9 research outputs found
Lavagem traqueobrĂ´nquica por sondagem nasotraqueal em bezerros Tracheobronchial lavage in calves using a nasotracheal technique
Avaliou-se a tĂ©cnica de lavagem traqueobrĂ´nquica por sondagem nasotraqueal e caracterizou-se a população celular em 10 bezerros clinicamente sadios. ApĂłs a contenção dos animais em decĂşbito lateral e auxĂlio de sonda guia, foi introduzida uma sonda de menor diâmetro atĂ© a bifurcação da traquĂ©ia, para produzir tosse e obter o lavado traqueobrĂ´nquico. A mĂ©dia de cĂ©lulas totais nas amostras de lavado foi de 133.750 cĂ©lulas/ml. Ă€ citologia, foram observados na contagem diferencial: 77,2% macrĂłfagos, 14,9% cĂ©lulas epiteliais cilĂndricas, 6,0% neutrĂłfilos e 1,8% linfĂłcitos. Das cĂ©lulas epiteliais cilĂndricas, 79,0% eram do tipo ciliadas e 21,0% nĂŁo-ciliadas. A mĂ©dia de contagem de macrĂłfagos binucleados foi de 78,5 cĂ©lulas/lâmina, a de macrĂłfagos trinucleados de 20,5/lâmina e a de cĂ©lulas gigantes 28,5/lâmina. Concluiu-se que o mĂ©todo de colheita por sondagem nasotraqueal Ă© eficiente para caracterizar a citologia do lavado traqueobrĂ´nquico de bezerros clinicamente sadios.<br>Tracheobronchial lavage through nasotracheal via was performed in 10 clinically health calves. They were maintained in lateral recumbence to perform the procedure. A small tube inserted into a guide tube was introduced until the tracheal bifurcation, producing cough, facilitating the collection of the lavage fluid. The mean number of total cells present in the samples was 133,750 cells/ml. The differential counting was represented by 77.2% of macrophages, 14.9% of cylindrical epithelial cells, 6.0% of neutrophils, 1.8% of lymphocytes. The cylindrical ciliated cells represented 79.0% of the sample and the nonciliated cells represented 21.0%. The mean number of macrophages was 78.5 of binucleated cells, 20.5 of trinucleated cells, and 28.5 of giant cells per smear. The tracheobronchial lavage obtained by this technique was an efficient method to characterize the cytological population of the lungs of clinically health calves
Interactions of Staphylococci with Osteoblasts and Phagocytes in the Pathogenesis of Implant-Associated Osteomyelitis
In spite of great advancements in the field of biomaterials and in surgical techniques, the implant of medical devices is still associated with a high risk of bacterial infection. Implant-associated osteomyelitis is a deep infection of bone around the implant. The continuous inflammatory destruction of bone tissues characterizes this serious bone infectious disease. Staphylococcus aureus and Staphylococcus epidermidis are the most prevalent etiologic agents of implant-associated infections, together with the emerging pathogen Staphylococcus lugdunensis. Various interactions between staphylococci, osteoblasts, and phagocytes occurring in the pen-prosthesis environment play a crucial role in the pathogenesis of implant-associated osteomyelitis. Here we focus on two main events: internalization of staphylococci into osteoblasts, and bacterial interactions with phagocytic cells