Additional file 1: Figure S1. of Identification of repressive and active epigenetic marks and nuclear bodies in Entamoeba histolytica

The N-terminal region of histone H4 of E. histolytica is conserved among different eukaryotic cells. A) Alignment of the N-terminal region of histone H4 from E. histolytica (Eh), P. falciparum (Pf), Saccharomyces cerevisiae (Sc), Drosophila melanogaster (Dm), and Homo sapiens (Hs). (*). Identical residues; (:) compensatory changes. Three insertions present in the N-terminal region of histone H4 of E. histolytica are indicated in red letters. B) Elimination of the three insertions present in the N-terminal region of histone H4 of Eh (indicated by lines) generates a highly conserved N-terminal region among Eh, Pf, Sc, Dm and Hs. Alignment of the H4 N-terminal region of Eh, Pf, Sc, Dm and Hs with H4 pan-acetylated peptide from Tetrahymena thermophiles and a H4 arginine 3 mono-methylated peptide are shown. Rectangle indicated the most conserved residues identified for both peptides. Lysines and arginine amino acids susceptible to be acetylated are indicated in bold. (DOCX 27 kb