In Vitro and In Vivo Feedstuff Digestibility for Snook, Centropomus undecimalis , Juveniles

Abstract Thedegreeofhydrolysis(DH)oftheingredientswasevaluatedforCentropomusundecimalisjuveniles. The in vitro experiment included wheat gluten (WG), corn gluten (CG), soybean meal (SBM) and pretreatedphytase(SBM+phytase),soyproteinconcentrate(SPC),canolameal(CAN)andpretreated phytase (CAN+phytase), poultry byproduct meal (PBPM), chicken meal (CHKM), dried whey (DW), Pota meal (PM: mix of giant squid, fish soluble protein concentrate, crustacean meal, and fish oil), and Protiblend (PTB: aquatic and render mix). The highest acidic DH occurred with PTB (0.38±0.06), CHKM (0.33±0.3), and PBPM (0.25±0.03). In the alkaline condition, PTB (1.6±0.17 and 0.98±0.05 for pyloric caeca and intestine, respectively) and CG (1.04±0.4 and 0.75±0.2 for caeca and intestine, respectively) provided the highest DH values. In vitro digestibility demonstrated that PTB was the highest(85.3%)followedbyPBPM(51.4%),CAN+phytase(47.6%),CG(45.1%),andCHKM(46.5%). The in vivo experiment concerned the WG, CHKM, PBPM, PM, and PTB diets plus a reference diet with fishmeal (Ref diet). The total %DH was different (P0.05). The apparent digestive coefficients ranged from 89.8 to 92.9% for protein and from 68 to 71.4% for energy. KEYWORDS amino acids, in vitro and in vivo digestibility, pH-stat, protein, snoo