Effect of Ethanol Extract and Ethyl Acetate Fraction of Betel Nut (Areca catechu L.) in Colonic Goblet Cells of Mice (Mus musculus) Given Orally Infective Egg of Trichuris muris

BACKGROUND: Trichuris trichiura is a soil-transmitted parasitic worm commonly found in humid, tropical to subtropical areas, as well as poor sanitation area. These worms are cosmopolitan, especially in tropical and humid countries like Indonesia. This worm infection is more common in developing countries and more in children than adults due to poorer child self-hygiene. Worm disease is one of the common society diseases with 60% of children in Indonesia which are estimated to be affected. One species of T. trichiura worm that is often used in research on experimental animals is Trichuris muris. The administration of T. muris infective eggs can significantly increase the number of goblet cells in intestinal crypts of colon and cecum. Goblet cell hyperplasia will occur after exposure to high-dose T. muris (acute/200 infective eggs) in resistant mice. Various studies on the development of natural and traditional materials have been developing in the past few years to see the effects of betel nut on the number of goblet cells in the large intestine. AIM: The aim of this research is to determine the effect of ethanol extract and the ethyl acetate fraction of betel nut on the change in the number of goblet cells in male mice given orally T. muris infective eggs. METHODS: This study was an experimental study with a post-test only control group design in male mice (Mus musculus) which were given 200 infective eggs of T. muris. The study sample consisted of 70 mice divided into seven groups. Doses of the ethanol extract of betel nut are 100 mg/kg b.w. and 150 mg/kg b.w., and doses of ethyl acetate fraction are 100 mg/kg b.w. and 150 mg/kg b.w. The independent variable was ethanol extract and ethyl acetate fraction of betel nut. The dependent variable is the number of goblet cells. Mean differences in groups were tested by Mann–Whitney. RESULTS: Statistical analysis showed a significant difference of p < 0.05 in the colonic crypts and cecum. In Group K (+), there was no increase in the number of goblet cells (54.2000 ± 30.54864) compared to Group K (−) (79.0750 ± 11.79221) in the colon. There was an increase in goblet cells in the 150 mg/kg b.w. of ethanol extract group (64.1000 ± 29.50381), the 150 mg/kg b.w. of ethyl acetate fraction group (56.2250 ± 22.11035), and the 1 mg/20 g of albendazole group (68.3750 ± 11.30956) in the colon. Likewise, there was an increase in the number of goblet cells in the cecum. It can be concluded that 150 mg/kg b.w. of the ethanol extract of betel nut can effectively increase the number of goblet cells in the colon and cecum. CONCLUSION: Ethanol extract of betel nut 150 mg / kg of weight can effectively increase the number of goblet cells in the colon and caecum

The Effects of Calcium Supplementation on Blood Lead Levels and Short-term Memory of Chronically Exposed Children: A Clinical Trial Study

AIM: The purpose of this study is to determine the effects of calcium supplementation to decrease blood lead levels (BLLs) of children at high risk for chronic lead poisoning and to determine its effects on short-term memory. MATERIALS AND METHODS: Children aged 8–12 years lived in the highest traffic density in Medan randomly included in this quasi-experimental study, divided into two groups (control and supplementation group received tablet contain four hundred milligrams oral calcium twice daily for 3 months). Samples for BLLs were collected before and after 3 months of supplementation, and short-term memory measurements are carried out by picture and forward digital span test. Descriptive statistics were calculated at baseline and 3 months; comparison between before and after treatment was assessed with t-tests, p < 0.05 considered statistically significant. RESULTS: BLLs samples, who are exposed to lead for >6 months were ranging between 0.4–12 μg/dL. Median BLLs in supplementation group before treatment was 2.1 μg/dL and after treatment was 0.01 μg/dL (p < 0.01); difference between median in BLLs after treatment in supplementation group was 2.090 μg/dL (p = 0.004). Score memory picture in the supplementation group before treatment was 61.4 ± 24.83 and after treatment was 76.21 ± 15.97 (p<0.01). Score memory digital span in the supplementation group before treatment was 5 (3–7) and after treatment was 7 (5–7) (p < 0.01). CONCLUSION: Three months of oral calcium supplementation 400 mg twice daily for high-risk chronic lead poisoning children reduced BLLs significantly and improved their short-term memory

