2,703 research outputs found
Successful Use of Newspapers in Curriculum Development for First-year College Students
The course, Fashion and Society, is designed to provide first-year Clothing and Textiles major students with an integrated view of fashion as social phenomenon. From the survey on the first-year studentsā perceptions about fashion, we have found that many students considered fashion tend to be irrelevant, something fancy and distant to their daily life, and difficult to understand. Based on the comments, we need teaching materials that can be perceived to be ānot difficultā (and fun) and relevant. We also needed to design the course to provide students opportunities to apply their knowledge to real life. Thus, we developed the course using several newspapers to help students experience various fashion expressions and reflections a society carries in
Influence of cultivation parameters or supplement on product qualities and culture performances during perfusion
Perfusion processes have been developed with technological advances in single-use bioreactor and cell retention device. Perfusion has advantages such as high cell density culture in compact facilities and media change or culture parameter shifts for protein production with desired qualities. Single use bioreactor (200L or 1000L) with ATF System was used for the clinical production. For the process development, the scale-down model was established with lab-scale (2L) bioreactor with ATF2.
A recombinant CHO cell line producing a fusion protein was cultivated using in-house serum-free media. Influence of insulin (0~3mg/L) on qualities was investigated in the established perfusion process using in-house serum-free medium and 2L scale-down model. The results showed that low concentration of insulin enhanced O-glycosylation and -2 charged N-glycan of fusion protein. Temperature (30~34oC) conditions, under no addition of insulin, were investigated to evaluate effect on qualities. High temperature enhanced O-glycosylation and -2 charged N-glycan of fusion protein.
To evaluate interaction among culture parameters (Temperature, pH, and Dissolved Oxygen) in the perfusion process, Central Composite Inscribed (CCI) was selected as design of experiment. 20 perfusion cultures were carried out in the 2L scale-down model. The results showed each parameter and interactions among parameters had an effect on qualities and culture performances
Od pukog urbaniteta do urbanih bioetiÄkih standarda: poziv na Å”irenje bioetike
An interest in research, deliberation, and reflection on urbanity has been present for a long
time. Due to rapid urbanisation in the last few decades, such interest has intensified, attracting
scholars from different disciplines and creating new platforms for discussion.
The first indicators of a ābioethicalā interest in urban life are already present in Van Rensselaer
Potterās early papers (urban ethics. However, more extensive research into urban bioethics
remained on hold until recently, mainly due to the dominance of the biomedical paradigm
within modern mainstream bioethics.
In 2017, the European Bioethics in Action project (funded by the Croatian Science
Foundation) ended, resulting in a list of general bioethical standards related to animals,
plants, and human health. The aim of this paper is to present the rationale for developing
bioethical standards in a specific urban context.Interes za istraživanje, promiÅ”ljanje i promiÅ”ljanje urbanosti veÄ je duže vrijeme prisutan.
Posljednjih nekoliko desetljeÄa, zbog rapidne urbanizacije, taj se interes dodatno intenzivirao,
privlaÄeÄi znanstvenike razliÄitih disciplina i stvarajuÄi nove platforme rasprave. Prvi
pokazatelji ābioetiÄkogā interesa za urbani život prisutni su veÄ u ranim radovima Van
Rensselaera Pottera (urbana etika), no iscrpnija istraživanja urbane bioetike sve su donedavno
bila zanemarena, uglavnom kao posljedica dominacije biomedicinske paradigme unutar
bioetike. Jedan od rezultata nedavno provedenog projekta āEuropska bioetika na djelu
EuroBioActā (koji je financirala Hrvatska zaklada za znanost, 2014. - 2017.), bila je lista
opÄih bioetiÄkih standarda koji su definirali prihvatljiv odnos prema životinjama, biljkama
i zdravlju ljudi. NadovezujuÄi se na spomenuto, cilj ovog rada je razrada uporiÅ”ta za razvoj
bioetiÄkih standarda u specifiÄnom urbanom kontekstu
Observation of Competing Order in a High- Superconductor with Femtosecond Optical Pulses
We present studies of the photoexcited quasiparticle dynamics in
TlBaCaCuO (Tl-2223) using femtosecond optical
techniques. Deep into the superconducting state (below 40 K), a dramatic change
occurs in the temporal dynamics associated with photoexcited quasiparticles
rejoining the condensate. This is suggestive of entry into a coexistence phase
which, as our analysis reveals, opens a gap in the density of states (in
addition to the superconducting gap), and furthermore, competes with
superconductivity resulting in a depression of the superconducting gap.Comment: 5 pages, 3 figure
Hippocampal sclerosis and encephalomalacia as prognostic factors of tuberculous meningitis-related and herpes simplex encephalitis-related epilepsy
