In vivo analysis of NHPX reveals a novel nucleolar localization pathway involving a transient accumulation in splicing speckles

The NHPX protein is a nucleolar factor that binds directly to a conserved RNA target sequence found in nucleolar box C/D snoRNAs and in U4 snRNA. Using enhanced yellow fluorescent protein (EYFP)– and enhanced cyan fluorescent protein–NHPX fusions, we show here that NHPX is specifically accumulated in both nucleoli and Cajal bodies (CBs) in vivo. The fusion proteins display identical localization patterns and RNA binding specificities to the endogenous NHPX. Analysis of a HeLa cell line stably expressing EYFP–NHPX showed that the nucleolar accumulation of NHPX was preceded by its transient accumulation in splicing speckles. Only newly expressed NHPX accumulated in speckles, and the nucleolar pool of NHPX did not interchange with the pool in speckles, consistent with a unidirectional pathway. The transient accumulation of NHPX in speckles prior to nucleoli was observed in multiple cell lines, including primary cells that lack CBs. Inhibitor studies indicated that progression of newly expressed NHPX from speckles to nucleoli was dependent on RNA polymerase II transcription, but not on RNA polymerase I activity. The data show a specific temporal pathway involving the sequential and directed accumulation of NHPX in distinct subnuclear compartments, and define a novel mechanism for nucleolar localization

Determination of the internal structure of neutron stars from gravitational wave spectra

In this paper the internal structure of a neutron star is shown to be inferrable from its gravitational-wave spectrum. Iteratively applying the inverse scheme of the scaled coordinate logarithmic perturbation method for neutron stars proposed by Tsui and Leung [Astrophys. J. {\bf 631}, 495 (2005)], we are able to determine the mass, the radius and the mass distribution of a star from its quasi-normal mode frequencies of stellar pulsation. In addition, accurate equation of state of nuclear matter can be obtained from such inversion scheme. Explicit formulas for the case of axial $w$-mode oscillation are derived here and numerical results for neutron stars characterized by different equations of state are shown.Comment: 26 pages, 14 figures, submitted to Physical Review

Perturbative Analysis of Universality and Individuality in Gravitational Waves from Neutron Stars

The universality observed in gravitational wave spectra of non-rotating neutron stars is analyzed here. We show that the universality in the axial oscillation mode can be reproduced with a simple stellar model, namely the centrifugal barrier approximation (CBA), which captures the essence of the Tolman VII model of compact stars. Through the establishment of scaled co-ordinate logarithmic perturbation theory (SCLPT), we are able to explain and quantitatively predict such universal behavior. In addition, quasi-normal modes of individual neutron stars characterized by different equations of state can be obtained from those of CBA with SCLPT.Comment: 29 pages, 10 figures, submitted to Astrophysical Journa

Prelaunch absolute radiometric calibration of LANDSAT-4 protoflight Thematic Mapper

Results are summarized and analyzed from several prelaunch tests with a 122 cm integrating sphere used as part of the absolute radiometric calibration experiments for the protoflight TM sensor carried on the LANDSAT-4 satellite. The calibration procedure is presented and the radiometric sensitivity of the TM is assessed. The internal calibrator and dynamic range after calibration are considered. Tables show dynamic range after ground processing, spectral radiance to digital number and digital number to spectral radiance values for TM bands 1, 2, 3, 4, 5, 7 and for channel 4 of band 6

Prelaunch absolute radiometric calibration of the reflective bands on the LANDSAT-4 protoflight Thematic Mapper

The results of the absolute radiometric calibration of the LANDSAT 4 thematic mapper, as determined during pre-launch tests with a 122 cm integrating sphere, are presented. Detailed results for the best calibration of the protoflight TM are given, as well as summaries of other tests performed on the sensor. The dynamic range of the TM is within a few per cent of that required in all bands, except bands 1 and 3. Three detectors failed to pass the minimum SNR specified for their respective bands: band 5, channel 3 (dead), band 2, and channels 2 and 4 (noisy or slow response). Estimates of the absolute calibration accuracy for the TM show that the detectors are typically calibrated to 5% absolute error for the reflective bands; 10% full-scale accuracy was specified. Ten tests performed to transfer the detector absolute calibration to the internal calibrator show a 5% range at full scale in the transfer calibration; however, in two cases band 5 showed a 10% and a 7% difference

Radiometric calibration and processing procedure for reflective bands on LANDSAT-4 protoflight Thematic Mapper

The radiometric subsystem of NASA's LANDSAT-4 Thematic Mapper (TM) sensor is described. Special emphasis is placed on the internal calibrator (IC) pulse shapes and timing cycle. The procedures for the absolute radiometric calibration of the TM channels with a 122-centimeter integrating sphere and the transfer of radiometric calibration from the channels to the IC are reviewed. The use of the IC to calibrate TM data in the ground processing system consists of pulse integration, pulse averaging, IC state identification, linear regression analysis, and histogram equalization. An overview of the SCROUNGE-era (before August 1983) method is presented. Procedural differences between SCROUNGE and the TIPS-era (after July 1983) and the implications of these differences are discussed

Characterization of radiometric calibration of LANDSAT-4 TM reflective bands

Prelaunch and postlaunch internal calibrator, image, and background data is to characterize the radiometric performance of the LANDSAT-4 TM and to recommend improved procedures for radiometric calibration. All but two channels (band 2, channel 4; band 5, channel 3) behave normally. Gain changes relative to a postlaunch reference for channels within a band vary within 0.5 percent as a group. Instrument gain for channels in the cold focal plane oscillates. Noise in background and image data ranges from 0.5 to 1.7 counts. Average differences in forward and reverse image data indicate a need for separate calibration processing of forward and reverse scans. Precision is improved by increasing the pulse integration width from 31 to 41 minor frames, depending on the band