Overexpression of Pear (Pyrus pyrifolia) CAD2 in Tomato Affects Lignin Content

PpCAD2 was originally isolated from the ‘Wangkumbae’ pear (Pyrus pyrifolia Nakai), and it encodes for cinnamyl alcohol dehydrogenase (CAD), which is a key enzyme in the lignin biosynthesis pathway. In order to verify the function of PpCAD2, transgenic tomato (Solanum lycopersicum) ‘Micro-Tom’ plants were generated using over-expression constructs via the agrobacterium-mediated transformation method. The results showed that the PpCAD2 over-expression transgenic tomato plant had a strong growth vigor. Furthermore, these PpCAD2 over-expression transgenic tomato plants contained a higher lignin content and CAD enzymatic activity in the stem, leaf and fruit pericarp tissues, and formed a greater number of vessel elements in the stem and leaf vein, compared to wild type tomato plants. This study clearly indicated that overexpressing PpCAD2 increased the lignin deposition of transgenic tomato plants, and thus validated the function of PpCAD2 in lignin biosynthesis

PpNAC187 Enhances Lignin Synthesis in ‘Whangkeumbae’ Pear (Pyrus pyrifolia) ‘Hard-End’ Fruit

A disorder in pears that is known as ‘hard-end’ fruit affects the appearance, edible quality, and market value of pear fruit. RNA-Seq was carried out on the calyx end of ‘Whangkeumbae’ pear fruit with and without the hard-end symptom to explore the mechanism underlying the formation of hard-end. The results indicated that the genes in the phenylpropanoid pathway affecting lignification were up-regulated in hard-end fruit. An analysis of differentially expressed genes (DEGs) identified three NAC transcription factors, and RT-qPCR analysis of PpNAC138, PpNAC186, and PpNAC187 confirmed that PpNAC187 gene expression was correlated with the hard-end disorder in pear fruit. A transient increase in PpNAC187 was observed in the calyx end of ‘Whangkeumbae’ fruit when they began to exhibit hard-end symptom. Concomitantly, the higher level of PpCCR and PpCOMT transcripts was observed, which are the key genes in lignin biosynthesis. Notably, lignin content in the stem and leaf tissues of transgenic tobacco overexpressing PpNAC187 was significantly higher than in the control plants that were transformed with an empty vector. Furthermore, transgenic tobacco overexpressing PpNAC187 had a larger number of xylem vessel elements. The results of this study confirmed that PpNAC187 functions in inducing lignification in pear fruit during the development of the hard-end disorder. View Full-Tex

FROM EAST TO WEST: DOES ENCULTURATION CHANGE HOW PEOPLE CONCEPTUALIZE EVENTS

25 pagesMinds differ between East Asians and Westerners. According to an influential claim in Cultural Psychology, Westerners think more abstractly than East Asians. Contrary to this claim, however, our previous studies showed that Chinese individuals in China tended to construe events more abstractly than Caucasian individuals in the United States, as measured by a validated measure of abstract vs. concrete thinking called the Behavioral Identification Form (BIF; Vallacher & Wegner, 1989). Here we tested whether Chinese individuals who spend time in the United States construe events more concretely, like Westerners do. We administered the BIF to 198 Chinese participants attending US colleges. Chinese participants who have been living in the US construed events significantly more concretely than the Chinese individuals who were tested previously in China, and participants who had lived in the US for a longer time construed events more concretely than participants who had lived in the US for a shorter time. Although these data are correlational, they are consistent with an effect of Western enculturation on Eastern minds.2022-05-3

PpNAC187 Enhances Lignin Synthesis in ‘Whangkeumbae’ Pear (Pyrus pyrifolia) ‘Hard-End’ Fruit

