The Mental Health Impact of Intensive Mothering Ideology on Contemporary Mothers

This research explored how exposure to Intensive Mothering Ideology (IMI) may impact maternal mental health in modern mothers. Susan Hays coined the IMI term in 1996, inspiring a significant body of research around the topic which has shown that mothers generally process intensive mothering discourse contextually with consideration for unique era-specific societal challenges (Constantinou, Varela, & Buckby, 2021; Hays, 1996). To this author’s knowledge, there have been no general examinations of the impact of IMI on maternal mental health since before the outbreak of COVID-19 (though there have been a few pointed examinations of IMI in relation to the pandemic). This research aims to understand mothers’ experiences around intensive mothering discourse during the summer of 2022 (when seventeen semi-structured interviews were conducted) with respect to the unique social context of that time, how IMI content was consumed, processed, and internalized, and to what extent their experiences may have impacted their psychological wellbeing. Using the Auerbach and Silverstein (2003) method, interview transcripts were methodically coded and analyzed to uncover relevant text, repeating ideas (62 total), themes (14 total), and theoretical constructs (six total which were validated through study member checks) (Auerbach & Silverstein, 2003). Results showcase participant reflections on the roles that make up their identities, their values around mothering including external support systems, the pressures they feel to mother in a certain way, how they conceptualize the “ideal” mother, their relationships to social media, their experiences mothering during COVID-19, and their mental and physical wellbeing. Discussion contextualizes results within intersecting theories and sews the data together in light of these theories to craft a narrative that meaningfully represents participant experiences

Evaluating and optimizing oral formulations of live bacterial vaccines using a gastro-small intestine model

Gastrointestinal (GI) models that mimic physiological conditions in vitro are important tools for developing and optimizing biopharmaceutical formulations. Oral administration of live attenuated bacterial vaccines (LBV) can safely and effectively promote mucosal immunity but new formulations are required that provide controlled release of optimal numbers of viable bacterial cells, which must survive gastrointestinal transit overcoming various antimicrobial barriers. Here, we use a gastro-small intestine gut model of human GI conditions to study the survival and release kinetics of two oral LBV formulations: the licensed typhoid fever vaccine Vivotif comprising enteric coated capsules; and an experimental formulation of the model vaccine Salmonella Typhimurium SL3261 dried directly onto cast enteric polymer films and laminated to form a polymer film laminate (PFL). Neither formulation released significant numbers of viable cells when tested in the complete gastro-small intestine model. The poor performance in delivering viable cells could be attributed to a combination of acid and bile toxicity plus incomplete release of cells for Vivotif capsules, and to bile toxicity alone for PFL. To achieve effective protection from intestinal bile in addition to effective acid resistance, bile adsorbent resins were incorporated into the PFL to produce a new formulation, termed BR-PFL. Efficient and complete release of 4.4x107 live cells per dose was achieved from BR-PFL at distal intestinal pH, with release kinetics controlled by the composition of the enteric polymer film, and no loss in viability observed in any stage of the GI model. Use of this in vitro GI model thereby allowed rational design of an oral LBV formulation to maximize viable cell release

Atom trapping and two-dimensional Bose-Einstein condensates in field-induced adiabatic potentials

We discuss a method to create two-dimensional traps as well as atomic shell, or bubble, states for a Bose-Einstein condensate initially prepared in a conventional magnetic trap. The scheme relies on the use of time-dependent, radio frequency-induced adiabatic potentials. These are shown to form a versatile and robust tool to generate novel trapping potentials. Our shell states take the form of thin, highly stable matter-wave bubbles and can serve as stepping-stones to prepare atoms in highly-excited trap eigenstates or to study `collapse and revival phenomena'. Their creation requires gravitational effects to be compensated by applying additional optical dipole potentials. However, in our scheme gravitation can also be exploited to provide a route to two-dimensional atom trapping. We demonstrate the loading process for such a trap and examine experimental conditions under which a 2D condensate may be prepared.Comment: 16 pages, 10 figure

Enteropathogenic Escherichia coli (EPEC) inactivate innate immune responses prior to compromising epithelial barrier function

