Patient PBMC samples involved in proliferation assay.

*<p>HT = Hashimoto's thyroiditis. AR = allergic rhinitis.</p

Forest plot showing the association between <i>p16</i> methylation and overall survival of NSCLC.

<p>The summary HR and 95% CIs were shown (random-effects model analysis).</p

Circulating CD8⁺ CTLs are increased significantly in GV patients.

<p>(A) IFN-γ, GrB and Perforin–expressing cells were detected by intracellular staining. Values are the percentage of IFN-γ⁺ CD8⁺, GrB⁺ CD8⁺ or Perforin⁺ CD8⁺ cells among CD3⁺ T cells (Representative cases). (B) Flow cytometric analysis of IFN-γ⁺ CD8⁺, GrB⁺ CD8⁺ and Perforin⁺ CD8⁺ cells within the CD3⁺ T cell populations from patients with progressive GV (n = 38), stable GV (n = 12) and control subjects (n = 20). * = P<0.05, ** = P<0.01, *** = P<0.001. (C) The CD8⁺ CTL population of 6 patients was monitored longitudinally: The percentage of IFN-γ⁺ CD8⁺ T cells, GrB⁺ CD8⁺ T cells and Perforin⁺ CD8⁺ T cells was measured initially during GV progression and again following stabilization after treatment. * = P<0.01, ** = P<0.001.</p

Tregs suppress proliferation and cytokine production of autologous CD8⁺ T cells.

<p>(A) Detection of Treg suppression for CD8⁺ T cells. CFSE-labeled CD8⁺ T cells were stimulated in the presence of CD4⁺ CD25⁺ Treg cells at the indicated ratios for 5 days. Cellular division was measured by flow cytometric analysis based on the intensity of CFSE signal. Representative CFSE profiles from a GV patient and a normal control are shown. Values given represent the percentage of CFSE^low cells among CFSE⁺ T cells. (B) Treg cells are largely unresponsive to TCR stimulation. Unlabeled and CFSE labeled CD4⁺ CD25⁺ T cells were cultured with anti-CD3/CD28 Abs, under the same stimulatory conditions as above, but without autologous CD8⁺ T cells present. Values given are the percentage of CFSE^low cells among CFSE⁺ Treg cells (Representative cases). (C) Dose-dependent suppression of CD8⁺ T cell proliferation by CD4⁺ CD25⁺ T cells. The suppressive capacity of CD4⁺ CD25⁺ Tregs was compared between GV patients (n = 6) and normal control subjects (n = 4). * = P<0.05; ** = P<0.01. The production of IFN-γ (D) and TNF-α (E) was determined. CD8⁺ T cells were cultured with Tregs at various ratios in the presence of anti-CD3/CD28 Ab stimulation for 48 hours. IFN-γ and TNF-α release into the supernatant was determined by an enzyme-linked immunosorbent assay. The data were from 6 progressive GV individuals and 4 healthy controls. * = P<0.05; ** = P<0.01.</p

The main characteristics and results of eligible studies evaluating p16 hypermethylation and NSCLC patients’ survival.

<p>MSP, Methylation-specific PCR; Q-MSP, quantitative methylation-specific PCR; FFPE, formalin-fixed paraffin-embedded; ADC, adenocarcinoma; S, significant; NS,not significant; NA, not available.</p

Forest plot showing the association between <i>p16</i> methylation and disease-free survival of NSCLC.

<p>The summary HR and 95% CIs were shown (random-effects model analysis).</p

Patient skin biopsy samples for CD8⁺ cells and Foxp3⁺ cells quantification by immunohistochemistry.

*<p>SLE = systemic lupus erythematosus; AA = alopecia areata; AR = allergic rhinitis; HT = Hashimoto's thyroiditis.</p

Skin-infiltrating CD8⁺ cells and Foxp3⁺ cells in GV patients.

<p>(A) Consecutive sections were used for immunohistochemical detection of T cells expressing CD8 or Foxp3⁺ in skin samples from healthy controls or GV patients (representative fields, 200×). Positive cells appear brown. Both CD8⁺ (B) and Foxp3⁺ (C) T cell numbers were increased significantly in the perilesional (PL) tissues of progressive GV patients (n = 16). In contrast, no significant differences were observed between lesional (L) (n = 2) and non-lesional GV skin (NL) (n = 8), as compared to normal skin from unaffected adults (Cont) (n = 6). * = P<0.05, ** = P<0.01. (D) The number of CD8⁺ and Foxp3⁺ T cells in perilesional GV skin samples exhibited a positive correlation (<i>r</i> = 0.706, P<0.001; n = 16). Values in B–C are the mean and SD.</p

The results of meta-analysis on NSCLC overall survival and p16 methylation.

<p>MSP, Methylation-specific PCR; Q-MSP, quantitative methylation-specific PCR; FFPE, formalin-fixed paraffin-embedded; ADC, adenocarcinoma; Pts, patients.</p

Supplementary document for Visible and NIR microscopic hyperspectrum reconstruction from RGB images with deep convolutional neural networks - 6802670.pdf

Pictures of samples used in the experimen