Latent profile and network analysis of risk perception among a sample of Chinese university students during the COVID-19 pandemic: a cross-sectional and longitudinal study

BackgroundThe risk perception of contracting COVID-19 is an important topic for assessing and predicting COVID-19 infection and health education during the pandemic. However, studies that use latent profiles and network analysis together to measure the risk perception of COVID-19 are rare. Therefore, this study combined latent profile analysis and network analysis to measure risk perception toward COVID-19 among Chinese university students through a cross-sectional and longitudinal study.MethodsA sample of 1,837 Chinese university students (735 males, 40%) completed the cross-sectional study with an eight-item risk perception questionnaire in January 2020, while 334 Chinese university students (111 males, 33.2%) completed the longitudinal study at three time points.ResultsA two-class model including a low risk perception class (n = 1,005, 54.7%) and a high risk perception class (n = 832, 45.3%) was selected for the cross-sectional study. Nodes rp6 (“Average people have chances of contracting COVID-19'') and rp7 (“Average people worry about catching COVID-19”) had the strongest edge intensity (r = 0.491), while node rp5 (“The COVID-19 outbreak affects the whole country”) had the highest strength centrality in the cross-sectional study. The risk perception of contracting COVID-19 decreased continuously at the three time points. Moreover, the network structures and global strengths had no significant differences in the longitudinal study.ConclusionsThe risk perception of contracting COVID-19 decreased continually during the COVID-19 pandemic, which indicated the importance of cultural influence and effective government management in China. In addition, university students displayed strong trust and confidence in the government's ability to fight COVID-19. The results indicate that the government should take strong measures to prevent and intervene in various risks and reinforce the public's trust through positive media communications

Application of meso-2,3-Dimercaptosuccinic Acid Self-assembled Gold Electrode for Voltammetric Determination of Copper

Fabrication and electrochemical characteristics of the meso-2,3-dimercaptosuccinic acid (DMSA) self-assembled monolayer modified gold electrode were described. The modified electrode exhibited increased sensitivity and selectivity for CuII compared to the bare gold electrode by stripping voltammetry and the peak current was proportional to the concentration of CuII in the range of 8.0 10–7 1.2 10–4 mol/L with the detection limit of 1.1 10–7 mol/L. The influence of coexistent substances was investigated and the modified electrode showed good selectivity for copper determination. The DMSA/Au electrode was applied for CuII determination in a tap water sample with satisfactory results, with the recovery in the range from 99.7 to 101.1 %

Characterization of a sensitive biosensor based on an unmodified DNA and gold nanoparticle composite and its application in diquat determination

AbstractDNA usually adsorbs gold nanoparticles by virtue of mercapto or amino groups at one end of a DNA molecule. However, in this paper, we report a sensitive biosensor constructed using unmodified DNA molecules with consecutive adenines (CA DNA) and gold nanoparticles (GNPs). The CA DNA–GNP composite was fabricated on gold electrodes and characterized by using of scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and the electrochemical method. Using an electrochemical quartz crystal microbalance (EQCM), the mechanism by which the CA DNA and GNPs combined was also studied. The modified electrode exhibited an ultrasensitive response to diquat. Differential pulse voltammetry (DPV) was used to study the linear relationships between concentrations and reduction peak currents, ranging from 1.0×10−9M to 1.2×10−6M. The detection limit of it is 2.0×10−10M. The feasibility of the proposed assay for use in human urine and grain was investigated, and the satisfactory results were obtained

The Trait-State Fear of Missing Out Scale: validity, reliability, and measurement invariance in a Chinese sample of university students

Background: Research into the 'fear of missing out' (FoMO) has greatly increased in recent years. In Asia, many university students frequently use social networking sites (SNSs) via their smartphone. There has also been some studies examining problematic social media use, but there are few Chinese studies concerning FoMO. This may be partly due to the lack of standardized measurement tools for assessing FoMO. Therefore, the present study psychometrically validated the Chinese version of the Trait-State Fear of Missing Out Scale (T-SFoMOS-C) and tested its reliability, validity and measurement invariance among Chinese university students. Methods: A total of 2,017 university students (aged 17 to 25 years) completed an online survey including the Chinese Trait-State Fear of missing Out Scale (T-SFoMOS-C), the Social Network Site Intensity Scale (SNSIS), and the International Positive and Negative Affect Scale short-form (I-PANAS-SF). Results: Item analysis and exploratory factor analysis was carried out on the T-SFoMOS-C. Confirmatory factor analysis (χ2 = 177.49, df = 50, p < .01; TLI = .959; CFI = .960; SRMR = .038; RMSEA = .050) and measurement invariance showed that the T-SFoMOS-C for university students had good construct validity among different groups. The internal consistency of the T-SFoMOS-C (.81), the test-retest reliability (.81), and the composite reliability of state-FoMO and trait-FoMO (.76 and .80) were also good. The T-SFoMOS-C was significantly correlated with the SNSIS (.40) and the Negative Affect (NA) (.26), respectively. Conclusions: The T-SFoMOS-C is relatively reliable and valid among different groups, supporting its utility among Chinese university students

