1 research outputs found
(3-Aminopropyl)-4-methylpiperazine End-capped Poly(1,4-butanediol diacrylate-co-4-amino-1-butanol)-based Multilayer Films for Gene Delivery
Biodegradable
polyelectrolyte surfaces for gene delivery were created through electrospinning
of biodegradable polycations combined with iterative solution-based
multilayer coating. Poly(β-amino ester) (PBAE) poly(1,4-butanediol
diacrylate-co-4-amino-1-butanol) end-capped with 1-(3-aminopropyl)-4-methylpiperazine
was utilized because of its ability to electrostatically interact
with anionic molecules like DNA, its biodegradability, and its low
cytotoxicity. A new DNA release system was developed for sustained
release of DNA over 24 h, accompanied by high exogenous gene expression
in primary human glioblastoma (GB) cells. Electrospinning a different
PBAE, poly(1,4-butanediol diacrylate-co-4,4′-trimethylenedipiperidine),
and its combination with polyelectrolyte 1-(3-aminopropyl)-4-methylpiperazine
end-capped poly(1,4-butanediol diacrylate-co-4-amino-1-butanol)-based
multilayers are promising for DNA release and intracellular delivery
from a surface