Plains zebra (Equus quagga) behaviour in a restored population reveals seasonal resource limitations

A once abundant species, plains zebra (Equus quagga), is declining across much of sub-Saharan Africa. Reintroduction efforts at Majete Wildlife Reserve, Malawi, have resulted in rapid population increases, but little is known about how such populations resemble natural populations socially or behaviourally, and what those attributes may reveal about restoration success. Incorporating behavioural knowledge into conservation efforts is an important tool for managing the effects of habitat fragmentation and resource competition. The aim of this study was to quantify the daylight time budget of both family and bachelor bands of reintroduced plains zebra to determine if such behaviours resembled those found in natural populations, and to provide insights into seasonal behavioural patterns that could inform management strategies. We found that feeding occupied the largest percentage (mean = 41.8 % ± 2.36) of family band daylight time budgets, followed by resting (18.5 % ± 2.21), locomotion (10.9 % ± 1.05), vigilance (7.5 % ± 0.92), maintenance (2.7 % ± 0.92) and social behaviour (1.4 % ± 0.33). Bachelor bands spent the majority of their daylight time being vigilant (27.0 % ± 2.72), followed by locomotion (21.0 % ± 2.05), feeding (18.4 % ± 2.32), resting (15.4 % ± 2.85), maintenance (6.4 % ± 1.86) and social behaviour (2.4 % ± 0.68). The time budgets of zebra in this restored population are generally congruent with those reported in natural populations of plains zebra. Seasonal variation in time spent feeding and resting, locomotion, and change in body condition, however, suggest that dry season resources may negatively impact zebra in the miombo savannah woodlands. These results provide important insights into resource implications for zebra in an ecological system with many large ungulate species that compete for forage, and may assist managers with resource management strategies

High resolution mapping of Twist to DNA in Drosophila embryos: Efficient functional analysis and evolutionary conservation

Cis-regulatory modules (CRMs) function by binding sequence specific transcription factors, but the relationship between in vivo physical binding and the regulatory capacity of factor-bound DNA elements remains uncertain. We investigate this relationship for the well-studied Twist factor in Drosophila melanogaster embryos by analyzing genome-wide factor occupancy and testing the functional significance of Twist occupied regions and motifs within regions. Twist ChIP-seq data efficiently identified previously studied Twist-dependent CRMs and robustly predicted new CRM activity in transgenesis, with newly identified Twist-occupied regions supporting diverse spatiotemporal patterns (>74% positive, n = 31). Some, but not all, candidate CRMs require Twist for proper expression in the embryo. The Twist motifs most favored in genome ChIP data (in vivo) differed from those most favored by Systematic Evolution of Ligands by EXponential enrichment (SELEX) (in vitro). Furthermore, the majority of ChIP-seq signals could be parsimoniously explained by a CABVTG motif located within 50 bp of the ChIP summit and, of these, CACATG was most prevalent. Mutagenesis experiments demonstrated that different Twist E-box motif types are not fully interchangeable, suggesting that the ChIP-derived consensus (CABVTG) includes sites having distinct regulatory outputs. Further analysis of position, frequency of occurrence, and sequence conservation revealed significant enrichment and conservation of CABVTG E-box motifs near Twist ChIP-seq signal summits, preferential conservation of ±150 bp surrounding Twist occupied summits, and enrichment of GA- and CA-repeat sequences near Twist occupied summits. Our results show that high resolution in vivo occupancy data can be used to drive efficient discovery and dissection of global and local cis-regulatory logic

The VLA/ALMA Nascent Disk and Multiplicity (VANDAM) Survey of Perseus Protostars. VI. Characterizing the Formation Mechanism for Close Multiple Systems