Relationship between gaming disorder, self-compensation motivation, game flow, time spent gaming, and fear of missing out among a sample of Chinese university students: a network analysis

Background and Aims: In previous correlational research, the relationship between gaming disorder (GD), compensation motivation, game flow, time spent gaming, and fear of missing out (FoMO) has been examined. However, network analysis has rarely been applied to explore the relationship between GD, self-compensation motivation, game flow, time spent gaming, and FoMO. Therefore, the present study used network analysis to examine the relationship between the aforementioned variables among a sample of gamers. Methods: The present study comprised gamers (N = 1,635) recruited from three Chinese universities, who completed an online survey including the Gaming Disorder Test, Self-Compensation Motivation Questionnaire, Game Flow Questionnaire, and Trait-State Fear of Missing Out Scale, as well as four items related to time spent gaming. Results: Self-compensation motivation, game flow, time spent gaming, and FoMO were all significantly and positively associated with GD. In the domain-level and facet-level networks, weekday gaming hours and weekend gaming hours had the strongest edge intensity. The domain-level, facet-level, and item-level networks analysis also showed that GD was connected with self-compensation motivation, game flow, time spent gaming, and FoMO. The network structure demonstrated a significant difference between males and females (2.33 vs. 2.81, p = 0.001) using the domain-level network comparison test (NCT). Conclusions: The results suggest that GD is closely associated with self-compensation motivation, game flow, time spent gaming, and FoMO. FoMO and gaming motivation (i.e., self-compensation and game flow) may increase time spent gaming and facilitate GD. Therefore, interventions that decrease game immersion and time spent gaming are likely to decrease GD

Influence of voluntary action and outcome valence on the sense of agency

Recent studies have revealed that people might experience a lessened sense of agency for negative consequences by claiming that they were obeying orders. However, little is known about the cognitive neural mechanism behind the reduced sense of agency when individuals are forced to inflict physical harm on others. This study adopted temporal estimation tasks to investigate the internal mechanism of voluntary action on the sense of agency and the moderating effect of outcome valence as measured by event-related potentials (ERPs). In the temporal estimation task, participants were asked to make trade-offs of monetary gains for themselves against painful electric stimuli experienced by strangers, subjectively estimated the perceptual temporal interval between keypress actions (i.e., free or coercive actions) and consequent outcomes (i.e., positive or negative tones) and rated the feeling of control. The results showed that perceived temporal interval was shorter for positive tones compared with negative tones in the coercive condition, and induced more negative N1 and N300 amplitudes, which indicated that the implicit sense of agency was higher. However, the explicit sense of agency was stronger in the free condition than in the coercive condition, which was not influenced by outcome valence. We discuss the implications of utilizing positive feedback and free choice as significant strategies for those experiencing the abnormal sense of agency

Effect of all-trans retinoic acid on the proliferation and differentiation of brain tumor stem cells

<p>Abstract</p> <p>Objective</p> <p>To investigate the effect of all-trans retinoic acid(ATRA) on the proliferation and differentiation of brain tumor stem cells(BTSCs) <it>in vitro</it>.</p> <p>Methods</p> <p>Limiting dilution and clonogenic assay were used to isolate and screen BTSCs from the fresh specimen of human brain glioblastoma. The obtained BTSCs, which were cultured in serum-free medium, were classified into four groups in accordance with the composition of the different treatments. The proliferation of the BTSCs was evaluated by MTT assay. The BTSCs were induced to differentiate in serum-containing medium, and classified into the ATRA group and control group. On the 10^thday of induction, the expressions of CD133 and glial fibrillary acidic protein (GFAP) in the differentiated BTSCs were detected by immunofluorescence. The differentiated BTSCs were cultured in serum-free medium, the percentage and the time required for formation of brain tumor spheres (BTS) were observed.</p> <p>Results</p> <p><b>BTSCs </b>obtained by limiting dilution were all identified as CD133-positive by immunofluorescence. In serum-free medium, the proliferation of BTSCs in the ATRA group was observed significantly faster than that in the control group, but slower than that in the growth factor group and ATRA/growth factor group, and the size of the BTS in the ATRA group was smaller than that in the latter two groups(<it>P </it>< 0.01). In serum-containing medium, the expression percentages of CD133 and GFAP in the differentiated BTSCs were (2.29% ± 0.27%) and (75.60% ± 4.03%) respectively in the ATRA group, and (7.05% ± 0.49%) and (12.51% ± 0.77%) respectively in the control group. The differentiation rate of BTSCs in the ATRA group was significantly higher than that in the control group (<it>P </it>< 0.05), but there was still CD133 expressed in the ATRA group. The differentiated BTSCs could re-form BTSs in serum-free medium. The percentage of BTS formation in the ATRA group was(4.84% ± 0.32%), significantly lower than that in the control group (17.71% ± 0.78%) (<it>P </it>< 0.05), and the time required for BTS formation in the ATRA group was (10.07 ± 1.03)d, significantly longer than that in the control group (4.08 ± 0.35)d (<it>P </it>< 0.05).</p> <p>Conclusion</p> <p><b>ATRA </b>can promote the proliferation and induce the differentiation of BTSCs, but the differentiation is incomplete, terminal differentiation cannot be achieved and BTSs can be formed again.</p

