558 research outputs found
Secondary Use Provisions in the European Health Data Space Proposal and Policy Recommendations for Korea
Objectives This article explores the secondary use provisions of the European Health Data Space (EHDS), proposed by the European Commission in May 2022, and offers policy recommendations for South Korea. Methods The authors analyzed the texts of the EHDS proposal and other documents published by the European Union, as well as surveyed the relevant literature. Results The EHDS proposal seeks to create new patient rights over electronic health data collected and used for primary care; and establish a data sharing system for the re-use of electronic health data for secondary purposes, including research, the provision of personalized healthcare, and developing healthcare artificial intelligence (AI) applications. These provisions envisage requiring both private and public data holders to share certain types of electronic health data on a mandatory basis with third parties. New government bodies, called health data access bodies, would review data access applications and issue data permits. Conclusions The overarching aim of the EHDS proposal is to make electronic health data, which are currently held in the hands of a small number of organizations, available for re-use by third parties to stimulate innovation and research. While it will be very challenging for South Korea to adopt a similar scheme and require private entities to share their proprietary data with third parties, the South Korean government should consider making at least health data collected through publicly funded research more readily available for secondary use
Preparative Synthesis of dTDP-L-Rhamnose Through Combined Enzymatic Pathways
dTDP-L-rhamnose, an important precursor of O-antigen, was prepared on a large scale from dTMP by executing an one-pot reaction in which six enzymes are involved. Two enzymes, dTDP-4-keto-6-deoxy-D-glucose 3,5-epimerase and dTDP-4-keto-rhamnose reductase, responsible for the conversion of dTDP-4-keto-6-deoxy- D-glucose to dTDP-L-rhamnose, were isolated from their putative sequences in the genome of Mesorhizobium loti, functionally expressed in Escherichia coli, and their enzymatic activities were identified. The two enzymes were combined with an enzymatic process for dTDP-4- keto-6-deoxy-D-glucose involving TMP kinase, acetate kinase, dTDP-glucose synthase, and dTDP-glucose 4,6- dehydratase, which allowed us to achieve a preparative scale synthesis of dTDP-L-rhamnose using dTMP and glucose-1-phosphate as starting materials. About 82% yield of dTDP-L-rhamnose was obtained based on initial dTMP concentration at 20 mM dTMP, 1 mM ATP, 10 mM NADH, 60 mM acetyl phosphate, and 80 mM glucose-1- phosphate. From the reaction with 20 ml volume, approximately 180 mg of dTDP-L-rhamnose was obtained in an overall yield of 60% after two-step purification, that is, anion exchange chromatography and gel filtration for desalting. The purified product was identifiedbyHPLC, ESI-MS,andNMR,showingabout95%purity
Characterization of GDP-mannose Pyrophosphorylase from Escherichia Coli O157:H7 EDL933 and Its Broad Substrate Specificity
GDP-mannose pyrophosphorylase gene (ManC) of Escherichia coli (E. coli) O157 was cloned and expressed as a highly soluble protein in E. coli BL21 (DE3). The enzyme was subsequently purified using hydrophobic and ion exchange chromatographies. ManC showed very broad substrate specificities for four nucleotides and various hexose-1-phosphates, yielding ADP-mannose, CDP-mannose, UDP-mannose, GDP-mannose, GDP-glucose and GDP-2-deoxy-glucose
Association between work-related health problems and job insecurity in permanent and temporary employees
OBJECTIVES: This research was conducted with an aim of determining the correlation between job insecurity and an employee’s work-related health problems among permanent and temporary workers. METHODS: Using the data from the First Korean Working Conditions Survey conducted in 2006, a total of 7,071 workers, excluding employers and the self-employed, were analyzed. Work-related health problems were categorized as backache, headache, abdominal pain, muscular pain, stress, fatigue, insomnia, anxiety or depression. Each problem was then analyzed for its relationship to job insecurity through logistic regression analysis. RESULTS: Among the 7,071 workers, 5,294 (74.9%) were permanent workers and 1,777 (25.1%) were temporary workers. For the permanent workers, presence of high or moderate job insecurity appeared more closely linked to backache, headache, abdominal pain, muscular pain, stress, fatigue, insomnia, anxiety, and depression compared to absence of job insecurity. However, for the temporary workers, only depression appeared to be associated with the presence of high job insecurity. CONCLUSION: The study showed that the presence of job insecurity is correlated with work-related health problems. The deleterious effects of job insecurity appeared to be stronger in permanent than temporary workers. Additional research should investigate ways to effectively reduce job insecurity
An OFDMA-Based Next-Generation Wireless Downlink System Design with Hybrid Multiple Access and Frequency Grouping Techniques
This paper discusses how to effectively design a nextgeneration
wireless communication system that can possibly provide
very high data-rate transmissions and versatile quality services.
