Leveraging Multimodal Fusion for Enhanced Diagnosis of Multiple Retinal Diseases in Ultra-wide OCTA

Ultra-wide optical coherence tomography angiography (UW-OCTA) is an emerging imaging technique that offers significant advantages over traditional OCTA by providing an exceptionally wide scanning range of up to 24 x 20 $mm^{2}$, covering both the anterior and posterior regions of the retina. However, the currently accessible UW-OCTA datasets suffer from limited comprehensive hierarchical information and corresponding disease annotations. To address this limitation, we have curated the pioneering M3OCTA dataset, which is the first multimodal (i.e., multilayer), multi-disease, and widest field-of-view UW-OCTA dataset. Furthermore, the effective utilization of multi-layer ultra-wide ocular vasculature information from UW-OCTA remains underdeveloped. To tackle this challenge, we propose the first cross-modal fusion framework that leverages multi-modal information for diagnosing multiple diseases. Through extensive experiments conducted on our openly available M3OCTA dataset, we demonstrate the effectiveness and superior performance of our method, both in fixed and varying modalities settings. The construction of the M3OCTA dataset, the first multimodal OCTA dataset encompassing multiple diseases, aims to advance research in the ophthalmic image analysis community

Genome-wide association studies reveal that members of bHLH subfamily 16 share a conserved function in regulating flag leaf angle in rice <i>(Oryza sativa)</i>

<div><p>As a major component of ideal plant architecture, leaf angle especially flag leaf angle (FLA) makes a large contribution to grain yield in rice. We utilized a worldwide germplasm collection to elucidate the genetic basis of FLA that would be helpful for molecular design breeding in rice. Genome-wide association studies (GWAS) identified a total of 40 and 32 QTLs for FLA in Wuhan and Hainan, respectively. Eight QTLs were commonly detected in both conditions. Of these, 2 and 3 QTLs were identified in the <i>indica</i> and <i>japonica</i> subpopulations, respectively. In addition, the candidates of 5 FLA QTLs were verified by haplotype-level association analysis. These results indicate diverse genetic bases for FLA between the <i>indica</i> and <i>japonica</i> subpopulations. Three candidates, <i>OsbHLH153</i>, <i>OsbHLH173</i> and <i>OsbHLH174</i>, quickly responded to BR and IAA involved in plant architecture except for <i>OsbHLH173</i>, whose expression level was too low to be detected; their overexpression in plants increased rice leaf angle. Together with previous studies, it was concluded that all 6 members in bHLH subfamily 16 had the conserved function in regulating FLA in rice. A comparison with our previous GWAS for tiller angle (TA) showed only one QTL had pleiotropic effects on FLA and TA, which explained low similarity of the genetic basis between FLA and TA. An ideal plant architecture is expected to be efficiently developed by combining favorable alleles for FLA from <i>indica</i> with favorable alleles for TA from <i>japonica</i> by inter-subspecies hybridization.</p></div

<i>OsbHLH174</i> overexpression transgenic plants showed an increase in the leaf angle.

<p>(A) The morphology of wild type (WT) and <i>OsbHLH174</i>: OX plants, at the seedling, tillering and heading stages. (B) quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) analysis of <i>OsbHLH174</i> transcripts in WT and <i>OsbHLH174</i>: OX at the seedling stage. (C) FLA and TSLA of the wild type and <i>OsbHLH174</i>:OX-1 and -2 at the heading stage (<i>P</i>< 0.001, n≥ 5).</p

Significant association loci for rice flag leaf angle detected in both Hainan and Wuhan using the linear mixed model in the germplasm collection.

<p>Significant association loci for rice flag leaf angle detected in both Hainan and Wuhan using the linear mixed model in the germplasm collection.</p

Summary of variances resolved by two-way analysis of variance for flag leaf angle in two environments.

<p>Summary of variances resolved by two-way analysis of variance for flag leaf angle in two environments.</p

Haplotype analysis of <i>qFLA1d</i>/<i>OsBRI1</i>.

<p>(A) Major haplotypes (haplotypes each carried by more than 5accessions) of <i>OsBRI1</i> in the full population according to SNPs data from RiceVarMap version 1. The SNPs in red and bold are non-Synonymous SNPs. (B) Comparison of FLA between Hap2 and Hap3 in <i>indica</i> rice by an independent <i>t</i>-test. (C) Comparison of FLA among Hap4-Hap6 in <i>japonica</i> rice by a Duncan’s test (<i>P</i>< 0.01), respectively.</p

Additional file 3: of Abnormal circadian oscillation of hippocampal MAPK activity and power spectrums in NF1 mutant mice

Supplementary tables were shown as the spike firing rates of pyramidal cells in mice. (ZIP 106 kb

Additional file 2: Figure S2. of Abnormal circadian oscillation of hippocampal MAPK activity and power spectrums in NF1 mutant mice

In vivo recording in CA1 demonstrates alterations in hippocampal rhythmic oscillations and firing rates in Nf1 +/− mice. a The local field potentials (LFPs) recordings in CA1(WT mice). First trace- unfiltered LFPs, second trace- alpha oscillations (filtered 7–12 Hz). b Histograms show the averaged power spectral density of the neuronal rhythmic oscillations (alpha). Data are expressed as mean ± SEM (WT, n = 5; Nf1 +/− , n = 5). Two-way analysis of variance with repeated measures and post hoc Bonferroni tests was used to evaluate differences in local field potential power spectrum density in day and night recordings in Nf1 +/− and WT groups. ***p < 0.001. (PDF 212 kb