314 research outputs found
Blood Clot Formation under Flow: The Importance of Factor XI Depends Strongly on Platelet Count
AbstractA previously validated mathematical model of intravascular platelet deposition and tissue factor (TF)-initiated coagulation under flow is extended and used to assess the influence on thrombin production of the activation of factor XI (fXI) by thrombin and of the activation of factor IX (fIX) by fXIa. It is found that the importance of the thrombin-fXIa-fIXa feedback loop to robust thrombin production depends on the concentration of platelets in the blood near the injury. At a near-wall platelet concentration of βΌ250,000/ΞΌL, typical in vessels in which the shear rate is <200 sβ1, thrombin activation of fXI makes a significant difference only at low densities of exposed TF. If the near-wall platelet concentration is significantly higher than this, either because of a higher systemic platelet count or because of the redistribution of platelets toward the vessel walls at high shear rates, then thrombin activation of fXI makes a major difference even for relatively high densities of exposed TF. The model predicts that the effect of a severe fXI deficiency depends on the platelet count, and that fXI becomes more important at high platelet counts
Late debut undifferentiated forms of spinal amyotrophy (clinical observation)
The clinical observation of a patient with undifferentiated form of spinal amiotrofii awards as monomelic form of spinal degeneration.Π ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΌ Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΠΈ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ ΠΏΠ°ΡΠΈΠ΅Π½Ρ Ρ Π½Π΅Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΡΠΎΡΠΌΠΎΠΉ ΡΠΏΠΈΠ½Π°Π»ΡΠ½ΠΎΠΉ Π°ΠΌΠΈΠΎΡΡΠΎΡΠΈΠΈ, Π΄Π΅Π±ΡΡΠΈΡΠΎΠ²Π°Π²ΡΠ΅ΠΉ ΠΊΠ°ΠΊ ΠΌΠΎΠ½ΠΎΠΌΠ΅Π»ΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠΎΡΠΌΠ° ΡΠΏΠΈΠ½Π°Π»ΡΠ½ΠΎΠΉ Π΄Π΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈ
Variaçáes nos métodos de aplicação dos herbicidas Diuron e Tripluralin na cultura do algodoeiro
Π Π°ΡΡΡΡΠΎΠΉΡΡΠ²Π° Π»ΠΈΠΏΠΈΠ΄Π½ΠΎΠ³ΠΎ ΠΎΠ±ΠΌΠ΅Π½Π° ΠΏΡΠΈ ΡΡΠΆΠ΅Π»ΠΎΠΌ ΡΠ΅ΠΏΡΠΈΡΠ΅: ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅ ΠΈ Π½ΠΎΠ²ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ ΠΊΠΎΡΡΠ΅ΠΊΡΠΈΠΈ
Objective: to reveal the basic regularities in the development of lipid metabolic disturbances in severe sepsis and to evaluate the efficiency of parenteral use of new balanced lipid emulsions in this cohort of patients. Subjects and methods. A prospective study was conducted in 88 patients with severe sepsis of different etiologies in the intensive care unit (ICI), Sverdlovsk Regional Clinical Hospital One. Among the lipid metabolic parameters, serum cholesterol, triglycerides (TG), high-density lipoproteins, low-density lipoproteins, and atherogenicity index were measured. Out of the systemic inflammatory markers and the additional criteria for sepsis severity, the serum levels of C-reactive protein, nitric oxide, lactate, D-dimers, the anti-inflammatory cytokine IL-4 and the proinflammatory cytokine IL-8 were determined. Serum was taken on days 1, 3, 5, and 7 after admission to the ICI. The quantitative attributes were comparatively analyzed by the statistical program