In order to accommodate the sophisticated user requirements
and diversified user environments of the next-generation systems,
it should be designed to take an efficient and flexible structure
for multiple access and resource allocation. In addition, the design
should be optimized for cost-effective usage of resources and
for efficient operation in a multi-cell environment. As orthogonal
frequency division multiple access (OFDMA) has turned out in
recent researches to be one of the most promising multiple access
techniques that can possibly meet all those requirements through
efficient radio spectrum utilization, we take OFDMA as the basic
framework in the next-generation wireless communications system
design.
So, in this paper, we focus on introducing an OFDMA-based
downlink system design that employs the techniques of hybrid multiple
access (HMA) and frequency group (FG) in conjunction with
intra-frequency group averaging (IFGA). The HMA technique
combines various multiple access schemes on the basis of OFDMA
system, adopting the multiple access scheme that best fits to the
given user condition in terms of mobility, service, and environment.
The FG concept and IFGA technique help to reduce the feedback
overhead of OFDMA system and the other-cell interference (OCI)
problem by grouping the sub-carriers based on coherence bandwidths
and by harmonizing the channel condition and OCI of the
grouped sub-carriers.This work was supported in part by Samsung Advanced Institute of Technology
Cultural Practices to Reduce Cd Content in Edible Parts of Staple Crops in Korea
Objectives of this study were to determine the uptake and translocation of Cd in rice plant from soil with applying the water management and soil ameliorators and to investigate the correlations among heavy metal contents in the brown rice, soil pH and chemical species of Cd existing in soil by sequential extracting method with paddy soil contaminated with Cd near abandoned mine. To identify the effect of soil ameliorators on Cd uptake in rice plants, compost and lime were treated. Plants were grown with irrigation water concentrated by 0.01mg kg^ of cadmium in two soil types (sandy loam and clay loam) with treatments of intermittent irrigation and continuous submersion conditions. Compared to intermittent irrigation plots, average Eh value in the continuous submersion plots was low at 136.7mV whereas pH value was high at 0.3. Eh value was decreased in the treatment of soil ameliorator while pH value was increased by 0.2~0.3. Cd content of leaves and brown rice had significantly positive correlation with Eh value in soils while was negatively correlated with soil pH. At the harvest stage, Cd content in the leaves and brown rice was decreased in the continuous submersion plots by 30% relative to the intermittent irrigation plots. In case of soil ameliorator applied plots, Cd content of leaves and brown rice was lower by 35% than that of N, P, K fertilizer plots, respectively. Compared to the soil types, Cd content of leaves and brown rice in sandy loam soil was lower by 64 and 37% than that in clay loam soil, respectively. Order of reduction to Cd uptake was the compost and lime mixture plot>silicate plot>lime plot. However, the effect of Cd uptake reduction by soil ameliorator was decreased in the N, P, K+compost and N, P, K+phosphate plots. Cd uptake reduction by water management and soil ameliorator was more effective in the sandy loam soil than that in the clay loam soil.Special Revie
Analysis of Yield of Eleutherosides B and E in Acanthopanax divaricatus
An analysis of the yield of eleutherosides B and E in Acanthopanax divaricatus and A. koreanum was performed using high performance liquid chromatography to evaluate production by different cultivation methods. In A. divaricatus and A. koreanum, the total content of eleutherosides B and E was 2.466–7.360 mg/g varying by plant section, 3.886–11.506 mg/g by pinching site, 3.655–10.083 mg/g by planting time, and 3.652–10.108 mg/g by fertilizer ratio. Thus the total content of eleutherosides B and E in A. divaricatus and A. koreanum differed depending on cultivation methods. These results present useful information for high eleutheroside content applications in A. divaricatus and A. koreanum. This information can affect selection of plant section and cultivation methods for nutraceutical, pharmaceutical, and cosmeceutical material development
Protective humoral immune response induced by an inactivated porcine reproductive and respiratory syndrome virus expressing the hypo-glycosylated glycoprotein 5
