ΔNRG4 mRNA expression for each patient.

<p>Waterfall plot depicting the difference in NRG4 mRNA expression between the tumor tissue and the normal tissue for each patient (ΔNRG4). The difference is calculated as the tumor tissue expression minus the normal tissue expression, and hence, a negative value represents a downregulation in the tumor tissue compared to the normal tissue. The tumor localization of the individual patients is depicted in the figure.</p

Primers and annealing temperature for the qPCR reactions used in the study.

<p>Primers and annealing temperature for the qPCR reactions used in the study.</p

Clinical characteristics of the patients included in this study.

<p>Clinical characteristics of the patients included in this study.</p

Immunohistochemical staining for HER4 protein expression in normal and cancer tissue.

<p><b>A</b>) Normal gastric mucosa with immunoreaction to HER4 primarily in the surface epithelium but also in the glands. <b>B</b>) larger magnification of the gastric epithelium. The immunoreaction is localized basolaterally in the surface epithelial cells – both as a cytoplasmatic and membrane staining. <b>C</b>) In the glands the most intense staining was seen in the parietal cells. These (<b>D</b>)) were easily identified by the presence of intracellular cannaliculi. <b>E</b>) The normal mucosa with strong immunoreactions in the upper part of the figure contrasts with underlying negative tumor tissue. Magnifications <b>A</b>×40, <b>B</b>,<b>G</b>×210, <b>C</b>×130, <b>D</b>×600; <b>E</b>×20.</p

ΔHER4 mRNA expression for each patient.

<p>Waterfall plot depicting the difference in total HER4 mRNA expression between the tumor tissue and the normal tissue for each patient (ΔHER4). The difference is calculated as the tumor tissue expression minus the normal tissue expression, and hence, a negative value represents a downregulation in the tumor tissue compared to the normal tissue. The tumor localization of the individual patients is depicted in the figure.</p

mRNA expressions of the alternatively spliced isoforms of HER4.

<p>All mRNA expression concentrations are arbitrary and represent the ratio of mRNA expression of the target gene divided by the mRNA expression of the reference gene SDHA. mRNA expression was quantified in tumor biopsies from gastric cancer patients and the paired adjacent normal tissue from the same patient. P values are calculated using a paired t-test.</p

mRNA expressions of the four receptors of the EGF system.

<p>All mRNA expression concentrations are arbitrary and represent the ratio of mRNA expression of the target gene divided by the mRNA expression of the reference gene SDHA. mRNA expression was quantified in tumor biopsies from gastric cancer patients and the paired adjacent normal tissue from the same patient. P values are calculated using a paired t-test.</p

Quantification of HER4 immunostaining intensity in tumor tissue and normal tissue.

<p>The staining intensity of representative images of the tumor versus the surrounding mucosa tissue was estimated. Two images of tumor sample and two images of the normal mucosa were collected from each tissue sample and the average of the two values was calculated. P value was calculated using a paired t-test.</p

miR-21 expression is found in the tumor, endothelium and acinar cells in a subset of OSCCs.

<p>(A) An overview image showing the distribution of the miR-21 expression, which is highest in the central portion of the tumor (B) and is lower at the invasive tumor front (C). We found a strong miR-21 expression in the tumor cells from one specimen (D), which was also present in the budding tumor cells (arrow). miR-21 expression was also observed in the endothelial cells (E, arrow) and in the degenerated acinar cells (F, arrow).</p

Multivariate model of factors associated with disease free survival.

<p>Legend: The remaining factors in multivariate Cox regression after backwards elimination. Only miR-21 TBS and perineural invasion remained as independent factors influencing survival.</p