Genome editing in kenaf: Initial studies and target gene characterization

The potential for Hibiscus cannabinus L. (kenaf) improvement via genome editing using the CRISPR/Cas9 system to generate gene knock-outs was explored. Studies included target gene identification, target guide RNA (gRNA) selection, plant tissue (explant) choice and media composition for plant regeneration. A putative kenaf phytoene desaturase gene (pds, GEED01047592.1) was identified in the kenaf transcriptome, and molecularly confirmed. Kenaf seedling tissues were transformed via Agrobacterium tumefaciens containing the cas9 gene (endonuclease required for gene knock-out) and each gRNA separately; putative transgenic calli and adventitious shoots arose on a medium containing 1-naphthaleneacetic acid, thidiazuron and silver nitrate. Tissues appeared chlorotic/albino and shoots remained diminutive/dwarf-like. These unique morphologies had also been noted by researchers who successfully knocked out the pds gene in other plant species. Cas9 DNA was detected in these putative transgenic kenaf tissues, but initial DNA sequencing analysis did not confirm knock-out/mutations in targeted areas of the pds gene

Towards a compact soliton microcomb fully referenced on atomic reference

A fully stabilized soliton microcomb is critical for many applications of optical frequency comb based on microresonators. However, the current approaches for full frequency stabilization require either external acousto-optic or electro-optic devices or auxiliary lasers and multiple phase-locked loops, which compromises the convenience of the system. This study explores a compact atomic referenced fully stabilized soliton microcomb that directly uses a rubidium atomic optical frequency reference as the pump source, and complements the repetition rate (7.3 GHz) of the soliton microcomb was phase-locked to an atomic-clock-stabilized radio frequency (RF) reference by mechanically tuning the resonance of the optical resonator. The results demonstrate that the stability of the comb line (0.66 THz away from the pump line) is consistent with that of the Rb87 optical reference, attaining a level of approximately 4 Hz @100 s, corresponding to the frequency stability of 2E-14 @100 s. Furthermore,the frequency reproducibility of the comb line was evaluated over six days and it was discovered that the standard deviation (SD) of the frequency of the comb line is 10 kHz, resulting in a corresponding absolute deviation uncertainty of 1.3E-10, which is technically limited by the locking range of the soliton repetition rate. The proposed method gives a low-power and compact solution for fully stabilized soliton micorcombs.Comment: 6 pages, 5 figure

Soliton generation in CaF2_2 crystalline whispering gallery mode resonators with negative thermal-optical effects

Calcium fluoride (CaF$_2$) crystalline whispering gallery mode resonators (WGMRs) exhibit ultrahigh intrinsic quality factors and a low power anomalous dispersion in the communication and mid-infrared bands, making them attractive platforms for microresonator-based comb generation. However, their unique negative thermo-optic effects pose challenges when achieving thermal equilibrium. To our knowledge, our experiments serve as the first demonstration of soliton microcombs in Q > 109 CaF$_2$ WGMRs. We observed soliton mode-locking and bidirectional switching of soliton numbers caused by the negative thermo-optic effects. Additionally, various soliton formation dynamics are shown, including breathing and vibrational solitons, which can be attributed to thermo-photomechanical oscillations. Thus, our results enrich the soliton generation platform and provide a reference for generating solitons from WGMRs that comprise other materials with negative thermo-optic effects. In the future, the ultrahigh quality factor of CaF$_2$ crystal cavities may enable the generation of sub-milliwatt-level broad-spectrum soliton combs.Comment: 4 pages,5 pictures,description of soliton generation in a calcium fluoride whisper gallery mode microresonators with negative thermo-optical effect,ready for publication in optics lette

How to Survive between "Standardized Resident Training " and "Professional Master" -On the Difficulties Encountered in Undergraduate Clinical Practice

To review on the difficulties encountered by medical bachelor students for their career development after graduation, and to explore potential solutions to their current situation, thus provide them possibilities of making good use of professional training and skills acquired in campus