CURCUMIN AND ITS EFFECT ON PREECLAMPSIA: AS ANTI-INFLAMMATORY, ANALGESIC, AND ANTICOAGULANT

Objective: Based on the Indonesia Health Profile In 2014, almost 30% of maternal deaths at Indonesia in 2010 were caused by hypertension in pregnancy, which one of them was preeclampsia. Many theories said to be the risk factor, such as acute body inflammation. Its clinical symptoms are hypertension, upper abdominal pain, and haemorrhage. Nowadays, many studies have been developed to predict the onset of preeclampsia, one of which is COX-2. This study was the first one to examine the benefits of curcumin in preeclamptic patients around the world. This study aims to discover whether curcumin affects the level of COX-2, VAS, and anticoagulant factors in preeclamptic patients. Methods: This is true experimental research with a double-blind randomized design. 50 samples were collected within a period of 7 mo and were divided into 2 groups: Case and Control Group. A total of 3 blood tests were conducted: prior, 90 min, and 12 h after case/treatment. Results: 4 samples were eliminated during preparation. The total number of samples was 46; 23 in Control Group and 23 in Case Group. In this study, we found clinical changes in the level of COX-2, VAS and anticoagulant factors in patients given 100 mg of curcumin compared to those who were not. In addition, we also found a correlation between clotting time and bleeding time at T2 where both time factors became shorter. Conclusion: There was a statistically significant decrease in COX-2 level in the Case Group, which led to decreased value of VAS, shorter clotting time and lower thrombocyte count; and, there was a significant correlation found between clotting time and bleeding time at T2 in the preeclamptic patients

Curcumin’s Effect оn COX-2 аnd IL-10 Serum in Preeclampsia’s Patient Undergo Sectio Caesarea with Spinal Anesthesia

BACKGROUND: Curcumin is a major component in curcuminoid which acts as an anti-inflammatory agent. Curcumin affects several biological markers that are thought to play a role in the pathogenesis of preeclampsia such as IL-10 and COX-2, resulting in an improvement in pregnant women with preeclampsia. AIM: To see the effect of perioperative curcumin administration on IL-10 and COX-2 in preeclamptic patients undergoing caesarean section under spinal anaesthesia. METHODS: This study was a double-blind, randomised clinical trial conducted at Pirngadi Hospital, USU Hospital and Sundari General Hospital Medan as a hospital network of Faculty of Medicine, North Sumatra University. Group 1 received a drug containing curcumin (as a treatment), and another group received a placebo (as a control). RESULTS: There were no significant differences in the median values of COX-2 and IL-10 before and after treatment and also the p-values were greater than 0.05 in both groups (control and treatment). CONCLUSION: There is no significant difference between the use of curcumin on serum COX-2 and IL-10 levels

THE EFFECT OF GAMBIER EXTRACT ON THE LEVELS OF MALONDIALDEHYDE, SUPEROXIDE DISMUTASE, AND BLOOD GLUCOSE IN TYPE 2 DIABETES MELLITUS PATIENTS

Objective: Diabetes mellitus is a disease associated with dysfunction of pancreatic β-cells and oxidative stress. A treatment which can reduce the impact of oxidative stress may be beneficial in the treatment of diabetes. Therefore, this study aimed to investigate the effect of gambier extract on the levels of malondialdehyde (MDA), superoxide dismutase (SOD), and blood glucose level (BGL) in type 2 diabetes mellitus (T2DM).Methods: This research was a randomized clinical study consisted of two groups, namely placebo group (n=10) and gambier group (n=6). The blood samples were collected from the vein after fasting overnight and before consuming 100 g white bread to measure the levels of MDA, SOD, and BGL. The same procedure was conducted after fasting and 2-h postprandial on day 1 and day 14. The data obtained were analyzed with Student's t-test with a statistical significance level of p<0.05.Results: The results showed that there was no change in MDA levels in the placebo group during the observation, but there was a significant decrease in MDA levels in the gambier group on day 14. In contrast, SOD levels increased in all measurements although there was no change on day 1 in the placebo group. The present study also found a significant increase of BGLs after consuming 100 g bread in both groups, but less BGL elevation in the gambier group.Conclusion: It is concluded that gambier extract has special mechanisms in the treatment of T2DM as an antioxidant and BGLs reduction

