Mucosal NF-κB expression in each group at 4 and 7 days after MSCs administered.

<p>(<b>A</b>) Semiquantitative assessment of bands for NF-κB and its internal control protein. (<b>B</b>) Values of the NF-κB band performed densitometrically using the software Quantity One. Each bar represents the mean ± SD of the different groups; values were compared by one-way ANOVA test, <i>**: P<0.01</i>, <i>*: P<0.05</i>. S: sham group; C: control (saline) group; M: MSCs group. 4d and 7d represent 4 days and 7 days postoperatively.</p

Expression of pERK1/2 in intestinal mucosa at 4 and 7 days after MSCs transplantation.

<p>(<b>A</b>) Bands of the expression of pERK1/2 and its own tERk1/2 protein were detected by western blot. (<b>B</b>) The ratio value of pERK1/2 to its own tERK1/2 of the bands which was evaluated densitometrically using the software Quantity One for the three groups at different time points. Each bar represents mean ± SD of the ratio value in every group. S: sham group; C: control (saline) group; M: MSCs group. 4d and 7d represent 4 days and 7 days postoperatively. *<i>: P<0.05</i>, compared with control (saline) group, by one-way ANOVA test.</p

MSCs homing photograph (40×).

<p>After cell transplantation, engrafted cells were detected by Y chromosome <i>in </i><i>situ</i> hybridization at 1, 4, 7, and 10 days (the green fluorenscent point represents positive donor derived cells), which corresponded to graphs A, B, C, and D respectively. Most of the homed cells were located at mucosal lamina propria, and the amounts of engrafted cells were more at 4 and 7days than 1 and 10 days postoperatively.</p

Real-Time PCR analysis of PCNA expression in the intestinal mucosa.

<p>Data are means ± SD of each group at different time point and expressed as the ratio of original value mRNA of PCNA to its internal control mRNA of GAPDH. S: sham group; C: control (saline) group; M: MSCs group. 4d and 7d represent 4 days and 7 days postoperatively. ** <i>P<0.01</i>, <i>* P<0.05</i>, compared with control (saline) group, by one-way ANOVA test.</p

ELISA analysis of intestinal mucosal TNF-α content among the three groups.

<p>Data are shown as means ± SD of the three groups, <i>*P < 0.01</i> vs. MSC group; # <i>P <0.05</i> vs. Sham group, values are evaluated by one-way ANOVA test.</p