Enteropathogenic Escherichia coli (EPEC) infection of the human small intestine induces severe watery diarrhoea linked to a rather weak inflammatory response despite EPEC's in vivo capacity to disrupt epithelial barrier function. Here, we demonstrate that EPEC flagellin triggers the secretion of the pro-inflammatory cytokine, interleukin (IL)-8, from small (Caco-2) and large (T84) intestinal epithelia model systems. Interestingly, IL-8 secretion required basolateral infection of T84 cells implying that flagellin must penetrate the epithelial barrier. In contrast, apical infection of Caco-2 cells induced IL-8 secretion but less potently than basolateral infections. Importantly, infection of Caco-2, but not T84 cells rapidly inhibited IL-8 secretion by a mechanism dependent on the delivery of effectors through a translocation system encoded on the locus of enterocyte effacement (LEE). Moreover, EPEC prevents the phosphorylation-associated activation of multiple kinase pathways regulating IL-8 gene transcription by a mechanism apparently independent of LEE-encoded effectors and four non-LEE-encoded effectors. Crucially, our studies reveal that EPEC inhibits the capacity of the cells to secrete IL-8 in response to bacterial antigens and inflammatory cytokines prior to disrupting barrier function by a distinct mechanism. Thus, these findings also lend themselves to a plausible mechanism to explain the absence of a strong inflammatory response in EPEC-infected humans

A laminated polymer film formulation for enteric delivery of live vaccine and probiotic bacteria

Live bacterial cells (LBC) are administered orally as attenuated vaccines, to deliver biopharmaceutical agents, and as probiotics to improve gastrointestinal health. However, LBC present unique formulation challenges and must survive gastrointestinal antimicrobial defenses including gastric acid after administration. We present a simple new formulation concept, termed Polymer Film Laminate (PFL). LBC are ambient dried onto cast acid-resistant enteric polymer films that are then laminated together to produce a solid oral dosage form. LBC of a model live bacterial vaccine and a probiotic were dried directly onto a cast film of enteric polymer. The effectiveness at protecting dried cells in a simulated gastric fluid (pH 2.0) depended on the composition of enteric polymer film used, with a blend of ethylcellulose plus Eudragit L100 55 providing greater protection from acid than Eudragit alone. However, although PFL made from blended polymers films completely released low molecular weight dye into intestinal conditions (pH 7.0), they failed to release LBC. In contrast, PFL made from Eudragit alone successfully protected dried probiotic or vaccine LBC from simulated gastric fluid for 2h, and subsequently released all viable cells within 60min of transfer into simulated intestinal fluid. Release kinetics could be controlled by modifying the lamination method

On the Spiral Structure of the Milky Way Galaxy

We consider the possible pattern of the overall spiral structure of the Galaxy, using data on the distribution of neutral (atomic), molecular, and ionized hydrogen, on the base of the hypothesis of the spiral structure being symmetric, i.e. the assumption that spiral arms are translated into each other for a rotation around the galactic center by 180{\deg} (a two-arm pattern) or by 90{\deg} (a four-arm pattern). We demonstrate that, for the inner region, the observations are best represented with a four-arm scheme of the spiral pattern, associated with all-Galaxy spiral density waves. The basic position is that of the Carina arm, reliably determined from distances to HII regions and from HI and H2 radial velocities. This pattern is continued in the quadrants III and IV with weak outer HI arms; from their morphology, the Galaxy should be considered an asymmetric multi-arm spiral. The kneed shape of the outer arms that consist of straight segments can indicate that these arms are transient formations that appeared due to a gravitational instability in the gas disk. The distances between HI superclouds in the two arms that are the brightest in neutral hydrogen, the Carina arm and the Cygnus (Outer) arm, concentrate to two values, permitting to assume the presence of a regular magnetic field in these arms.Comment: 21 pages, 14 fugures; accepted for publication in Astronomichesky Journal (Astron. Rep.

Parkin-deficient Mice Exhibit Nigrostriatal Deficits but not Loss of Dopaminergic Neurons

Loss-of-function mutations in parkin are the major cause of early-onset familial Parkinson's disease. To investigate the pathogenic mechanism by which loss of parkin function causes Parkinson's disease, we generated a mouse model bearing a germline disruption in parkin. Parkin-/- mice are viable and exhibit grossly normal brain morphology. Quantitative in vivo microdialysis revealed an increase in extracellular dopamine concentration in the striatum of parkin-/- mice. Intracellular recordings of medium-sized striatal spiny neurons showed that greater currents are required to induce synaptic responses, suggesting a reduction in synaptic excitability in the absence of parkin. Furthermore, parkin-/- mice exhibit deficits in behavioral paradigms sensitive to dysfunction of the nigrostriatal pathway. The number of dopaminergic neurons in the substantia nigra of parkin-/- mice, however, is normal up to the age of 24 months, in contrast to the substantial loss of nigral neurons characteristic of Parkinson's disease. Steady-state levels of CDCrel-1, synphilin-1, and α-synuclein, which were identified previously as substrates of the E3 ubiquitin ligase activity of parkin, are unaltered in parkin-/- brains. Together these findings provide the first evidence for a novel role of parkin in dopamine regulation and nigrostriatal function, and a non-essential role of parkin in the survival of nigral neurons in mice