A Disposable Pneumatic Microgripper for Cell Manipulation with Image-Based Force Sensing

A new design for a single-use disposable pneumatic microgripper is presented in this paper. It enables very cost-eective batch microfabrication in SU-8 with a single lithography mask by shifting manufacturing complexity into reusable components. An optically readable force sensor with potential to be used in a feedback loop has been integrated in order to enable gripping with a controlled force. The sensors are first examined separately from the gripper and exhibit good linearity. The gripper function utilizes the disposable gripper element together with a reusable gripper fixture. During experiments, the pneumatically actuated microgripper can vary the gripping force within a range of a few mN (up to 5.7 mN was observed). This microgripper is planned to be used in a liquid environment for gripping larger aggregates of cells in combination with the patch clamp technique. This approach will allow Langerhans islets suspended in an electrolyte solution to be grasped and held during electrophysiological measurements without cell damage

A suggested approach to the diagnosis of urinary tract infection and empiric use of antibiotics among hospitalized older adults with acute confusion and or underlying cognitive and communication deficits

BackgroundThe true incidence of urinary tract infection (UTI) among hospitalized older adults remains unknown.AimsWe aim to present an approach to improve correct identification of UTI diagnosis and empiric use of antibiotics among those who are unable to provide history.Methods A comprehensive review of the literature was undertaken.Results Our suggested approach appears safe and likely to enhance diagnostic accuracy and judicious use of antibiotics among hospitalized older adults.ConclusionOur suggested approach is based on best available literature evidence but requires validation in clinical studies

Effects of mulberry twig alkaloids(Sangzhi alkaloids) and metformin on blood glucose fluctuations in combination with premixed insulin-treated patients with type 2 diabetes

IntroductionWe aimed to evaluated the effect of premixed insulin (Ins), premixed insulin combined with metformin (Ins+Met) or mulberry twig alkaloids(Ins+SZ-A) on blood glucose fluctuations in patients with type 2 diabetes (T2DM) using continuous glucose monitors (CGM).MethodsThirty patients with T2DM and poor blood glucose control using drugs were evaluated for eligibility during the screening period. Subsequently, their original hypoglycemic drugs were discontinued during the lead-in period, and after receiving Ins intensive treatment for 2 weeks, they were randomly assigned to receive either Ins, Ins+Met, or Ins+SZ-A treatment for the following 12 weeks. The main efficacy endpoint comprised changes in their CGM indicators changes (mean blood glucose level [MBG], standard deviation of blood glucose [SDBG], mean amplitude of glycemic excursions [MAGE], postprandial glucose excursions [PPGE], the largest amplitude of glycemic excursions [LAGE], mean of daily difference [MODD], time in range between 3.9–10.0 mmol/L [TIR] and area under the curve for each meal [AUCpp]) during the screening, lead-in, and after 12-week treatment period. Changes in glycosylated hemoglobin (HbA1c), fasting blood glucose (FBG), 1-h postprandial blood glucose (1h-PBG), 2-h postprandial blood glucose (2h-PBG), fasting blood lipids and postprandial blood lipids were also measured at baseline and after 12 weeks of treatmentResultsThe CGM indicators of the three groups during the lead-in period all showed significant improvements compared to the screening period (P<0.05). Compared with those in the lead-in period, all of the CGM indicators improved in the the Ins+Met and Ins+SZ-A groups after 12 weeks of treatment (P<0.05), except for MODD. After 12-week treatment, compared with the Ins group, Ins+Met and Ins+SZ-A groups showed improved MBG, SDBG, TIR, breakfast AUCpp,lunch AUCpp, HbA1c, FBG, 1h-PBG, fasting blood lipid and postprandial blood lipid indicators (P<0.05). Further, the LAGE, PPGE, MAGE, dinner AUCpp and 2h-PBG levels of the Ins+SZ-A group were significantly lower than those of the Ins+Met and Ins groups (P<0.05).ConclusionOur findings highlight the efficacy of combination therapy (Ins+SZ-A or Ins+Met) in improving blood glucose fluctuations, as well as blood glucose and lipid levels. Ins+SZ-A reduces postprandial blood glucose fluctuations more than Ins+Met and Ins groups.Trial registration numberISRCTN20835488