Β«Statistica 6.0Β». Results. The severity assessed by the APACHE II scale, the degree of multiple organ failures (MOF) evaluated by the SOFA scale, and lung lesion according to the MURREY scale were found to be closely related to the baseline serum TG levels in patients with severe sepsis. The findings suggest that patients with high TG levels have much higher resuscitative mortality rates. The clinical evaluation of the efficiency of the new method for correction of lipid metabolic disturbances in severe sepsis has indicated that the patients receiving balanced (omega-3 fatty acids-enriched) fat emulsions as 20% Lipoplus solution had lower APACHE II scores within the first 7 days of intensive therapy and significantly more positive SOFA MOF changes. Specific changes were revealed in the presence of systemic inflammatory markers, such as C-reactive protein, IL-8, and IL-4, which confirms that balanced lipid emulsions are able to affect the system of pro- and anti-inflammatory mediators. Conclusion. The baseline increased serum TG level may be regarded as an additional criterion for the severity of sepsis. The new-generation balanced fat emulsions used in the parenteral feeding program for patients with severe sepsis can reduce the severity of their condition and the manifestations of MOF and affect the system of pro- and anti-inflammatory mediators. Key words: severe sepsis, lipid metabolic disturbances, balanced lipid emulsions.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ β Π²ΡΡΠ²ΠΈΡΡ ΠΎΡΠ½ΠΎΠ²Π½ΡΠ΅ Π·Π°ΠΊΠΎΠ½ΠΎΠΌΠ΅ΡΠ½ΠΎΡΡΠΈ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΡΠ°ΡΡΡΡΠΎΠΉΡΡΠ² Π»ΠΈΠΏΠΈΠ΄Π½ΠΎΠ³ΠΎ ΠΎΠ±ΠΌΠ΅Π½Π° ΠΏΡΠΈ ΡΡΠΆΠ΅Π»ΠΎΠΌ ΡΠ΅ΠΏΡΠΈΡΠ΅ ΠΈ ΠΎΡΠ΅Π½ΠΈΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΏΠ°ΡΠ΅Π½ΡΠ΅ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ Π½ΠΎΠ²ΡΡ
ΡΠ±Π°Π»Π°Π½ΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π»ΠΈΠΏΠΈΠ΄Π½ΡΡ
ΡΠΌΡΠ»ΡΡΠΈΠΉ Ρ Π΄Π°Π½Π½ΠΎΠΉ ΠΊΠ°ΡΠ΅Π³ΠΎΡΠΈΠΈ Π±ΠΎΠ»ΡΠ½ΡΡ
. ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΏΡΠΎΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Ρ 88 Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ ΡΡΠΆΠ΅Π»ΡΠΌ ΡΠ΅ΠΏΡΠΈΡΠΎΠΌ ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠΉ ΡΡΠΈΠΎΠ»ΠΎΠ³ΠΈΠΈ Π² ΠΠ ΠΠ’ Π‘Π²Π΅ΡΠ΄Π»ΠΎΠ²ΡΠΊΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠ½ΠΎΠΉ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π±ΠΎΠ»ΡΠ½ΠΈΡΡ β1. ΠΠ· ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ Π»ΠΈΠΏΠΈΠ΄Π½ΠΎΠ³ΠΎ ΠΎΠ±ΠΌΠ΅Π½Π° ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈΡΡ Ρ
ΠΎΠ»Π΅ΡΡΠ΅ΡΠΈΠ½ ΠΈ ΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄Ρ (Π’Π) Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ, Π° ΡΠ°ΠΊΠΆΠ΅ Π»ΠΈΠΏΠΎΠΏΡΠΎΡΠ΅ΠΈΠ΄Ρ Π²ΡΡΠΎΠΊΠΎΠΉ ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΠΈ, Π½ΠΈΠ·ΠΊΠΎΠΉ ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΠΈ ΠΈ ΠΈΠ½Π΄Π΅ΠΊΡ Π°ΡΠ΅ΡΠΎΠ³Π΅Π½Π½ΠΎΡΡΠΈ. ΠΠ· ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² ΡΠΈΡΡΠ΅ΠΌΠ½ΠΎΠ³ΠΎ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΡ ΠΈ Π΄ΠΎΠΏΠΎΠ»Π½ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΠΊΡΠΈΡΠ΅ΡΠΈΠ΅Π² ΡΡΠΆΠ΅ΡΡΠΈ ΡΠ΅ΠΏΡΠΈΡΠ° ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΡΡΠ²ΠΎΡΠΎΡΠΎΡΠ½ΡΠ΅ ΡΡΠΎΠ²Π½ΠΈ Π‘-ΡΠ΅Π°ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ°, ΠΎΠΊΡΠΈΠ΄Π° Π°Π·ΠΎΡΠ°, Π»Π°ΠΊ-ΡΠ°ΡΠ°, Π-Π΄ΠΈΠΌΠ΅ΡΠΎΠ², Π°Π½ΡΠΈΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΈΡΠΎΠΊΠΈΠ½Π° IL-4 ΠΈ ΠΏΡΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΈΡΠΎΠΊΠΈΠ½Π° IL-8. ΠΠ°Π±ΠΎΡ ΡΡΠ²ΠΎΡΠΎΡΠΊΠΈ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π½Π° 1-Π΅, 3-ΠΈ, 5-Π΅ ΠΈ 7-Π΅ ΡΡΡΠΊΠΈ ΠΎΡ ΠΌΠΎΠΌΠ΅Π½ΡΠ° ΠΏΠΎΡΡΡΠΏΠ»Π΅Π½ΠΈΡ Π² ΠΠ ΠΠ’. Π‘ΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΡ Β«Statistica 6.0Β». Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΡΡΠ²Π»Π΅Π½Π° ΡΠ΅ΡΠ½Π°Ρ Π²Π·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·Ρ ΠΌΠ΅ΠΆΠ΄Ρ ΡΡΠΆΠ΅ΡΡΡΡ ΡΠΎΡΡΠΎΡΠ½ΠΈΡ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² ΠΏΠΎ ΡΠΊΠ°Π»Π΅ APACHE II, ΡΡΠ΅ΠΏΠ΅Π½ΡΡ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΡΡΠΈ ΠΠΠ ΠΏΠΎ ΡΠΊΠ°Π»Π΅ SOFA ΠΈ ΡΡΠ΅ΠΏΠ΅Π½ΡΡ Π»Π΅Π³ΠΎΡΠ½ΠΎΠ³ΠΎ ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½ΠΈΡ ΠΏΠΎ ΡΠΊΠ°Π»Π΅ MURREY c ΠΈΡΡ
ΠΎΠ΄Π½ΡΠΌ ΡΡΠΎΠ²Π½Π΅ΠΌ ΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄ΠΎΠ² ΡΡΠ²ΠΎΡΠΎΡΠΊΠΈ ΠΊΡΠΎΠ²ΠΈ Ρ Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ ΡΡΠΆΠ΅Π»ΡΠΌ ΡΠ΅ΠΏΡΠΈΡΠΎΠΌ. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ Π²ΡΡΠΎΠΊΠΎΠΉ ΡΠ΅Π°Π½ΠΈΠΌΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π»Π΅ΡΠ°Π»ΡΠ½ΠΎΡΡΠΈ Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π²ΡΡΠΎΠΊΠΈΠΌ ΡΡΠΎΠ²Π½Π΅ΠΌ Π’Π. ΠΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΎΡΠ΅Π½ΠΊΠ° ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π½ΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠΎΠ΄Π° ΠΊΠΎΡΡΠ΅ΠΊΡΠΈΠΈ ΡΠ°ΡΡΡΡΠΎΠΉΡΡΠ² Π»ΠΈΠΏΠΈΠ΄Π½ΠΎΠ³ΠΎ ΠΎΠ±ΠΌΠ΅Π½Π° ΠΏΡΠΈ ΡΡΠΆΠ΅Π»ΠΎΠΌ ΡΠ΅ΠΏΡΠΈΡΠ΅ ΠΏΠΎΠΊΠ°Π·Π°Π»Π°, ΡΡΠΎ Ρ Π±ΠΎΠ»ΡΠ½ΡΡ
, ΠΏΠΎΠ»ΡΡΠ°Π²ΡΠΈΡ
ΡΠ±Π°Π»Π°Π½ΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ (ΠΎΠ±ΠΎΠ³Π°ΡΠ΅Π½Π½ΡΠ΅ ΠΎΠΌΠ΅Π³Π°-3 ΠΆΠΈΡΠ½ΡΠΌΠΈ ΠΊΠΈΡΠ»ΠΎΡΠ°ΠΌΠΈ), ΠΆΠΈΡΠΎΠ²ΡΠ΅ ΡΠΌΡΠ»ΡΡΠΈΠΈ Π² Π²ΠΈΠ΄Π΅ 20% ΡΠ°ΡΡΠ²ΠΎΡΠ° ΠΠΈΠΏΠΎΠΏΠ»ΡΡΠ° Π²ΡΡΠ²Π»Π΅Π½Ρ Π½ΠΈΠ·ΠΊΠΈΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ ΡΡΠΆΠ΅ΡΡΠΈ ΡΠΎΡΡΠΎΡΠ½ΠΈΡ ΠΏΠΎ APACHE II Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΏΠ΅ΡΠ²ΡΡ
7 ΡΡΡΠΎΠΊ ΠΈΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΈ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎ ΠΏΠΎΠ·ΠΈΡΠΈΠ²Π½Π°Ρ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠ° ΠΠΠ ΠΏΠΎ ΡΠΊΠ°Π»Π΅ SOFA. ΠΡΡΠ²Π»Π΅Π½Π° ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠ°Ρ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠ° ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² ΡΠΈΡΡΠ΅ΠΌΠ½ΠΎΠ³ΠΎ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΡ: Π‘-ΡΠ΅Π°ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΠΈΠ½Π°, IL-8 ΠΈ IL-4 β ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π°ΡΡΠ°Ρ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ Π½Π° ΡΠΈΡΡΠ΅ΠΌΡ ΠΏΡΠΎ- ΠΈ Π°Π½ΡΠΈΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΠΌΠ΅Π΄ΠΈΠ°ΡΠΎΡΠΎΠ² Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΠ±Π°Π»Π°Π½ΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π»ΠΈΠΏΠΈΠ΄Π½ΡΡ
ΡΠΌΡΠ»ΡΡΠΈΠΉ. ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΡΡ
ΠΎΠ΄Π½ΠΎ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΡΠΉ ΡΡΠΎΠ²Π΅Π½Ρ Π’Π ΡΡΠ²ΠΎΡΠΎΡΠΊΠΈ ΠΊΡΠΎΠ²ΠΈ ΠΌΠΎΠΆΠ½ΠΎ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡ ΠΊΠ°ΠΊ Π΄ΠΎΠΏΠΎΠ»Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ ΠΊΡΠΈΡΠ΅ΡΠΈΠΉ ΡΡΠΆΠ΅ΡΡΠΈ ΡΠ΅ΠΏΡΠΈΡΠ°. ΠΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΡΠ±Π°Π»Π°Π½ΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΆΠΈΡΠΎΠ²ΡΡ
ΡΠΌΡΠ»ΡΡΠΈΠΉ Π½ΠΎΠ²ΠΎΠ³ΠΎ ΠΏΠΎΠΊΠΎΠ»Π΅Π½ΠΈΡ Π² ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠ΅ ΠΏΠ°ΡΠ΅Π½ΡΠ΅ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΠΈΡΠ°Π½ΠΈΡ Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ ΡΡΠΆΠ΅Π»ΡΠΌ ΡΠ΅ΠΏΡΠΈΡΠΎΠΌ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΡΠΌΠ΅Π½ΡΡΠΈΡΡ ΡΡΠΆΠ΅ΡΡΡ ΡΠΎΡΡΠΎΡΠ½ΠΈΡ ΠΈ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΡΡΡ ΠΠΠ, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΎΠΊΠ°Π·ΡΠ²Π°Π΅Ρ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ Π½Π° ΡΠΈΡΡΠ΅ΠΌΡ ΠΏΡΠΎ- ΠΈ Π°Π½ΡΠΈΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΠΌΠ΅Π΄ΠΈΠ°ΡΠΎΡΠΎΠ². ΠΠ»ΡΡΠ΅Π²ΡΠ΅ ΡΠ»ΠΎΠ²Π°: ΡΡΠΆΠ΅Π»ΡΠΉ ΡΠ΅ΠΏΡΠΈΡ, ΡΠ°ΡΡΡΡΠΎΠΉΡΡΠ²Π° Π»ΠΈΠΏΠΈΠ΄Π½ΠΎΠ³ΠΎ ΠΎΠ±ΠΌΠ΅Π½Π°, ΡΠ±Π°Π»Π°Π½ΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΆΠΈΡΠΎΠ²ΡΠ΅ ΡΠΌΡΠ»ΡΡΠΈΠΈ
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Regulation of early steps of GPVI signal transduction by phosphatases: a systems biology approach
We present a data-driven mathematical model of a key initiating step in platelet activation, a central process in the prevention of bleeding following Injury. In vascular disease, this process is activated inappropriately and causes thrombosis, heart attacks and stroke. The collagen receptor GPVI is the primary trigger for platelet activation at sites of injury. Understanding the complex molecular mechanisms initiated by this receptor is important for development of more effective antithrombotic medicines. In this work we developed a series of nonlinear ordinary differential equation models that are direct representations of biological hypotheses surrounding the initial steps in GPVI-stimulated signal transduction. At each stage model simulations were compared to our own quantitative, high-temporal experimental data that guides further experimental design, data collection and model refinement. Much is known about the linear forward reactions within platelet signalling pathways but knowledge of the roles of putative reverse reactions are poorly understood. An initial model, that includes a simple constitutively active phosphatase, was unable to explain experimental data. Model revisions, incorporating a complex pathway of interactions (and specifically the phosphatase TULA-2), provided a good description of the experimental data both based on observations of phosphorylation in samples from one donor and in those of a wider population. Our model was used to investigate the levels of proteins involved in regulating the pathway and the effect of low GPVI levels that have been associated with disease. Results indicate a clear separation in healthy and GPVI deficient states in respect of the signalling cascade dynamics associated with Syk tyrosine phosphorylation and activation. Our approach reveals the central importance of this negative feedback pathway that results in the temporal regulation of a specific class of protein tyrosine phosphatases in controlling the rate, and therefore extent, of GPVI-stimulated platelet activation
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