545 research outputs found

    Archaeologists as Artefacts. Towards a Biographical Understanding of Formation Processes in the History of Australian Archaeology

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    Archaeologists traditionally think of the past through things; through a base unit of analysis that is the artefact. In this thesis I argue that the basic unit in the study of the history of archaeology can also be archaeologists themselves, functioning as artefacts of the discipline. Further, that many of the analytical tools applied to the study of artefacts apply well metaphorically, to the study of the discipline as a whole. I argue for the need to evaluate the individual archaeological practitioner with reference to a collective assemblage - their community of practice, and also to observe and critique the interplay between individual practitioners. In this thesis I examine three liminal individuals in the history of Australian archaeology; Dermot Casey, Elsie Bramell and Alexander Gallus and I highlight the nature of their relationship to the discipline as a whole. The level of in-depth study I have applied to each of my case studies is necessary to tease out sufficient contextual understanding of individual circumstances. My findings from these three case studies is sufficient to robustly challenge the existing paradigm of the development and growth of Australian archaeology. To date, histories of Australian archaeology have portrayed the discipline as shifting abruptly from an amateur to professional state with the excavation of Fromms Landing by John Mulvaney in 1956. This chronology is grounded on Mulvaney’s own history of the discipline, and several subsequent works that largely accept Mulvaney’s proposition. My work indicates that this amateur-professional divide is untenable, as persons fitting either category of amateur or professional were active in Australian archaeology throughout the 20th century. I contend that the primary factors that have shaped Australian archaeology through the 20th century were biographical and social forces, enmeshed in and reifying archaeological disciplinary culture. While writing these biographies, I have been cognisant of the need for reflexivity. I have reflected on my own biographical influences in approaching my topic as much as I do in evaluating the effects of biographical factors on the archaeological careers of the three case study individuals. This is an intimately personal thesis – from my lived experience, to my analysis of other lives, for evaluation by you

    Rethinking Resident Perceptions of Tourism in British Columbia, Canada

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    This joint academic/practitioner report segments British Columbia, Canada residents to provide destination managers with new ways to better understand resident perceptions of tourism. The data collection was conducted in April and May of 2022 and had a total of 2,265 valid responses. It was also a practical objective to conduct this research in a manner that is repeatable in jurisdictions beyond British Columbia. This report has confirmed five distinct categories of residents’ perceptions toward tourism, including socio-cultural, economic, environmental, job/career, and Indigenous impacts. In addition to the categories of impacts, a cluster analysis has revealed six clusters of residents based on the five categories: Tourism Ambassadors, Tourism Supporters, Socio-cultural and Tourism Economic Supporters, Neutrals, Concerns about Careers and Environment, and Tourism Adversaries. Managerial implications and opportunities for future destination management and governance are discussed

    The synthesis of peptide-PNA conjugates

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    Abstract only availableIn two long term studies of non-Hodgkin's Lymphoma (NHL), the only clinical feature associated with a high relapse rate and treatment resistance is the presence of the B-cell lymphoma/leukemia-2(bcl-2) proto-oncogene in an over-active state. Regulation of this gene has shown promise as a means for better treatment in patients with relapsed NHL. At MU, in the past, many antisense and nonsense peptide-peptide nucleic acid (PNA) conjugates that target the bcl-2 proto-oncogene were synthesized and radiolabeled for mRNA binding evaluations. This summer, we synthesized two new peptide-peptide nucleic acid conjugates. The first of these was a nonsense sequence of PNA monomers attached to the peptide Tyrosine-3-Octreotate (1), and the second was the anti-bcl-2 sequence attached to Alanine-box Octreotate (2). The PNA sequences attached to the peptides correspond to the first fourteen bases of the bcl-2 proto-oncogene (ccagcgtgcgccat) in the case of the anti-bcl-2 compound (2) and correspond to no matching sequence in the human genome in the case of the nonsense sequence (1). They are both for later use as a negative control in mRNA binding evaluations (the previously synthesized positive agent being anti-bcl-2 attached to Tyrosine-3-Octreotate). The peptides were synthesized using standard Fmoc solid phase peptide synthesis on a resin using a very low substitution level. The peptides were synthesized in an automatic peptide synthesizer, and then elongated with the addition of the PNA monomers manually in a reaction vessel. Each peptide-PNA construct was then coupled to DOTA (1, 4, 7, 10-tetraazacyclododecane-N, N', N'', N'''-tetraacetic acid), a ligand to provide a site for subsequent radiometal chelation. The correct molecular weight with a high purity was observed in the LC-MS results for the peptide-PNA compound 1 after two attempts, and compound 2 was successful after the first attempt. The amount of each construct synthesized should be enough for the later mRNA binding study.Molecular Imaging Progra
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