Bodily Manipulation of the Expressed Dominance on a Humanoid Robot

The subject of this thesis is to find a way to manipulate the bodily dominant / submissive expressions of robotic behaviors. The expression of emotion is an important part of the development of Socially Interactive Robots. Previously, studies about the robotic emotions usually focus on single behaviors. Because the generation and testing of new behaviors are time consuming, this kind of emotional manipulation procedure requires a lot of work when applied in practical use. The innovation of this thesis is to explore a solution that can modify the dominance level of a wide range of robotic behaviors without the re-creation of new behaviors.The results of this work can be used for the design of the robotic movements and the development of robotic applications

Highlights to phytosterols accumulation and equilibrium in plants: Biosynthetic pathway and feedback regulation

Phytosterols are a group of sterols exclusive to plants and fungi, but are indispensable to humans because of their medicinal and nutritional values. However, current raw materials used for phytosterols extraction add to the cost and waste in the process. For higher sterols production, major attention is drawn to plant materials abundant in phytosterols and genetic modification. To provide an insight into phytosterols metabolism, the research progress on key enzymes involved in phytosterols biosynthesis and conversions were summarized. CAS, SSR2, SMT, DWFI and CYP710A, the enzymes participating in the biosynthetic pathway, and PSAT, ASAT and SGT, the enzymes involved in the conversion of free sterols to conjugated ones, were reviewed. Specifically, SMT and CYP710A were emphasized for their function on modulating the percentage composition of different kinds of phytosterols. The thresholds of sterol equilibrium and the resultant phytosterols accumulation, which vary in plant species and contribute to plasma membrane remodeling under stresses, were also discussed. By retrospective analysis of the previous researches, we proposed a feedback mechanism regulating sterol equilibrium underlying sterols metabolism. From a strategic perspective, we regard salt tolerant plant as an alternative to present raw materials, which will attain higher phytosterols production in combination with gene-modification

Evolution of Volatile Aroma Compounds and Amino Acids in Cabernet Gernischt Grape Berries (Vitis vinifera L.): Comparison of Different Training Systems for Mechanical Soil Burial

Soil burial is a survival strategy for grapevines that can safely overwinter in north-western regions of China. A suitable training system was beneficial for soil burial to provide winter protection. Moreover, the training system can also significantly affect fruit quality during the development of grape berries, such as primary and secondary metabolites. In this study, four-year-old Cabernet Gernischt grapevines were used as experimental material and exposed to four training systems, including the Ningxia traditional vertical trunk (control, CK); the gobelet (T1); single guyot (T2); slant trunk with vertical shoot positioning (STVSP) (T3). The results showed that total soluble solid total phenol content was 12.69%, 57% higher under T3 training systems than in the control, and T3 alleviated the canopy density, leading to improving the leaf photosynthetic efficiency gas chromatography-mass spectrometry (GC-MS) assay used to detect the aroma compounds. The results indicated that the T3 training system enhanced the accumulation of alcohols, carbonyl compounds, C6/C9 and esters, which account for the largest proportion of volatile compounds, and the qRT-PCR reveals that VvEcar, Vvter, VvCCD1, and VvLis were raised under T3 at the transcriptional level. Moreover, T3 contributes to most free amino acid synthesis. Additionally, the PCA reveals the correlation of free amino acids under four training systems, which reflected the mostly amino acid related to T3, and thus, we could speculate that T3 enhances the overall aroma. These results may lead to new strategies to select a new, short trunk training system to achieve mechanized buried soil, to prevent cold and produce high-quality wine in this area