Complete Semiclassical Treatment of the Quantum Black Hole Problem

Two types of semiclassical calculations have been used to study quantum effects in black hole backgrounds, the WKB and the mean field approaches. In this work we systematically reconstruct the logical implications of both methods on quantum black hole physics and provide the link between these two approaches. Our conclusions completely support our previous findings based solely on the WKB method: quantum black holes are effectively p-brane excitations and, consequently, no information loss paradox exists in this problem.Comment: 14 pages, REVTE

Conjectures on Non-Local Effects in String Black Holes

We consider modifications to general relativity by the non-local (classical and quantum) string effects for the case of a D-dimensional Scwarzschild black hole. The classical non-local effects do not alter the spacetime topology (the horizon remains unshifted, at least perturbatively). We suggest a simple analytic continuation of the perturbative result into the non-perturbative domain, which eliminates the black hole singularity at the origin and yields an ultraviolet-finite theory of quantum gravity. We investigate the quantum non- local effects (including massive modes) and argue that the inclusion of these back reactions resolves the problem of the thermal spectrum in the semi- classical approach of field quantization in a black hole background, through the bootstrap condition. The density of states for both the quantum and thermal interpretation of the WKB formula are finally shown to differ quant- itatively when including the non-local effects.Comment: 16 pages, REVTE

Cranial and trunk neural crest cells use different mechanisms for attachment to extracellular matrices

We have used a quantitative cell attachment assay to compare the interactions of cranial and trunk neural crest cells with the extracellular matrix (ECM) molecules fibronectin, laminin and collagen types I and IV. Antibodies to the β_1 subunit of integrin inhibited attachment under all conditions tested, suggesting that integrins mediate neural crest cell interactions with these ECM molecules. The HNK-1 antibody against a surface carbohydrate epitope under certain conditions inhibited both cranial and trunk neural crest cell attachment to laminin, but not to fibronectin. An antiserum to α_1 intergrin inhibited attachment of trunk, but not cranial, neural crest cells to laminin and collagen type I, though interactions with fibronectin or collagen type IV were unaffected. The surface properties of trunk and cranial neural crest cells differed in several ways. First, trunk neural crest cells attached to collagen types I and IV, but cranial neural crest cells did not. Second, their divalent cation requirements for attachment to ECM molecules differed. For fibronectin substrata, trunk neural crest cells required divalent cations for attachment, whereas cranial neural crest cells bound in the absence of divalent cations. However, cranial neural crest cells lost this cation-independent attachment after a few days of culture. For laminin substrata, trunk cells used two integrins, one divalent cation-dependent and the other divalent cation-independent (Lallier, T. E. and Bronner-Fraser, M. (1991) Development 113, 1069–1081). In contrast, cranial neural crest cells attached to laminin using a single, divalent cation-dependent receptor system. Immunoprecipitations and immunoblots of surface labelled neural crest cells with HNK-1, α_1 integrin and β_1 integrin antibodies suggest that cranial and trunk neural crest cells possess biochemically distinct integrins. Our results demonstrate that cranial and trunk cells differ in their mechanisms of adhesion to selected ECM components, suggesting that they are non-overlapping populations of cells with regard to their adhesive properties

Tv-RIO1 – an atypical protein kinase from the parasitic nematode Trichostrongylus vitrinus

Background: Protein kinases are key enzymes that regulate a wide range of cellular processes, including cell-cycle progression, transcription, DNA replication and metabolic functions. These enzymes catalyse the transfer of phosphates to serine, threonine and tyrosine residues, thus playing functional roles in reversible protein phosphorylation. There are two main groups, namely eukaryotic protein kinases (ePKs) and atypical protein kinases (aPKs); RIO kinases belong to the latter group. While there is some information about RIO kinases and their roles in animals, nothing is known about them in parasites. This is the first study to characterise a RIO1 kinase from any parasite. Results: A full-length cDNA (Tv-rio-1) encoding a RIO1 protein kinase (Tv-RIO1) was isolated from the economically important parasitic nematode Trichostrongylus vitrinus (Order Strongylida). The uninterrupted open reading frame (ORF) of 1476 nucleotides encoded a protein of 491 amino acids, containing the characteristic RIO1 motif LVHADLSEYNTL. Tv-rio-1 was transcribed at the highest level in the third-stage larva (L3), and a higher level in adult females than in males. Comparison with homologues from other organisms showed that protein Tv-RIO1 had significant homology to related proteins from a range of metazoans and plants. Amino acid sequence identity was most pronounced in the ATP-binding motif, active site and metal binding loop. Phylogenetic analyses of selected amino acid sequence data revealed Tv-RIO1 to be most closely related to the proteins in the species of Caenorhabditis. A structural model of Tv-RIO1 was constructed and compared with the published crystal structure of RIO1 of Archaeoglobus fulgidus (Af-Rio1). Conclusion: This study provides the first insights into the RIO1 protein kinases of nematodes, and a foundation for further investigations into the biochemical and functional roles of this molecule in biological processes in parasitic nematodes

Black Extended Objects, Naked Singularities and P-Branes

We treat the horizons of charged, dilaton black extended objects as quantum mechanical objects. We show that the S matrix for such an object can be written in terms of a p-brane-like action. The requirements of unitarity of the S matrix and positivity of the p-brane tension equivalent severely restrict the number of space-time dimensions and the allowed values of the dilaton parameter a. Generally, black objects transform at the extremal limit into p-branes.Comment: 9 pages, REVTE