291,870 research outputs found

    The Effect Of Peroxisome Proliferatorr Activated Receptor Gamma (Pparγ) On The Expression Of Foxp3, Tigit, Icos And Histone Proteins In Natural T-Regulatory Cells

    Get PDF
    Natural T Regulatory (nTreg) cells represent approximately 8-10% of the total CD4+ T cell population. These cells are crucial for immune homeostasis and autoimmunity prevention. Previous study showed that peroxisome proliferator-activated receptor gamma (PPARγ) ligands suppress Forkhead box P3 (FoxP3) expression in nTreg cells following 72 hours in vitro culture. Current study was performed to elucidate the effects of PPARγ ligands on T cell immunoreceptor with Ig and ITIM domains (TIGIT) and Inducible T cell costimulator (ICOS) expressions in activated nTreg cells isolated from Balb/c mice. We also focused on histone modifications on FoxP3 gene expression in activated nTreg cells following PPARγ ligand, 15-Deoxy-△(12,14)-prostaglandin J2 (15d-PGJ2) and its inhibitor, GW9662 treatment in type 1 diabetes (T1D) mouse model. Spleens of Balb/c, NOD and NOR mice were harvested through cervical dislocation. CD4+CD25+ cells were isolated using MoFlow automated sorter or Magnetic-activated cell sorting (MACS) magnetic separation and purity was analyzed by flow cytometry method. Isolated CD4+CD25+ cells were cultured for 72 hours in supplemented RPMI in the presence of anti-CD3/CD28 antibodies and IL-2 cytokine. In Balb/c mice, sorted cells treated with or without 15d-PGJ2 or ciglitazone for TIGIT and ICOS surface marker profiling. In NOD/NOR mice, isolated cells were treated with 15d-PGJ2 or ciglitazone with or without GW9662 inhibitor

    Antietam Playbill

    Get PDF
    Providence College Department of Theatre, Dance & Film Antietam, an American Opera Music & Libretto, Michael Dombrow & Joseph Sustar April 10-13, 1997 Director, Ann Garner Stage Manager, Laura Ann Chepiga Scenic/ Props Design, Carolyn J. Stokes Costume Design, Elizabeth A. Brady Lighting Design, Patrick W. Austin Cast: Mark T. Valahovic - Narrator; Elizabeth Benson - Elizabeth Yellin; Pete Mitchell - Michael Yellin; Richard Brewer - Phillip Teuffel; Michael Gariglio and Patrick Salvato - Southern Soldiers; Christina Eger and Denise Tracey - Southern Ladies; Shayna Ross - Mary; Matt Tahaney - Daniel; Jennifer E. Cuddy - Sarah; Craig Donnelly - Samuel; Michael Cuddy - Northern Soldier; Jaime Lee Babstock, Sharon McMahon, Laura Smith; Mark T. Valahovic - General McDowell; Craig Donnelly - Southern Soldier; Michael Gariglio - General Lee; Patrick Salvato - Lee\u27s Aide; Mark T. Valahovic - General McClellan; Michael Cuddy - McClellan\u27s Aidehttps://digitalcommons.providence.edu/promos_1990s_pubs/1000/thumbnail.jp

    Mandy Lee

    Full text link
    Date unknown.https://digitalcommons.georgefox.edu/levi_pennington/1360/thumbnail.jp

    The High Water Mark of Social History in Civil War Studies

    Full text link
    Just hours before the Army of Northern Virginia raised the white flag at Appomattox Court House, Confederate Colonel Edward Porter Alexander approached his commanding officer, Robert E. Lee, with what he hoped was a game-saving plan. Rather than suffer the mortification of surrendering, Alexander begged Lee to scatter his men across the countryside like “rabbits & partridges” where they could continue waging war, not as regular Confederate soldiers, but as elusive guerrilla fighters. Lee listened patiently to his subordinate’s reasoning for irregular warfare. Before Alexander finished, he reminded Lee that the men were utterly devoted to their commanding general, and that such loyalty would continue to inspire the sacrifice of more blood, even if it meant taking to the woods and fighting like common outlaws. When Alexander concluded his impassioned plea, Lee asked his subordinate to imagine what would happen if he turned Alexander’s suggestion into official policy. But before Alexander had a chance to respond, Lee reminded him that virtually every Southern community had been overrun by Union armies, that farms were in disarray, and that crops were ruined. Lee feared that his veterans, upon returning home, would have no choice but to plunder and rob for survival. It would take no time for his disciplined army to descend into a demoralized mob that would take the rest of the South into a downward spiral of unending and unrestrained violence. “As for myself,” Lee concluded, “while you young men might afford to go to bushwhacking, the only proper & dignified course for me would be to surrender myself & take the consequences of my actions.” [excerpt

    Auxiliary-Stranding Relative Clauses

    Get PDF
    A little discussed feature of English are non-restrictive relative clauses in which the antecedent is normally not an NP and the gap follows an auxiliary, as in Kim will sing, which Lee won?t. These relative clauses resemble clauses with auxiliary complement ellipsis or fronting. There are a variety of analyses that might be proposed, but there are reasons for thinking that the best analysis is one where which is a nominal filler associated with a gap which is generally non-nominal: a filler-gap mismatch analysis in other words
    corecore