Identification of subtype-specific metastasis-related genetic signatures in sarcoma

Background: Sarcomas are heterogeneous rare malignancies constituting approximately 1% of all solid cancers in adults and including more than 70 histological and molecular subtypes with different pathological and clinical development characteristics. Method: We identified prognostic biomarkers of sarcomas by integrating clinical information and RNA-seq data from TCGA and GEO databases. In addition, results obtained from cell cycle, cell migration, and invasion assays were used to assess the capacity for Tanespimycin to inhibit the proliferation and metastasis of sarcoma. Results: Sarcoma samples (N = 536) were divided into four pathological subtypes including DL (dedifferentiated liposarcoma), LMS (leiomyosarcoma), UPS (undifferentiated pleomorphic sarcomas), and MFS (myxofibrosarcoma). RNA-seq expression profile data from the TCGA dataset were used to analyze differentially expressed genes (DEGs) within metastatic and non-metastatic samples of these four sarcoma pathological subtypes with DEGs defined as metastatic-related signatures (MRS). Prognostic analysis of MRS identified a group of genes significantly associated with prognosis in three pathological subtypes: DL, LMS, and UPS. ISG15, NUP50, PTTG1, SERPINE1, and TSR1 were found to be more likely associated with adverse prognosis. We also identified Tanespimycin as a drug exerting inhibitory effects on metastatic LMS subtype and therefore can serve a potential treatment for this type of sarcoma. Conclusions: These results provide new insights into the pathogenesis, diagnosis, treatment, and prognosis of sarcomas and provide new directions for further study of sarcoma

Circular Polarization in two Active Repeating Fast Radio Bursts

Fast radio bursts (FRBs) are bright millisecond radio bursts at cosmological distances. Only a small fraction of FRBs apparently repeat. Polarization, a fundamental property of electromagnetic signals, often carries critical information about the radiation processes, the environment, and the intervening medium of FRBs. Here we report circular polarization detections of two active repeating FRBs, namely FRBs 20121102A and 20190520B, with the Five-hundred-meter Aperture Spherical radio Telescope. We detect circular polarization in both active repeating FRBs, which increases the number of repeating FRBs with circular polarization to three. In one of the bursts of FRB 20121102A, we detect 64% degree of circular polarization. The observed circular polarization is unlikely induced by multipath propagation. Our observations favor circular polarization induced by Faraday conversion or radiation mechanism intrinsic to the FRB source. The conditions to generate circular polarization have to be rare in either case.Comment: 9pages, 3 figures, and 1 table, published in Science Bulleti

FRBs' Brownian Motion on Time-Energy Bivariate Space

The origin of fast radio bursts (FRBs), the brightest cosmic explosion in radio bands, remains unknown. Magnetar-related mechanisms are currently favored. The searches for short-term periodicity that is naturally expected for such fast-spinning compact objects, however, have failed. We introduce here a novel method for a comprehensive analysis of active FRBs' behaviors in the time-energy domain. Using ``Pincus Index'' and ``Maximum Lyapunov Exponent'', we were able to quantify the stochasticity and chaos, respectively, of the bursting events and put FRBs in the context of common transient physical phenomena, such as pulsars, earthquakes, and solar flares. In the bivariate time-energy domain, repeated FRB bursts' behaviors deviate significantly (more random, less chaotic) from pulsars, earthquakes, and solar flares. FRB bursts wander in time-energy space stochastically, akin to Brownian motions. The high degree of stochasticity suggests complex and even multi-origins for FRBs.Comment: 6 pages, 4 figures, submitte