing deception and moral hypocrisy
Comprehensive study of the blazars from Fermi-LAT LCR: The log-normal flux distribution and linear RMS-Flux relation
Fermi-LAT LCR provide continuous and regularly-sampled gamma-ray light
curves, spanning about 14 years, for a large sample of blazars. The log-normal
flux distribution and linear RMS-Flux relation of the light curves for a few of
Fermi blazar have been examined in previous studies. However, the probability
that blazars exhibit log-normal flux distribution and linear RMS-Flux relation
in their gamma-ray light curves has not been systematically explored. In this
study, we comprehensively research on the distribution of gamma-ray flux and
the statistical characteristics on a large sample of 1414 variable blazars from
the Fermi-LAT LCR catalog, including 572 FSRQs, 477 BL Lacs, and 365 BCUs, and
statistically compare their flux distributions with normal and log-normal
distributions. The results indicate that the probability of not reject
log-normal is 42.05% for the large sample, and there is still 2.05% probability
of not reject normality, based on the joint of Kolmogorov-Smirnov, Shapiro-Wilk
and Normality tests. We further find that the probability that BL Lacs conforms
to the log-normal distribution is higher than that of FSRQs. Besides, after
removing sources with less than 200 data points from this large sample, a
sample of 549 blazars, which is still a large sample comparing to the previous
studies, was obtained. Basing on dividing the light curves into segments every
20 points (or 40 points, or one year), we fitted the linear RMS-Flux relation
of this three different sets, and found that the Pearson correlation
coefficients are all close to 1 of the most blazars. This result indicates a
strong linear correlation between the RMS and the flux of this 549 blazars. The
log-normal distribution and linear RMS-Flux relation indicate that the
variability of gamma-ray flux for most blazars is non-linear and multiplicative
process.Comment: 13pages, 5figures, Accepted for publication in RA
Optimum Structure Adjustment for Flexible Fluorescent and Phosphorescent Organic Light Emitting Diodes
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