Velocity of Ultrasonic Waves in Solutions of Electrolytes

List of the genes involved in CVDs according to DisGeNET database. For each gene all the related diseases and the putative EV-MiRNAs targeting it are indicated both as list and as number of occurrences. (PDF 1206 kb

General descriptive statistics for paired maternal-cord blood samples included in the analysis.

<p>General descriptive statistics for paired maternal-cord blood samples included in the analysis.</p

Spearman correlation coefficients between umbilical cord blood and maternal blood for each epigenetic marker.

‡<p>0.05≥p≥0.01.</p>†<p>0.01>p>0.0001.</p

Examples of pyrograms.

<p>A) <i>LINE-1</i>, B) <i>Alu</i>, C) <i>p16</i>, and D) <i>p53</i>.</p

Primers used for DNA methylation analysis.

a<p>Nucleotides at which DNA methylation was measured are underlined.</p

Localization of gene promoters and regions amplified and of the CpG dinucleotide positions at which DNA methylation was quantified.

<p>Localization of gene promoters and regions amplified and of the CpG dinucleotide positions at which DNA methylation was quantified.</p

Blood DNA methylation levels (%mC) in Day 1 and Day 4 samples.

<p>Blood DNA methylation levels (%mC) in Day 1 and Day 4 samples.</p

Representative scatter plots (Day 1 vs. Day 4 blood DNA methylation measures) of the biomarkers with highest (<i>IL-6</i>, panel A) and lowest (<i>APC</i>, panel B) intra-class correlation coefficients.

<p>Representative scatter plots (Day 1 vs. Day 4 blood DNA methylation measures) of the biomarkers with highest (<i>IL-6</i>, panel A) and lowest (<i>APC</i>, panel B) intra-class correlation coefficients.</p

Correlations of intraclass correlation coefficients (ICCs) with DNA methylation levels and genomic characteristics of the sequences analyzed.

<p>The panels show correlations of ICCs for each of the methylation biomarkers with content of guanosine and cytosine (G+C, panel A); ratio of observed/expected CpG dinucleotides (CpG o/e; panel B); distance of repeat elements from 3′ (panel C); distance of repeat elements from 5′ (panel D); DNA methylation mean on Day 1 (panel E); range of DNA methylation on Day 1 (panel F). The scatter plots use ICC values subtracted of pyrosequencing measurement errors (ICC₁) and estimated from models adjusted by PM₁₀ exposure levels, age, current smoking, and percent blood granulocytes. Each data point corresponds to the ICC₁ value for one biomarker, as indicated by the corresponding label.</p

Sequence characteristics of the DNA methylation markers analyzed.

*<p><i>Alu</i> and LINE-1 were not considered, as repeat elements have multiple locations across the human genome with different context sequence characteristics.</p