A Preliminary Stratigraphic Study of the Galena Group of Winneshiek County, Iowa

The rocks of the Galena Group have proved to be problematic in correlation. This problem is due to a variable lithofacies development. A preliminary investigation, although based upon exposures studied just within Winneshiek County, provides a method for field identification in Iowa of stratigraphic units currently used by the Illinois State Geological Survey within Illinois. Units under consideration at this time are restricted to the Dunleith, Wise Lake, and Dubuque Formations. Methods of identification and correlation employ in part such physical features as sequences of nodular chart bands, discontinuity surfaces, and sparry calcarenite bands. Investigation has disclosed a close relationship of these three features. A composite graphic column is provided, with key identification factors, for each of the subdivisions together with reference localities where the strata under investigation may be observed in sequence

Unusual Beach Deposits in Oolite Carbonate Environments Mississippian and Recent

Thousands of small fossils were collected from unusual lenses within the Cyathophyllum Zone, Gilmore City Limestone (Mississippian) in a large quarry near Humboldt, Iowa. These rare lenses occur in an interval 1-2.5m thick that shows an extreme variability of facies. An intensive search of other Gilmore City outcrops revealed no similar lenses. The rest of the interval, outside the lenses, contained larger fossils and fossil fragments. The small fossils in the lenses are remarkably well preserved, the gastropods particularly so. Comparison of the Gilmore City Limestone with Recent oolitic deposits at Paradise Island in the Bahamas leads to the conclusion that these unusual lenses probably were a backshore deposit. The Bahamian deposits provide evidence for interpretation of these fossils as size-sorted rather than dwarfed. Early carbonate coating is hypothesized to explain the exceptional preservation of the Mississippian fossils

Direct and indirect co-culture of chondrocytes and mesenchymal stem cells for the generation of polymer/extracellular matrix hybrid constructs

In this work, the influence of direct cell–cell contact in co-cultures of mesenchymal stem cells (MSCs) and chondrocytes for the improved deposition of cartilage-like extracellular matrix (ECM) within nonwoven fibrous poly(∊-caprolactone) (PCL) scaffolds was examined. To this end, chondrocytes and MSCs were either co-cultured in direct contact by mixing on a single PCL scaffold or produced via indirect co-culture, whereby the two cell types were seeded on separate scaffolds which were then cultured together in the same system either statically or under media perfusion in a bioreactor. In static cultures, the chondrocyte scaffold of an indirectly co-cultured group generated significantly greater amounts of glycosaminoglycan and collagen than the direct co-culture group initially seeded with the same number of chondrocytes. Furthermore, improved ECM production was linked to greater cellular proliferation and distribution throughout the scaffold in static culture. In perfusion cultures, flow had a significant effect on the proliferation of the chondrocytes. The ECM contents within the chondrocyte-containing scaffolds of the indirect co-culture groups either approximated or surpassed the amounts generated within the direct co-culture group. Additionally, within bioreactor culture there were indications that chondrocytes had an influence on the chondrogenesis of MSCs as evidenced by increases in cartilaginous ECM synthetic capacity. This work demonstrates that it is possible to generate PCL/ECM hybrid scaffolds for cartilage regeneration by utilizing the factors secreted by two different cell types, chondrocytes and MSCs, even in the absence of juxtacrine signaling

Stratigraphy of the Dubuque Formation (Upper Ordovician) in Iowa

The Dubuque Formation of Upper Ordovician age crops out in the Upper Mississippi Valley. It comprises interbedded carbonate and argillaceous rocks that are approximately 35 feet thick in Iowa and Illinois, but thicken to a maximum of approximately 45 feet in southern Minnesota. Three proposed informal subdivisions: Frankville, Luana, and Littleport beds, are differentiated on the basis of bed surface topography ranging upward from nearly planar beds in the Frankville to prominently undulose surfaces in the Littleport beds. The Frankville beds represent a transition from the massive dolomite of the underlying Stewartville Member of the Wise Lake Formation to the overlying interbedded carbonate rocks and shale of the upper Dubuque. The base of the Dubuque Formation in Iowa and Minnesota is placed at a prominent, approximately 8 inch thick, carbonate bed at the base of the Frankville beds. This \u27\u27marker bed\u27\u27 provides a more precise datum for lithostratigraphic correlation than the lowest prominent shale parting employed by previous workers to identify the base of the Dubuque

Fabrication and characterization of multiscale electrospun scaffolds for cartilage regeneration

Recently, scaffolds for tissue regeneration purposes have been observed to utilize nanoscale features in an effort to reap the cellular benefits of scaffold features resembling extracellular matrix (ECM) components. However, one complication surrounding electrospun nanofibers is limited cellular infiltration. One method to ameliorate this negative effect is by incorporating nanofibers into microfibrous scaffolds. This study shows that it is feasible to fabricate electrospun scaffolds containing two differently scaled fibers interspersed evenly throughout the entire construct as well as scaffolds containing fibers composed of two discrete materials, specifically fibrin and poly(?-caprolactone). In order to accomplish this, multiscale fibrous scaffolds of different compositions were generated using a dual extrusion electrospinning setup with a rotating mandrel. These scaffolds were then characterized for fiber diameter, porosity and pore size and seeded with human mesenchymal stem cells to assess the influence of scaffold architecture and composition on cellular responses as determined by cellularity, histology and glycosaminoglycan (GAG) content. Analysis revealed that nanofibers within a microfiber mesh function to maintain scaffold cellularity under serum-free conditions as well as aid the deposition of GAGs. This supports the hypothesis that scaffolds with constituents more closely resembling native ECM components may be beneficial for cartilage regeneration