The Effect of Torbangun Leaves Tea on Msg-induced Fetal Develop-ment Disorder in Mice

The safety of MSG on the fetus has not been confirmed yet. The objective of this research is to determine the effect of tea of torbangun leaves on fetal development disorder of mice. The present study is experimental with a Completely Randomized Design (CRD). Data obtained were then analyzed by ANOVA, with p<0,05 as the level of significant.   The comparison of mean values ± SEM increase number of live fetuses  group C1-T1 (4,60 ± 0,93; 11,40 ± 0,87), p=0,018. The comparison of mean values ± SEM decrease number of embryo re-sorption percentage group C0-C1 (00,00 ± 00,00; 36,74 ± 15,13), group C1-C2 (36,74 ± 15,13; 00,00 ± 0,00), group C1-T1 (36,74 ± 15,13; 00,00 ± 0,00), and group C1-T2 (36,74 ± 15,13; 15,00 ± 22,36), p=0,020. This study concluded that T1 group is giving the best effect on increasing life fetuses and decreasing embryo re-sorption percentage

Increasing Atherosclerosis in Streptozotocin-Induced Diabetes into Four Groups of Mice

AIM: To study the protective effect of medicines on the formation of atherosclerosis in mice, it is needed to conduct the study in mice which is not genetically diabetic mice induced by streptozotocin (STZ) to produce hyperglycemia and atherosclerosis, compared with mice treated by yolk or its combination.MATERIAL AND METHODS: Fifty-six mice, Double Deutch Webster strain, male, receive  10 weeks, 20 - 30 gr bodyweight were divided into 4 groups (n = 14) i.e. control (do not received any agents), STZ (45 mg/kg/BW was injected intraperitoneally for 5 days), yolk (0.2 cc orally daily for 6 weeks), and combination of STZ and yolk (STZ: 45 mg/kg/BW intraperitoneally add 0.2 cc yolk orally). All animals were executed in the 42nd day. Then, the aorta of the mice’s heart tissue was histopathology examined. Blood glucose and cholesterol levels were determined every week.RESULTS: Hyperglycemia occurred in mice induced by STZ injection with the highest BGL (521.8 ± 48.2 mg/dl; 188.4%) in the 4th-week observation; after that BGL decrease. We found that, except the control, all treatment groups with STZ, egg yolk, and combination underwent atherosclerosis.CONCLUSION: The present study was able to demonstrate the occurrence of atherosclerosis in mice treated by STZ accompanied with increasing blood glucose and cholesterol level

COMBINATION OF WHOLE BRAIN RADIOTHERAPY WITH DIFFERENT FRACTION AND CONCOMITANT CAPECITABINE IN BRAIN METASTASIS BREAST CANCER

Objective: Breast cancer is the second most frequent cancer worldwide. The main therapeutic modality for breast cancer with brain metastasis is radiation. Whole Brain Radiotherapy (WBRT) is a regional treatment that provides moderate doses of radiotherapy to all brain tissue. Capecitabine was found to be effective for the treatment of breast cancer with metastasis. This study aims to determine the effectiveness of WBRT on the response of breast cancer brain metastatic lesions combined with capecitabine administration. Methods: This study uses a prospective, randomized-blind cohort analytic study approach. Subjects were randomized into two groups by giving different fraction of WBRT and capecitabine. Subjects were evaluated 4 w post-radiation. Data on differences in patient responses in the two treatment groups were analyzed. Results: A total of 23 breast cancer patients with brain metastasis participated in this study. Group I (WBRT 10x3Gy + capecitabine 1000 mg/m2/b.i.d) obtained results of 5 (45.5%) out of 11 are responding to therapy. Whereas in group II (WBRT 20x2Gy + capecitabine1000 mg/m2/b.i.d) found 11 (91.7%) out of 12 patients responded to therapy. The results of statistical analysis showed that there were significant differences between the two groups with a value of P = 0.027. Conclusion: Giving capecitabine and WBRT with 20x2Gy gives a better response both clinically and statisticall