Detecting and quantifying stress granules in tissues of multicellular organisms with the Obj.MPP analysis tool

International audienceStress Granules (SGs) are macromolecular assemblies induced by stress and composed of proteins and mRNAs stalled in translation initiation. SGs play an important role in the response to stress and in the modulation of signaling pathways. Furthermore, these structures are related to the pathological ribonucleoprotein (RNP) aggregates found in neurodegenerative disease contexts, highlighting the need to understand how they are formed and recycled in normal and pathological contexts. Although genetically tractable multicellular organisms have been key in identifying modifiers of RNP aggregate toxicity, in vivo analysis of SG properties and regulation has lagged behind, largely due to the difficulty of detecting SG from images of intact tissues. Here, we describe the object detector software Obj.MPP and show how it overcomes the limits of classical object analyzers to extract the properties of SGs from wide-field and confocal images of respectively C. elegans and Drosophila tissues. We demonstrate that Obj.MPP enables the identification of genes modulating the assembly of endogenous and pathological SGs, and thus that it will be useful in the context of future genetic screens and in vivo studies. This article is protected by copyright. All rights reserved

Linear and nonlinear Biot waves in a noncohesive granular medium slab: Transfer function, self-action, second harmonic generation

International audienc

Non Destructive Evaluation of concrete material properties for nuclear power plant survey: results in the laboratory and on the VeRCoRS mock-up

NDE/NDT for Highways & Bridges: SMT 2018, NEWAK, ETATS-UNIS, 27-/08/2018 - 29/06/2018Pre-stressed concrete nuclear power plant containment vessel is the third barrier in case of an accident so that quantifying concrete properties with Non Destructive Evaluation (NDE) is a continuing goal. This includes the mapping of the following concrete properties: elastic modulus, water saturation, permeability. In addition, those measurements have to be challenged with the level of stress (which will be modified during an accident) possibly coupled with thermal induced damage. In the ANR French project 'Non Destructive Evaluation of containment nuclear plant structures' (ENDE) eight partners have carried out laboratory and in situ NDE measurements with the aim to compare and combine them to reach quantitative useful information about concrete conditions. In this paper we present a synthesis of the mains results obtained with classical and non-classical NDE methods. This includes electromagnetic techniques such as radar, capacitive measurements, resistivity measurements and ultrasonic measurements (impact echo, ultrasonic pulse velocity - in reflexion and transmission mode for longitudinal and transverse waves, surface waves, diffuse waves, coda wave interferometry, nonlinear acoustics, etc.). The laboratory experiments have been conducted on slabs 0.5 m x 0.25 m x 0.12 m, for one concrete mix formulated to be representative of nuclear containment walls, in different conditions: - sound concrete without any stress for different water saturations,- heat damage of partially saturated slab (at 80°C, 150°C, 200°C),- sound concrete under stress (up to 30% of the compressive strength),- heat damage concrete under stress.A series of NDE method combinations (using data fusion approach) is performed to solve the problem of multiple sensitivity issues. This laboratory work is then transposed on the VeRCoRS mock-up which is a 1/3 scaled concrete power plant (a Õ 16 m x h 30 m cylinder, with 0.40 m thick walls) heavily instrumented (700 sensors, 2 km of fiber optic). In this paper we present results obtained before, during and after a decennial test simulation which consists in increasing the internal pressure with air, maintaining its level to 4 bars during 2 hours and then decreasing back to the atmospheric pressure level.To conclude industrial and research perspectives are presented

The human remains from Axlor (Dima, Biscay, northern Iberian Peninsula)

OBJECTIVES We provide the description and comparative analysis of all the human fossil remains found at Axlor during the excavations carried out by J. M. de Barandiarán from 1967 to 1974: a cranial vault fragment and eight teeth, five of which likely belonged to the same individual, although two are currently lost. Our goal is to describe in detail all these human remains and discuss both their taxonomic attribution and their stratigraphic context. MATERIALS AND METHODS We describe external and internal anatomy, and use classic and geometric morphometrics. The teeth from Axlor are compared to Neandertals, Upper Paleolithic, and recent modern humans. RESULTS Three teeth (a left dm2, a left di1, and a right I1) and the parietal fragment show morphological features consistent with a Neandertal classification, and were found in an undisturbed Mousterian context. The remaining three teeth (plus the two lost ones), initially classified as Neandertals, show morphological features and a general size that are more compatible with their classification as modern humans. DISCUSSION The combined anatomical and stratigraphic study suggest that the remains of two different adult Neandertals have been recovered during the old excavations performed by Barandiarán: a left parietal fragment (Level VIII) and a right I1 (Level V). Additionally, two different Neandertal children lost deciduous teeth during the formations of levels V (left di1) and IV (right dm2). In addition, a modern human individual is represented by five remains (two currently lost) from a complex stratigraphic setting. Some of the morphological features of these remains suggest that they may represent one of the scarce examples of Upper Paleolithic modern human remains in the northern Iberian Peninsula, which should be confirmed by direct dating