Association of methylenetetrahytrofolate reductase (MTHFR) C677T and A1298C polymorphisms with the susceptibility of childhood acute lymphoblastic leukaemia (ALL) in Chinese population

BACKGROUND: The aim of this study was to investigate the relationship between the polymorphisms of the methylenetetrahytrofolate reductase (MTHFR) gene and susceptibility to childhood acute lymphoblastic leukemia (ALL). METHODS: A case–control study was conducted among 98 children with ALL and 93 age- and sex- matched non-ALL controls. Genotyping of MTHFR C677T and A1298C polymorphisms was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The odds ratios (ORs) of MTHFR genotypes were used to assess the associations of these polymorphisms with childhood ALL susceptibility. RESULTS: No significant differences were observed for frequencies of the 677CC, 677CT and 677TT genotypes between patients and controls. Frequencies of the 1298AA, 1298 AC and 1298CC genotypes between the two groups were significantly different. The risk of ALL with the 1298C allele carriers (AC + CC) was elevated by 1.1 times compared with the AA genotype [OR = 2.100; 95% CI (1.149; 3.837); P = 0.015]. CONCLUSIONS: The MTHFR A1298C polymorphism is associated with susceptibility to childhood ALL in the Chinese population

Status quo and development trend of selenium-rich foxtail millet industry in China

To analyze the status of selenium-enriched areas, main producing area of foxtail millet and selenium-rich foxtail millet products in China, statistical data, literature, network resources, expert interview, field investigation, etc. are used to acquire data. The data is cleared up and analyzed, and selenium-rich foxtail millets suitable for different regions to plant are screened out, then the development direction is pointed out, which provides a reference for improving the industrialization level of selenium-rich foxtail millet in China

Application of Biogas in North China

Energy issues are an important part of the agricultural system, and they are closely related to rural production and peasant life. The state of rural energy development plays an important role in the development of rural ecology, economy and society in China, and is also an important indicator of the sustainable development capability of China’s agriculture. This article analyzes the principle of the rural energy-ecology model in the north and its role in the sustainable development of agriculture, and puts forward some views on its existing problems

Analysis of the Influencing Factors of Changes in Foxtail Millet Planting Area in Hebei Province

Since reform and open to the outside world, foxtail millet planting area in Hebei Province has constantly reduced, and its planting region has changed from the whole province into some regions, with obvious changes. In this paper, by using qualitative and quantitative approaches like chart analysis, summarizing, Delphi method and osculating value model, the influencing factors of changes in millet growing region in Hebei Province were studied. The results showed that the changes of millet planting area were closely related to many factors, such as little investment in scientific research, weak policy support, a single consumer product demand, the rapid development of other staple crops, labor migration, relatively low comparative effectiveness and physiological characteristics of the millet itself. Then some suggestions were put forward, such as increasing research support, strengthening scientific research strength, researching and developing deeply processed products, expanding consumer groups, improving millet policy support, providing subsidies for planting the improved varieties, researching and developing various types of machinery, and increasing millet production technology training

Analysis of Nutritional Ingredients and Planting Techniques of Quinoa

Quinoa (Chenopodium quinoa Willd.) is native to South America, and its grains are mainly used. In recent years, using quinoa seedlings as a vegetable have become more and more popular. In this paper, the biological characteristics, nutritional ingredients and health functions of quinoa were introduced in detail, and on the basis of experiments and production, a set of scientific cultivation techniques was summarized to promote the large-scale cultivation of this characteristic vegetable

A Simple and High Quality Method for Isolation and Extraction of Total RNA of Pholiota adipose

Analyzing functional values of RNA using RNA-seq technology is a hot spot of researches. In order to study the medicinal value of Pholiota adipose from the transcriptome level, it is necessary to extract and isolate RNA samples of high purity and high quality P. adipose. In this study, liquid nitrogen grinding Trizol one-step method was used to extract the total RNA of P. adipose. Quality test and statistical comparative analysis were carried out for RNA extract of liquid nitrogen grinding treated and untreated P. adipose. The results showed that the concentration of RNA in the samples treated with liquid nitrogen was much higher than that of the samples without grinding treatment. The OD260/280 of both was about 2, indicating that the purity of RNA was very high. Besides, the ratio of fluorescence intensity of 25S and 18S subunit strips of three replicate samples was 1.8, 1.9, and 1.9, close to 2, indicating RNA integrity is good. RIN test results of Agilent 2100 were 9.1, 8.7, and 9.3, higher than the standard value 6.8, further proving the integrity. In sum, liquid nitrogen grinding Trizol one-step method is a very simple and efficient method for extracting high quality total RNA of P. adipose

