Postoperative influences of the torsional phacoemulsification on foveal thickness and corneal edema

AIM: To report the influences onfoveal thickness and corneal edema after torsional phacoemulsification.<p>METHODS: Totally 52 patients(52 eyes)with age-related cataract were randomly assigned to phacoemulsification using torsional mode(26 eyes)or conventional ultrasound mode(26 eyes). The foveal thickness examined by optical coherence tomography(OCT)after surgery at 1, 4 and 12wk and corneal edema was examined by slit lamp after surgery at 1d.<p>RESULTS: The postoperative averagefoveal thickness datas of the two groups, comparing with corresponding preoperative datas, were significantly augmented at 1, 4 and 12wk(<i>P</i><0.05); the difference of foveal thickness was obviously augmented at 1wk postoperatively(<i>P</i><0.05), was no obvious differences at 4 and 12wk(<i>P</i>>0.05). The effects of corneal edema in torsional group were slighter(<i>P</i><0.05). <p>CONCLUSION: The postoperative influences onfoveal thickness and corneal edema with torsional mode are slighter than that with ultrasound mode, and the postoperative reactions with torsional mode are efficiently reduced

Adversarial Noise Layer: Regularize Neural Network By Adding Noise

In this paper, we introduce a novel regularization method called Adversarial Noise Layer (ANL) and its efficient version called Class Adversarial Noise Layer (CANL), which are able to significantly improve CNN's generalization ability by adding carefully crafted noise into the intermediate layer activations. ANL and CANL can be easily implemented and integrated with most of the mainstream CNN-based models. We compared the effects of the different types of noise and visually demonstrate that our proposed adversarial noise instruct CNN models to learn to extract cleaner feature maps, which further reduce the risk of over-fitting. We also conclude that models trained with ANL or CANL are more robust to the adversarial examples generated by FGSM than the traditional adversarial training approaches

Synergistic Effect of Trehalose and Saccharose Pretreatment on Maintenance of Lyophilized Human Red Blood Cell Quality

Purpose: To investigate the synergistic effect of trehalose and saccharose pretreatment on maintenance of lyophilized human red blood cell (RBC) quality.Methods: RBCs were pre-treated with trehalose and saccharose, and then lyophilized and re-hydrated. Prior to lyophilization and after re hydration, RBC parameters, RBC counts, total hemoglobin concentration, mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), comprehensive deformation index, hemolysis ratio and phosphatidylserine (PS) expression, were determined using a hematology analyzer, an RBC deformation instrument, a spectrophotometer and a flow cytometer, respectively. Superoxide dismutase (SOD), glucose-6-phosphate dehydrogenase (G-6-PD), and adenosine triphosphatase (ATPase) activities were determined using kits for SOD, ATPase, and G-6- PD assay, respectively.Results: After lyophilization-rehydration, RBC counts and total hemoglobin recovery rates, deformability, and RBC SOD, ATPase, and G-6-PD activities were significantly decreased by 47.24 – 74.65 % (p &lt; 0.01), compared with the normal group. RBC osmotic fragility and PS expression on the outer surface of the RBC membrane were significantly increased by 168.53 and 629.30 % (p &lt; 0.01), respectively, compared with the normal group. RBC MCH and MCV values were not significantly affected by lyophilization rehydration (p &gt; 0.05). Trehalose and saccharose pretreatment significantly reversed the effects of lyophilization-rehydration on these RBC parameters by approximately 13.16 – 211.11 % (p &lt; 0.01), compared with the control group. The combined effects were synergistic.Conclusion: Trehalose and saccharose pretreatment synergistically enhances maintenance of lyophilized RBC quality.Keywords: Trehalose, Saccharose, Lyophilization, Red blood cell, Hematological parameter

Protocol for profiling in vitro intratumor heterogeneity using spatially annotated single-cell sequencing

Here, we present a protocol for spatially annotated single-cell sequencing, a technique for spatially profiling intratumor heterogeneity with deep single-cell RNA sequencing and single-cell resolution. By combining live-cell imaging and photopatterned illumination, we describe steps to identify regions of interest in an in vitro tumor model, label the selected cells with photoactivatable dyes, and isolate and subject them to scRNAseq. This protocol can be applied to a range of cell lines and could be expanded to tissue sections. For complete details on the use and execution of this protocol, please refer to Smit et al. (2022).1</p

Design and clinical application of colonoscopy convenient pant

结肠镜检查是诊治肠道疾病的首选方法。随着肠道疾病发病率的增长，越来越多的人群需要接受结肠镜检查。为了方便检查、保护病人隐私及防止受凉等，笔者设计制作了结肠镜检查方便裤，并已经很好地将其应用于临床。Colonoscopy is the first choice for diagnosis and treatment of intestinal diseases. With the growth of the incidence of intestinal disease, more and more people need colonoscopy. In order to inspecting conveniently, protecting patients’ privacy and preventing them from catching a cold, we designed the colonoscopy convenient pant and have applied it into clinic well