A disorder in pears that is known as ‘hard-end’ fruit affects the appearance, edible quality, and market value of pear fruit. RNA-Seq was carried out on the calyx end of ‘Whangkeumbae’ pear fruit with and without the hard-end symptom to explore the mechanism underlying the formation of hard-end. The results indicated that the genes in the phenylpropanoid pathway affecting lignification were up-regulated in hard-end fruit. An analysis of differentially expressed genes (DEGs) identified three NAC transcription factors, and RT-qPCR analysis of PpNAC138, PpNAC186, and PpNAC187 confirmed that PpNAC187 gene expression was correlated with the hard-end disorder in pear fruit. A transient increase in PpNAC187 was observed in the calyx end of ‘Whangkeumbae’ fruit when they began to exhibit hard-end symptom. Concomitantly, the higher level of PpCCR and PpCOMT transcripts was observed, which are the key genes in lignin biosynthesis. Notably, lignin content in the stem and leaf tissues of transgenic tobacco overexpressing PpNAC187 was significantly higher than in the control plants that were transformed with an empty vector. Furthermore, transgenic tobacco overexpressing PpNAC187 had a larger number of xylem vessel elements. The results of this study confirmed that PpNAC187 functions in inducing lignification in pear fruit during the development of the hard-end disorder

Patients with liver FNH and HCC patients with negative AFP: plain and dynamic enhanced MRI and CT findings

ObjectiveTo investigate plain and dynamic enhanced magnetic resonance imaging (MRI) and computed tomography (CT) findings in patients with focal nodular hyperplasia (FNH) of the liver and hepatocellular carcinoma (HCC) patients with negative alpha-fetoprotein (AFP). MethodsA statistical analysis was performed on the clinical data of 124 cases of liver tumor admitted to Beijing Miyun County Hospital from April 2012 to April 2014. ResultsFifty-five of the 74 patients with FNH underwent CT examination, among whom 38 patients received three-phase dynamic enhanced scan and 16 received only plain scan; 62 cases had plain and enhanced MRI with the application of contrast agent Gd-BOPTA in 42 patients. Among the 50 HCC patients with negative AFP, CT examination was performed in 40 and 10 only had plain scan; 46 patients received plain and enhanced MRI with the use of contrast agent Gd-BOPTA in 30. Delayed scan after 1-2 h demonstrated low signal in 30 lesions of the 30 cases. ConclusionFor patients with liver FNH and AFP-negative HCC patients, their plain and dynamic enhanced MRI and CT scan have respective characteristics. A combination of multiple examination methods can significantly improve diagnostic yield of the two diseases

Singing-Voice Timbre Evaluations Based on Transfer Learning

The development of artificial intelligence technology has made it possible to realize automatic evaluation systems for singing, and relevant research has been able to achieve accurate evaluations with respect to pitch and rhythm, but research on singing-voice timbre evaluation has remained at the level of theoretical analysis. Timbre is closely related to expression performance, breath control, emotional rendering, and other aspects of singing skills, and it has a crucial impact on the evaluation of song interpretation. The purpose of this research is to investigate the automatic evaluation method of singing-voice timbre. At the present stage, timbre research generally has problems such as a paucity of datasets, a single evaluation index, easy overfitting or a model’s failure to converge. Compared with the singing voice, the research on musical instruments is more mature, with more available data and richer evaluation dimensions. We constructed a deep network based on the CRNN model to perform timbre evaluation, and the test results showed that cross-media learning of timbre evaluation is feasible, which also indicates that humans have a consistent timbre perception with respect to musical instruments and vocals

Is adults’ ability to interpret iconicity shared between the spoken and gestural modalities?

Iconicity (the resemblance between form and meaning) exists in various modes of communication. This study investigated whether adults interpret iconicity in speech and gesture via a modality-independent ability. We tested 348 adult participants and assessed their ability to use iconic prosody and iconic gesture cues when interpreting novel verb meanings. We manipulated the rate of the spoken novel verbs (iconic prosody) and the rate of observed hand movements (iconic gestures) to be either fast or slow in two verb-action matching tasks. Adults could use these iconic speed cues to interpret novel verbs as referring to a fast or slow version of the same action. Adults showed similar performances in the two verb-action matching tasks: those who performed well in the iconic prosody task also performed well in the iconic gesture task. This positive correlation persisted even after controlling verbal working memory. Thus, we conclude that adults possess a modality-independent ability for interpreting iconicity