Fibroblast Cell Viability Effectiveness between the Highlands and Lowlands Coconut Water (Cocos Nucifera L.)

Tooth avulsion is a tooth that completely detached from its socket. The purpose of this study is to determine the differences of the effectiveness of coconut water in the dwarf variety from highlands and lowlands on fibroblast cell viability. Coconut water from each habitat was picked randomly. Samples were divided into five groups. Group 1 was the media control, group 2 was the cell control, group 3 was HBSS, group 4 was the coconut water from highlands, and group 5 was the coconut water from lowlands. To determine the BHK-21 fibroblast cell viability after 4 hours of submerging, MTT assay was used. The absorbance was read by ELISA reader with wavelength 620nm. The results were analyzed using One-way ANOVA with α=0.05. There are significant differences in each group. Coconut water from highlands has cell viability percentage of 8%, while the coconut water from lowlands has 54%. Based on the CD50% parameter, coconut water from the lowlands is more effective than coconut water from the highlands. The coconut water in the dwarf variety from the lowlands is more effective than the coconut water from the highlands towards BHK-21 fibroblast cell viability for 4 hours