5 research outputs found
Fully formed contact plate immunogold stained.
<p>Staining conditions are the same as on <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046542#pone-0046542-g005" target="_blank">Fig. 5</a>. a βcontact plate of the oocyte VII final stage at low magnification. Indicated with lines at the right are: O β oocyte, CP β contact plate, ge β germinal epithelium. Scale bar β5 Β΅m. aβ² β high magnification of the part of the image on a; gold particles pseudocolored yellow. Scale barβ2 Β΅m. b β type 2 granule presumably in the process of excretion (not stained). Scale bar β 2 Β΅m.</p
Ultrastructure of medusas' growing oocyte.
<p>I, III, IV, VI, VII β stages of medusas' oocyte development; the same structures as on <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046542#pone-0046542-g002" target="_blank">fig. 2</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046542#pone-0046542-g003" target="_blank">fig. 3</a> are indicated and in addition: mt β mitochondrion, Y β yolk granule. Granules are indicated with black arrows and granule type is indicated with 1 and 2 on b (III), c (IV) and d (VI). Germinal epithelium protrusions are indicated by black arrows with an asterisk on c (IV) and e (VI). bβ² and bβ³ represent the high magnification of parts from b (III). Scale bar β 5 Β΅m for a (I), b (III), c (IV), d (VI), e (VI). Scale Bar β 2 Β΅m for bβ² (type 2 granule) and bβ³ (type 1 granule).</p
Fertilization <i>in vitro</i>.
<p>Spermatozoids added to medusa eggs <i>in vitro</i> in sea water. a - phase contrast microscope, squash preparation. N β eggs' nucleus, sp β spermatozoids. Scale bar β 10 Β΅m. b β cβ² β whole egg in the process of fertilization (non-squashed); b β DAPI staining in grayscale, low magnification; c and cβ² - phase contrast and DAPI fluorescence in grayscale, the same field. Scale bar β 50 Β΅m.</p
Electrophoresis and immunoblot of medusa cell types.
<p>I β SDS-PAGE (a) and immunoblot (b). a β Coomassie-stained 10% SDS-PAGE: 1 (Mes) β mesoglea of adult <i>A.aurita</i>; 2 (Ect) β ectodermal tissue layer; 3 (Mc) β mesogleal cells; 4 (Gf) β germinal epithelium of female gonad. <b><i>M<sub>r</sub></i></b> of marker proteins in kDa are indicated on the left. b β immunoblot of the correspondent lines - Mes, Ect, Mc and Gf. II β AU-PAGE (a) and immunoblot (b). a β Coomassie-stained 7% AU-PAGE: 0 β hialuronidase (pI 9.2) marked with asterisk; 1 (Mes) β mesoglea of adult <i>A.aurita</i>; 2 (Ect) β ectodermal tissue layer; 3 (Mc) β mesogleal cells; 4 (Gf) β germinal epithelium of females' gonad. Arrow marks the start of the separating gel. b β immunoblot. For both immunoblots (b) - primary antibodies RA47 in final dilution 1βΆ5000; secondary antibodies were antirabbit IgG conjugated with alkaline phosphatase in final dilution 1βΆ20000 (Sigma).</p
Immunostaining on paraffin sections of medusa female s' gonads.
<p>I, IV, VI, VII β development stages of medusa oocyte. First row - phase contrast; second row β immunostaning of the same preparation. Indicated: Mes β adjacent mesoglea, O β oocyte, N β oocytes' nuclei, g β granules in the oocyte; CP β contact plate. For immunostaining RA47 in final dilution 1βΆ2000; antirabbit AB conjugated with rhodamine in final dilution 1βΆ200 (Sigma). Scale bar β 10 Β΅m.</p