Effect of the improved protein-mineral additive on structural-mechanical characteristics of minced meat

The results of research on the effect of protein-mineral improved additive (PMIA) on the rheological parameters of minced meat, which can be used for manufacturing culinary products, including chopped semi-finished products of a high degree of readiness, are presented. It has been proven, that the additive can be used both as an ingredient, enriching calcium-digestible compounds and to improve a number of technological properties of minced meat, in particular after freezing, storage and thawing. The aim of the study was to determine the dependences of changes in the structural and mechanical characteristics of minced meat after the addition of PMIA and subsequent freezing to a temperature of –16…–18 °C and storage for 20 days. It has been found, that the addition of up to 7 % of PMIA leads to a marked increase in the conditionally instantaneous modulus of elasticity and highly elastic modulus in 4.4 times for minced beef and 2.7 and 4.4 times for chicken, respectively. It has been established, that the best stabilization of these indicators after freezing occurs at the content of PMIA at the level of 2…5 %. Studies of plastic viscosity and adhesion have shown that the use of up to 7 % of PMIA leads to an increase of 11…20 % and 26…64 %, respectively. After freezing, the plastic viscosity and adhesion of minced beef in the control decreased by 22.0 and 52 %, respectively, minced chicken – by 23.4 and 40.9 %. In the samples with a content of 7 % of PMIA, the decrease in plastic viscosity and adhesion is 7.2 and 4.4 % in minced beef and in chicken – 5.9 and 3.1 % respectively. It has been proven, that the use of PMIA in the amount of up to 7 % in the technology of minced meat production minimizes the negative destructive effect of low temperatures on the structural and mechanical characteristics of the finished product. Thus, it is expedient to use up to 7.0 % of the improved protein-mineral additive in the composition of minced meat to enrich the finished product with digestible calcium compounds and improve their structural and mechanical characteristics, in particular after freezing and storag

Carbenoxolone does not cross the blood brain barrier: an HPLC study

BACKGROUND: Carbenoxolone (CBX) is a widely used gap junctional blocker. Considering several reports indicating that transient gap junctional blockade could be a favourable intervention following injuries to central nervous tissue, and some current enthusiasm in studies using systemic injections of CBX, it is imperative to consider the penetration of CBX into central nervous tissue after systemic administrations. So far, only very indirect evidence suggests that CBX penetrates into the central nervous system after systemic administrations. We thus determined the amounts of CBX present in the blood and the cerebrospinal fluid of rats after intraperitoneal administration, using high performance liquid chromatography RESULTS: CBX was found in the blood of the animals, up to 90 minutes post-injection. However, the cerebrospinal fluid concentration of CBX was negligible. CONCLUSION: Thus, we conclude that, most likely, CBX does not penetrate the blood brain barrier and therefore recommend careful consideration in the manner of administration, when a central effect is desired

Carbenoxolone does not cross the blood brain barrier: an HPLC study

Abstract Background Carbenoxolone (CBX) is a widely used gap junctional blocker. Considering several reports indicating that transient gap junctional blockade could be a favourable intervention following injuries to central nervous tissue, and some current enthusiasm in studies using systemic injections of CBX, it is imperative to consider the penetration of CBX into central nervous tissue after systemic administrations. So far, only very indirect evidence suggests that CBX penetrates into the central nervous system after systemic administrations. We thus determined the amounts of CBX present in the blood and the cerebrospinal fluid of rats after intraperitoneal administration, using high performance liquid chromatography Results CBX was found in the blood of the animals, up to 90 minutes post-injection. However, the cerebrospinal fluid concentration of CBX was negligible. Conclusion Thus, we conclude that, most likely, CBX does not penetrate the blood brain barrier and therefore recommend careful consideration in the manner of administration, when a central effect is desired