A comparative analysis of a radiation-cooling-plate-coupled adhesion-jet air conditioning system in different positions

Compared with the traditional radiant cooling combined with a displacement ventilation air conditioning system, an air conditioning system of radiant cooling combined with an attached jet can not only effectively prevent dew on the surface of the radiant cooling plate, but also further improve the cooling capacity of the radiant air conditioning system; however, most scholars have installed the radiant cooling plate on the radiant roof and the ground, and there are fewer studies on installing the radiant cooling plate on the two sides of the wall. Based on this, this paper builds an experimental system of radiant air conditioning and conducts experiments on summer working conditions in June–October to experimentally study the indoor thermal and humid environments and thermal comfort under different water supply temperatures when radiant cold panels are installed in single-side-wall, symmetrical-wall, and top-panel positions. The experimental results show that the optimal water supply temperatures of single-side-wall radiation combined with an attached-jet air conditioning system, symmetrical-wall radiation combined with an attached-jet air conditioning system, and roof radiation combined with an attached-jet air conditioning system are 18 °C, 22 °C, and 16 °C, respectively, and at the same time, the temperatures of the human body’s working area under the above water supply temperatures are 26 °C, 26.3 °C, and 26.4 °C, respectively. The average humidities in the working area are 58%, 53%, and 57%, which can meet the requirements of our country’s level II comfort when the indoor heat and humidity environment is stable, the energy consumption amounts of the radiant end are 5.71 kW·h, 3.99 kW·h, and 10.81 kW·h, respectively, and the highest efficiency of cooling and dehumidification is achieved with the symmetric-wall radiation combined with the adherent-jet air conditioning system

Super-resolution enhancement of UAV images based on fractional calculus and POCS

A super-resolution enhancement algorithm was proposed based on the combination of fractional calculus and Projection onto Convex Sets (POCS) for unmanned aerial vehicles (UAVs) images. The representative problems of UAV images including motion blur, fisheye effect distortion, overexposed, and so on can be improved by the proposed algorithm. The fractional calculus operator is used to enhance the high-resolution and low-resolution reference frames for POCS. The affine transformation parameters between low-resolution images and reference frame are calculated by Scale Invariant Feature Transform (SIFT) for matching. The point spread function of POCS is simulated by a fractional integral filter instead of Gaussian filter for more clarity of texture and detail. The objective indices and subjective effect are compared between the proposed and other methods. The experimental results indicate that the proposed method outperforms other algorithms in most cases, especially in the structure and detail clarity of the reconstructed images

Dehydrocostus lactone inhibits the proliferation and metastasis of hepatocellular carcinoma cells via modulating p53-p21-CDK2 signaling pathway

Background: Hepatocellular carcinoma (HCC), a malignancy with high mortality and recurrence rates, has very limited options for treatment strategies. Dehydrocostus lactone (DL) is the principal quality marker extracted from Aucklandia lappa Decne. and has been demonstrated to possess excellent anticancer activity. The key purpose of this study was to explore the therapeutic effects and potential mechanisms of DL on HCC. Methods: The effect of DL on the cell viability of HCC cell lines (HepG2 and SK-HEP-1) and normal human hepatocyte cell line (L-O2) was examined by CCK8, colony formation, and BeyoClickTMEdU-488 assays; Hoechst 33,258 staining and flow cytometry were utilized to determine the impact of DL on apoptosis; the sensitivity of HCC cells to DL was explored by adding Z-VAD-fmk. In addition, the expression of apoptosis-related proteins Bax, Bcl-2, and PARP was detected by western blotting; the expression of p-H2AX was investigated by western blotting and immunofluorescence staining; cell cycle distribution was observed using flow cytometry; the migration ability and invasiveness of HCC cells were assessed by wound healing assay and transwell assay; immunoblotting was applied to visualize the levels of EMT markers in the two HCC cell lines. Transcriptome sequencing was performed to reveal the underlying mechanisms of DL anti-tumor; western blotting and qRT-PCR were employed to verify the mRNA and protein abundance of p53, p21, and CDK2; HepG2 xenograft in mice was used to test the anti-tumor effects of DL in vivo. Results: DL suppressed the proliferation of HCC cell lines (HepG2 and SK-HEP-1) but had little effect on a normal human liver cell line (L-O2). In addition, DL also induced apoptotic death of the HCC cells by activating Bax and downregulating Bcl-2. The antiproliferative effects of DL could be attributed to increased DNA damage and G1-phase cell cycle arrest. Moreover, DL inhibited HCC cell invasiveness and migration in vitro by decreasing the levels of β-catenin, N-cadherin, and TCF8/ZEB1, and increasing the E-cadherin level. RNA sequencing indicated that DL exerted its anti-hepatoma effects partly via regulation of p53-p21-CDK2 signaling. These results were validated by our in vivo experiments, in which DL markedly suppressed the growth of HepG2 xenografts in a mouse model without any toxic side effects, which corresponded to decreased expression of Ki67 and MMP9 in the tumor tissues. Conclusion: DL has significant anti-cancer effects in both in vivo and in vitro HCC models, and therefore could be further developed as a promising drug for the treatment of HCC

Integrated Solid-Phase Extraction, Ultra-High-Performance Liquid Chromatography–Quadrupole-Orbitrap High-Resolution Mass Spectrometry, and Multidimensional Data-Mining Techniques to Unravel the Metabolic Network of Dehydrocostus Lactone in Rats

Dehydrocostus lactone (DL) is among the representative ingredients of traditional Chinese medicine (TCM), with excellent anticancer, antibacterial, and anti-inflammatory activities. In this study, an advanced strategy based on ultra-high-performance liquid chromatography–quadrupole-Orbitrap high-resolution mass spectrometry (UHPLC–Q-Orbitrap HRMS) was integrated to comprehensively explore the metabolic fate of DL in rats. First, prior to data collection, all biological samples (plasma, urine, and feces) were concentrated and purified using solid-phase extraction (SPE) pre-treatment technology. Then, during data collection, in the full-scan (FS) data-dependent acquisition mode, FS-ddMS2 was intelligently combined with FS-parent ion list (PIL)-dynamic exclusion (DE) means for targeted monitoring and deeper capture of more low-abundance ions of interest. After data acquisition, data-mining techniques such as high-resolution extracted ion chromatograms (HREICs), multiple mass defect filters (MMDFs), diagnostic product ions (DPIs), and neutral loss fragments (NLFs) were incorporated to extensively screen and profile all the metabolites in multiple dimensions. As a result, a total of 71 metabolites of DL (parent drug included) were positively or tentatively identified. The results suggested that DL in vivo mainly underwent hydration, hydroxylation, dihydrodiolation, sulfonation, methylation, dehydrogenation, dehydration, N-acetylcysteine conjugation, cysteine conjugation, glutathione conjugation, glycine conjugation, taurine conjugation, etc. With these inferences, we successfully mapped the “stepwise radiation” metabolic network of DL in rats, where several drug metabolism clusters (DMCs) were discovered. In conclusion, not only did we provide a refined strategy for inhibiting matrix effects and fully screening major-to-trace metabolites, but also give substantial data reference for mechanism investigation, in vivo distribution visualization, and safety evaluation of DL