We present Atacama Large Millimeter/submillimeter Array (ALMA) observations of multiple protostar systems in the Perseus molecular cloud previously detected by the Karl G. Jansky Very Large Array (VLA). We observed 17 close ($<$600~AU separation) multiple systems at 1.3~mm in continuum and five molecular lines (i.e., \twco, \cateo, \thco, H$_2$CO, SO) to characterize the circum-multiple environments in which these systems are forming. We detect at least one component in the continuum for the 17 multiple systems. In three systems, one companion is not detected, and for two systems the companions are unresolved at our observed resolution. We also detect circum-multiple dust emission toward 8 out of 9 Class 0 multiples. Circum-multiple dust emission is not detected toward any of the 8 Class I multiples. Twelve systems are detected in the dense gas tracers toward their disks/inner envelopes. For these 12 systems, we use the dense gas observations to characterize their formation mechanism. The velocity gradients in the circum-multiple gas are clearly orthogonal to the outflow directions in 8 out of the 12 systems, consistent with disk fragmentation. Moreover, only two systems with separations $<$200~AU are \textit{inconsistent} with disk fragmentation, in addition to the two widest systems ($>$500~AU). Our results suggest that disk fragmentation via gravitational instability is an important formation mechanism for close multiple systems, but further statistics are needed to better determine the relative fraction formed via this method.Comment: 48 Pages, 26 Figures, 7 Tables, Accepted by Ap

Applying psychological type theory to cathedral visitors : a case study of two cathedrals in England and Wales

This study employs Jungian psychological type theory to profile visitors to Chester Cathedral in England and St Davids Cathedral in Wales. Psychological type theory offers a fourfold psychographic segmentation of visitors, distinguishing between introversion and extraversion, sensing and intuition, thinking and feeling, and judging and perceiving. New data provided by 157 visitors to Chester Cathedral (considered alongside previously published data provided by 381 visitors to St Davids Cathedral) demonstrated that these two cathedrals attract more introverts than extraverts, more sensers than intuitives, and more judgers than perceivers, but equal proportions of thinkers and feelers. Comparison with the population norms demonstrated that extraverts and perceivers are significantly under-represented among visitors to these two cathedrals. The implications of these findings are discussed both for maximising the visitor experiences of those already attracted to these cathedrals and for discovering ways of attracting more extraverts and more perceivers to explore these cathedrals

Biology and therapy of fibromyalgia. Evidence-based biomarkers for fibromyalgia syndrome

Researchers studying fibromyalgia strive to identify objective, measurable biomarkers that may identify susceptible individuals, may facilitate diagnosis, or that parallel activity of the disease. Candidate objective measures range from sophisticated functional neuroimaging to office-ready measures of the pressure pain threshold. A systematic literature review was completed to assess highly investigated, objective measures used in fibromyalgia studies. To date, only experimental pain testing has been shown to coincide with improvements in clinical status in a longitudinal study. Concerted efforts to systematically evaluate additional objective measures in research trials will be vital for ongoing progress in outcome research and translation into clinical practice

The SAMI Galaxy Survey: Unveiling the nature of kinematically offset active galactic nuclei

We have observed two kinematically offset active galactic nuclei (AGN), whose ionised gas is at a different line-of-sight velocity to their host galaxies, with the SAMI integral field spectrograph (IFS). One of the galaxies shows gas kinematics very different to the stellar kinematics, indicating a recent merger or accretion event. We demonstrate that the star formation associated with this event was triggered within the last 100 Myr. The other galaxy shows simple disc rotation in both gas and stellar kinematics, aligned with each other, but in the central region has signatures of an outflow driven by the AGN. Other than the outflow, neither galaxy shows any discontinuity in the ionised gas kinematics at the galaxy's centre. We conclude that in these two cases there is no direct evidence of the AGN being in a supermassive black hole binary system. Our study demonstrates that selecting kinematically offset AGN from single-fibre spectroscopy provides, by definition, samples of kinematically peculiar objects, but IFS or other data are required to determine their true nature.Comment: MNRAS accepted. 14 pages, 11 figure

The Trans-Pacific Partnership Agreement: Looking Ahead to the Next Steps

Pressure has been building for the conclusion of the 12-country Trans-Pacific Partnership (TPP) negotiations. Getting the deal done is important, but the TPP is not just another free trade agreement (FTA). It represents the chance to set a trade agenda for the future across a wide range of topics for countries throughout the Asia-Pacific region. This means that the agreement should not be settled in haste. More importantly, it also means that key decisions need to be reached about broader issues related to the institutional structure of the TPP. These decisions must be made now, before the deal is closed, on issues such as how to create the TPP as a living agreement, the formation of a TPP Secretariat, and the clarification of entry conditions for future members such as the People’s Republic of China (PRC). These choices must be made deliberately and carefully even while officials are struggling with reaching closure on the most highly sensitive issues still remaining in the agreement. It will not be easy, but wise decisions are necessary now to ensure the long-term success of the TPP