Perioperative Medication Errors Building Safer Systems

I n this month's issue of Anesthesiology, nanji and coworkers 1 present a landmark study that should stimulate discussion and prompt improvements in medication safety in the operating room. the authors performed a prospective, observational clinical trial in a tertiary-care teaching hospital, which measured the frequency of medication errors and adverse drug events during the perioperative period. they reported the numbers of errors and adverse events as percentages of the total number of medications administered. in addition, a retrospective chart review was performed to capture medication errors and adverse events that were missed during the observation period. on the basis of their results, the authors developed recommendations that they believe would have prevented the particular errors and adverse events that were observed during the study. A total of 277 operations were observed, during which a total of 3,671 medications were administered. About 1 in every 20 medications administered involved a drug error and/or an adverse drug event. specifically, a total of 153 medication errors occurred, about one third of which caused an observable adverse drug event (33.3%). A total of 193 medication errors and/or adverse drug events were observed, of which 153 (79%) were preventable and 40 (21%) were not preventable. no medication-related deaths were observed; however, 133 (68.9%) of the observed or potential adverse drug events were serious and 3 (1.6%) were life-threatening. The single most common type of error was a labeling error (37 events; 24.2%). There was no difference in event rates for patients who underwent general anesthesia (227 cases, 82.0% of the total, 3,297 medications administered, 5.3% event rate) and those who underwent sedation only (37 cases, 13.4% of the total, 374 medications administered, 4.6% event rate). one third of the anesthesia care providers were house staff (n = 93, 33.6%); however, no differences in event rates were observed among house staff (68 events, 5.1% event rate), nurse anesthetists (111 events, 5.5% event rate), and staff anesthesiologists (14 events, 4.5% event rate). The high error and adverse event rates reported by nanji and coworkers are surprising and raise several important questions. Why were the rates of medication events substantially higher than those reported in previous studies? 2,3 Did observers include trivial events or events that simply reflected a difference in opinion (e.g., choice of drug dose)? Despite the uncertainties, several attributes of the study suggest the high event rates are accurate. First, events were detected by direct third-party observation rather than by self-reporting or facilitated incident reporting (e.g., where reports are completed whether a drug error has occurred). The incidence of errors is typically much higher when events are detected by impartial observers rather than through selfreporting or surveys. 4,5 second, the observers who detected the events were fully trained, experienced anesthesia care providers, not less experienced research personnel. Third

A Survey on the Krein-von Neumann Extension, the corresponding Abstract Buckling Problem, and Weyl-Type Spectral Asymptotics for Perturbed Krein Laplacians in Nonsmooth Domains

In the first (and abstract) part of this survey we prove the unitary equivalence of the inverse of the Krein--von Neumann extension (on the orthogonal complement of its kernel) of a densely defined, closed, strictly positive operator, $S\geq \varepsilon I_{\mathcal{H}}$ for some $\varepsilon >0$ in a Hilbert space $\mathcal{H}$ to an abstract buckling problem operator. This establishes the Krein extension as a natural object in elasticity theory (in analogy to the Friedrichs extension, which found natural applications in quantum mechanics, elasticity, etc.). In the second, and principal part of this survey, we study spectral properties for $H_{K,\Omega}$, the Krein--von Neumann extension of the perturbed Laplacian $-\Delta+V$ (in short, the perturbed Krein Laplacian) defined on $C^\infty_0(\Omega)$, where $V$ is measurable, bounded and nonnegative, in a bounded open set $\Omega\subset\mathbb{R}^n$ belonging to a class of nonsmooth domains which contains all convex domains, along with all domains of class $C^{1,r}$, $r>1/2$.Comment: 68 pages. arXiv admin note: extreme text overlap with arXiv:0907.144

Twenty five years after KLS: A celebration of non-equilibrium statistical mechanics

When Lenz proposed a simple model for phase transitions in magnetism, he couldn't have imagined that the "Ising model" was to become a jewel in field of equilibrium statistical mechanics. Its role spans the spectrum, from a good pedagogical example to a universality class in critical phenomena. A quarter century ago, Katz, Lebowitz and Spohn found a similar treasure. By introducing a seemingly trivial modification to the Ising lattice gas, they took it into the vast realms of non-equilibrium statistical mechanics. An abundant variety of unexpected behavior emerged and caught many of us by surprise. We present a brief review of some of the new insights garnered and some of the outstanding puzzles, as well as speculate on the model's role in the future of non-equilibrium statistical physics.Comment: 3 figures. Proceedings of 100th Statistical Mechanics Meeting, Rutgers, NJ (December, 2008