Porcine reproductive and respiratory syndrome (PRRS) causes significant economic losses to the swine industry worldwide. Although inactivated and live vaccines are commercially available for the control of PRRS, both types of vaccine have not always proven successful in terms of generating a protective immune response, particularly in the case of inactivated vaccines. In this study, we tested whether an inactivated vaccine could induce a humoral immune response to PRRS during a homologous challenge. Amino acid substitutions were introduced into glycoprotein (GP) 5 of the FL12 strain of the PRRS virus (PRRSV) using site-directed mutagenesis with a pFL12 infectious clone. The substitutions led to double deglycosylation in the putative glycosylation moieties on GP5. The mutant virus was subsequently inactivated with binary ethylenimine. The efficacy of the inactivated mutant virus was compared with that of the inactivated wild-type PRRSV. Only the inactivated mutant PRRSV induced serum neutralizing antibodies at six weeks post-vaccination. The group that was administered the inactivated mutant virus twice exhibited a significantly increased neutralizing antibody titer after a challenge with the virulent homologous strain and exhibited more rapid clearing of viremia compared to other groups, including the groups that were administered either the inactivated mutant or wild-type virus only once and the group that was administered the inactivated wild-type virus twice. Histopathological examination of lung tissue sections revealed that the group that was administered the inactivated mutant virus twice exhibited significantly thinner alveolar septa, whereas the thickness of the alveolar septa of the other groups were markedly increased due to lymphocyte infiltration. These results indicated that the deglycosylation of GP5 enhanced the immunogenicity of the inactivated mutant PRRSV and that twice administrations of the inactivated mutant virus conferred better protection against the homologous challenge. These findings suggest that the inactivated PRRSV that expresses a hypo-glycosylated GP5 is a potential inactivated vaccine candidate and a valuable tool for controlling PRRS for the swine industry
Protective humoral immune response induced by an inactivated porcine reproductive and respiratory syndrome virus expressing the hypo-glycosylated glycoprotein 5
Porcine reproductive and respiratory syndrome (PRRS) causes significant economic losses to the swine industry worldwide. Although inactivated and live vaccines are commercially available for the control of PRRS, both types of vaccine have not always proven successful in terms of generating a protective immune response, particularly in the case of inactivated vaccines. In this study, we tested whether an inactivated vaccine could induce a humoral immune response to PRRS during a homologous challenge. Amino acid substitutions were introduced into glycoprotein (GP) 5 of the FL12 strain of the PRRS virus (PRRSV) using site-directed mutagenesis with a pFL12 infectious clone. The substitutions led to double deglycosylation in the putative glycosylation moieties on GP5. The mutant virus was subsequently inactivated with binary ethylenimine. The efficacy of the inactivated mutant virus was compared with that of the inactivated wild-type PRRSV. Only the inactivated mutant PRRSV induced serum neutralizing antibodies at six weeks post-vaccination. The group that was administered the inactivated mutant virus twice exhibited a significantly increased neutralizing antibody titer after a challenge with the virulent homologous strain and exhibited more rapid clearing of viremia compared to other groups, including the groups that were administered either the inactivated mutant or wild-type virus only once and the group that was administered the inactivated wild-type virus twice. Histopathological examination of lung tissue sections revealed that the group that was administered the inactivated mutant virus twice exhibited significantly thinner alveolar septa, whereas the thickness of the alveolar septa of the other groups were markedly increased due to lymphocyte infiltration. These results indicated that the deglycosylation of GP5 enhanced the immunogenicity of the inactivated mutant PRRSV and that twice administrations of the inactivated mutant virus conferred better protection against the homologous challenge. These findings suggest that the inactivated PRRSV that expresses a hypo-glycosylated GP5 is a potential inactivated vaccine candidate and a valuable tool for controlling PRRS for the swine industry
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