Characteristics of Cheuopodium quinoa and Development Recommendations

Cheuopodium quinoa is annual Chenopodiaceae Cheuopodium herbaceous dicotyledon. It originated in the Andes in South America and has a planting history of more than 5000 years. Due to its unique characteristics, Cheuopodium quinoa has received wide attention in recent years. This paper elaborated the biological characteristics, nutritional value, utilization approach and economic benefits of Cheuopodium quinoa, introduced main problems in the development of Cheuopodium quinoa industry, and finally came up with recommendations for the development Cheuopodium quinoa industry in China

Study on Quinoa Characteristics in Central and Southern Hebei

Quinoa is rich in nutrients and has high economic value, which attracts people's attention. Given that the quinoa has not yet been planted in the low-altitude Central and Southern Hebei plain, we used the quinoa seeds from five different habitats in China to conduct a planting test under different date of seeding in the low-altitude Central and Southern Hebei plain, and obtained the comprehensive data about the phenophase and agronomic traits of quinoa in this region. The test results showed that the grain quinoa should not be planted in Central and Southern Hebei plain, the limiting factors were identified, and a new way for local development of vegetable quinoa was found

Interpreting Variety–Location–Fertilizer Interactions to Enhance Foxtail Millet Productivity in Northern China

Foxtail millet (Setaria italica (L.) P. Beauv.) is an important traditional cereal crop in dryland ecological agriculture in China and is widely grown in India, the United States, and Nigeria. It is of significance to understand the variety–location–fertilizer (V–L–F) interaction for highly efficient production. Therefore, a two-year field experiment was conducted with six varieties in five locations, and data were analyzed by combined ANOVA analysis, redundancy analysis (RDA), and additive main multiplicative interaction (AMMI). The results showed that the mean sum of squares was significantly different among years, locations, varieties, fertilizations, and their interactions, except for Y–V and V–F interactions. The contributions of various factors to yield variation varied, location was the largest contributor (38.7%), followed by year (33.6%), and variety and fertilizer contributed 7.1% and 3.2%, respectively. JI25 was widely adapted, and its yield was stable and higher than that of others over diverse environments in two years. The RDA results showed that two principal components explained more than 66.1% of the yield variance, while more than 63.0% of the variances were clustered in the first factor. Excessive single rainfall or total rainfall and air temperature (especially minimum temperature) were significantly associated with the millet yield. The results offered an important reference for variety layout, natural resource potential mining, and formulation of efficient green cultural practices

Interpreting Variety–Location–Fertilizer Interactions to Enhance Foxtail Millet Productivity in Northern China

Foxtail millet (Setaria italica (L.) P. Beauv.) is an important traditional cereal crop in dryland ecological agriculture in China and is widely grown in India, the United States, and Nigeria. It is of significance to understand the variety–location–fertilizer (V–L–F) interaction for highly efficient production. Therefore, a two-year field experiment was conducted with six varieties in five locations, and data were analyzed by combined ANOVA analysis, redundancy analysis (RDA), and additive main multiplicative interaction (AMMI). The results showed that the mean sum of squares was significantly different among years, locations, varieties, fertilizations, and their interactions, except for Y–V and V–F interactions. The contributions of various factors to yield variation varied, location was the largest contributor (38.7%), followed by year (33.6%), and variety and fertilizer contributed 7.1% and 3.2%, respectively. JI25 was widely adapted, and its yield was stable and higher than that of others over diverse environments in two years. The RDA results showed that two principal components explained more than 66.1% of the yield variance, while more than 63.0% of the variances were clustered in the first factor. Excessive single rainfall or total rainfall and air temperature (especially minimum temperature) were significantly associated with the millet yield. The results offered an important reference for variety layout, natural resource potential mining, and formulation of efficient green cultural practices