(A) 2-DE map of OT obtained by performing IEF on a 4–7 linear pH range and SDS-PAGE on a constant 12,5% T in the second dimension, to separate proteins clustered in the single spot shown in Fig 4. (B) Immunoblotting of the gel slab indicated in Panel A.

<p>Arrow points to spot originated from separation and identified as FliD.</p

List of proteins identified under the immunoreactive spot.

<p>List of proteins identified under the immunoreactive spot.</p

Set of panels showing the density variances between SG and OT pools for spots 1 to 10.

<p>In each panel the region of the stained gel containing the spot of interest was magnified (inset) and the up-/downregulation graphically represented. Pvalue indicating statistical significant density variance (T-test) is reported in each panel.</p

Immunoblotting of proteins from SG and OT profiles generated as indicated in Fig 1.

<p>PVDF membranes were incubated with the rabbit polyclonal antibodies anti-FliD of <i>M</i>. <i>mitochondrii</i>, followed by anti-rabbit antibody. The protein spot(s) indicated by an arrow in Panel A (OT pool) was tentatively assigned to FliD. Panel B shows the SG profile in which the hypothetical FliD spot is undetectable.</p

High Master gel, showing qualitative differences between the SG and OT 2-DE master gel patterns (NL, pH 3–10 gradient range).

<p>Labelled in green: spots (n = 170 ± 25) common to both SG and OT. Labelled in red: spots (n = 81) exclusive of SG. Labelled in blue: spots (n = 57) detected solely in OT.</p