Association between REG4 expression and clinicopathologic parameters in non-mucinous colorectal cancer.

<p>Exact Pearson chi-square test for 2×2 tables and exact linear-by-linear association test for tables with ordered variables. Missing data excluded from analyses.</p><p>Association between REG4 expression and clinicopathologic parameters in non-mucinous colorectal cancer.</p

Association of REG4 expression with other biomarkers in colorectal cancer and non-mucinous colorectal cancer.

<p>Exact pearson chi-square test for 2×2 tables. Missing data excluded from analyses.</p><p>Association of REG4 expression with other biomarkers in colorectal cancer and non-mucinous colorectal cancer.</p

REG4 expression in primary tumors and lymph-node metastases.

<p>REG4-negative expression in tumor and -positive in adjacent epithelium (A). Positive REG4 expression in primary tumor (B) and negative in its corresponding lymph node metastasis (C).Positive REG4 expression in primary tumor (D) and positive in its corresponding lymph node metastasis (E). Original magnification x 4 in figure A and x 20 in B–E.</p

Specificity of the <i>in situ</i> hybridization probe.

<p>PUUV RNA was detected by <i>in situ</i> hybridization, and visualized with diaminobenzidine in PUUV-infected Vero E6 cells (A), while mock-infected cells (B) showed no signal. PUUV N antigen was detected by immunohistochemistry, visualized by diaminobenzidine, in PUUV-infected cells (C), while no staining was seen in mock-infected cells (D).</p

Expression of AZIN2 in exocrine glands.

<p>The sweat-producing acinar epithelium of a normal sweat gland shows strong AZIN2 expression while the ductal cells (upper right corner) are negative (a). Lobular and ductal epithelium in non-lactating breast shows a weak reactivity (b). Tear gland epithelium shows AZIN2 positivity (c) in a distribution similar to that seen for Rab27b (d).</p

Detection of Puumala virus RNA and inflammatory cells in tissue samples

*<p>RT-PCR amplification of PUUV RNA was positive for all blood and tissue samples, except for the lung tissue samples of the monkeys #25 and #53</p>**<p>Antigen scoring: − = no positive cells, + = rare positive cells, ++ = moderate number of positive cells, +++ = many positive cells</p

HE staining shows lymphoid cells (arrows) in the kidney tissue of the monkey #53.

<p>Scale bar corresponds to 500 µm. The magnification shows a damaged tubulus surrounded by inflammatory cells.</p

Detection of PUUV genome.

<p>PUUV S segment RNA was detected by <i>in situ</i> hybridization, and visualized with Ventana Blue in the kidney (A), spleen (B), and liver (C) of the monkey #59. In the spleen, PUUV RNA signal was mostly endothelial (capillaries) with some positive dendritic cells (B), and in the liver samples PUUV RNA was found in Kupfer cells (C). Scale bar corresponds to 50 µm. Negative control tissues were stained in parallel to the infected monkeys. No signal was detected in the kidney (D), spleen (E), or liver (F) of the negative control monkey.</p

REG4 expression indicating better prognosis in non-mucinous colorectal cancer.

<p>Disease-specific survival analysis according to the Kaplan-Meier method for REG4 expression in non-mucinous colorectal cancer by the log-rank test.</p

Cox uni-and multivariable analysis of relative risk of death from non-mucinous colorectal cancer within 5 years by REG4 expression for patients under 65.

<p>Abbreviations: CI = confidence interval, HR = hazard ratio. Multivariable analysis included adjustment for gender, Dukes class, differentiation grade (G1/2 vs G3/4).</p><p>Cox uni-and multivariable analysis of relative risk of death from non-mucinous colorectal cancer within 5 years by REG4 expression for patients under 65.</p