AbstractBackgroundTuberculous meningitis (TBM) and herpes simplex encephalitis (HSE) are common neurological diseases involving the brain parenchyma, and both can result in chronic epilepsy. Here, we identified possible variables affecting the prognosis of central nervous system (CNS) infection-related epilepsy.MethodsThe clinical seizure characteristics and demographic data of 20 TBM- and 55 HSE-related epilepsy patients were compared. Statistically significant prognostic variables were identified using multiple regression analysis.ResultsSex, age at infection, age at epilepsy onset, presence of seizures at the time of infection, latency period, and seizure characteristics between two groups were similar except for the pattern of brain lesions observed on the MRI and their overall prognosis. Patients with hippocampal sclerosis (HS) only comprised 30% and 52.7% of the TBM and HSE groups, respectively. Encephalomalacia had a positive effect in the HSE group while HS had a negative effect in this group, but no significant effects were found in the TBM group. Through a multiple regression analysis with a correction for group effects, HS was associated with a poor prognosis. However, encephalomalacia was concomitantly associated with a good prognosis. In addition, a short latency period, with a one-year interval, and being male were both associated with a good prognosis, while the age at the onset of epilepsy was associated with a poor prognosis.ConclusionsThis study suggests that HS and encephalomalacia could have mutual but contradictory effects on the prognosis of CNS infection-related epilepsy
Development of SCAR markers for the identification of Phytophthora katsurae causing chestnut ink disease in Korea
Sequence characterized amplified region (SCAR) markers are one of the most effective and accurate tools for microbial
identification. In this study, we applied SCAR markers for the rapid and accurate detection of Phytophthora katsurae, the casual
agent of chestnut ink disease in Korea. In this study, we developed seven SCAR markers specific to P. katsurae using random
amplified polymorphic DNA (RAPD), and assessed the potential of the SCAR markers to serve as tools for identifying P. katsurae.
Seven primer pairs (SOPC 1F/SOPC 1R, SOPC 1-1F/SOPC 1-1R, SOPC 3F/SOPC 3R, SOPC 4F/SOPC 4R, SOPC 4F/SOPC 4-1R,
SOPD 9F/SOPD 9R, and SOPD 10F/SOPD 10R) from a sequence derived from RAPD fragments were designed for the analysis
of the SCAR markers. To evaluate the specificity and sensitivity of the SCAR markers, the genomic DNA of P. katsurae was serially
diluted 10-fold to final concentrations from 1 mg/mL to 1 pg/mL. The limit of detection using the SCAR markers ranged from
100 Ī¼g/mL to 100 ng/mL. To identify the limit for detecting P. katsurae zoospores, each suspension of zoospores was serially
diluted 10-fold to final concentrations from 10 Ć 105 to 10 Ć 101 zoospores/mL, and then extracted. The limit of detection by
SCAR markers was approximately 10 Ć 101 zoospores/mL. PCR detection with SCAR markers was specific for P. katsurae, and did
not produce any P. katsurae-specific PCR amplicons from 16 other Phytophthora species used as controls. This study shows that
SCAR markers are a useful tool for the rapid and effective detection of P. katsurae.This research was supported from a Forest Science and
Technology Project (Project No. C1002315) provided
through the Korea Forest Service.http://www.mycobiology.or.kr/am201
Investigation on the calibration for the membrane type heat flux sensor using micro heater
This paper presents the calibration accuracy of the membrane type heat flux sensor using micro heater. The directional heat flow rate into the thermal sensor is simulated with FEM to estimate the heat flux from micro heater for calibration. Thin film thermopile which is fabricated on the dielectric membrane directly converts the temperature difference between hot and cold junctions of thermopile into heat flux signal created by the thermoelectric effect. However, symmetric design of thermopile is restricted to only measure x-directional heat flow rate, when heat also propagates through y direction via heat conduction and z direction with convection. Therefore, it is highly needed to investigate how amount of heat from the micro heater contributes to the calibration of heat flux sensor. In the case of the calibration using micro heater, heat conduction through the membrane dominates heat flow. Thus, 97% of heat flow from micro heater propagates through the thermopile and generates 1.038 V/W of sensitivity that was estimated by the simulated model as 1.066 V/W. On the other hand, in case of water filled micro channel, 87% of heat flow reaches to thermopile and estimated 0.392 V/W of sensitivity. This heat loss leads to the inaccurate calibration as well as the lowered sensitivity.Papers presented to the 12th International Conference on Heat Transfer, Fluid Mechanics and Thermodynamics, Costa de Sol, Spain on 11-13 July 2016
Optical repumping of triplet -states enhances magneto-optical trapping of ytterbium atoms
Radiative decay from the excited state to metastable and
states is expected to limit attainable trapped atomic population in a
magneto-optic trap of ytterbium (Yb) atoms. In experiments we have carried out
with optical repumping of states to , we observe enhancement
of trapped atoms yield in the excited state. The individual decay rate
to each metastable state is measured and the results show an excellent
agreement with the theoretical values.Comment: 5 pages, 5 figure
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