Dosimetric planning of radioiodine therapy on the basis of pharmacokinetic modeling

The program complex of pharmacokinetic modeling and dosimetric planning of radioiodine therapy on the basis of clinical diagnostic data is developed. For 16 patients with the diagnosis «diffuse toxic goiter» (Graves' disease) individual kinetic parameters of transport of the thyroid radiopharmaceutical taken orally are identified and calculations of the absorbed doses in the thyroid, the stomach, the blood tissue, and the periodic-depletion bladder are performed. Three approaches to purpose of activity of radiopharmaceutical and feature of individual dosimetric planning of radioiodine therapy are considered and analysed

The Tetraspanins CD9 and CD81 Regulate CD9P1-Induced Effects on Cell Migration

CD9P-1 is a cell surface protein with immunoglobulin domains and an unknown function that specifically associates with tetraspanins CD9 and CD81. Overexpression of CD9P-1 in HEK-293 cells induces dramatic changes in cell spreading and migration on various matrices. Experiments using time-lapse videomicroscopy revealed that CD9P-1 expression has led to higher cell motility on collagen I but lower motility on fibronectin through a β1-integrins dependent mechanism. On collagen I, the increase in cell motility induced by CD9P-1 expression was found to involve integrin α2β1 and CD9P-1 was observed to associate with this collagen receptor. The generation of CD9P-1 mutants demonstrated that the transmembrane and the cytoplasmic domains are necessary for inducing effects on cell motility. On the other hand, expression of tetraspanins CD9 or CD81 was shown to reverse the effects of CD9P-1 on cell motility on collagen I or fibronectin with a concomitant association with CD9P-1. Thus, the ratio of expression levels between CD9P-1 and its tetraspanin partners can regulate cell motility

Chemical Imaging on Liver Steatosis Using Synchrotron Infrared and ToF-SIMS Microspectroscopies

Fatty liver or steatosis is a frequent histopathological change. It is a precursor for steatohepatitis that may progress to cirrhosis and in some cases to hepatocellular carcinoma. In this study we addressed the in situ composition and distribution of biochemical compounds on tissue sections of steatotic liver using both synchrotron FTIR (Fourier transform infrared) and ToF-SIMS (time of flight secondary ion mass spectrometry) microspectroscopies. FTIR is a vibrational spectroscopy that allows investigating the global biochemical composition and ToF-SIMS lead to identify molecular species in particular lipids. Synchrotron FTIR microspectroscopy demonstrated that bands linked to lipid contribution such as -CH3 and -CH2 as well as esters were highly intense in steatotic vesicles. Moreover, a careful analysis of the -CH2 symmetric and anti-symmetric stretching modes revealed a slight downward shift in spectra recorded inside steatotic vesicles when compared to spectra recorded outside, suggesting a different lipid environment inside the steatotic vesicles. ToF-SIMS analysis of such steatotic vesicles disclosed a selective enrichment in cholesterol as well as in diacylglycerol (DAG) species carrying long alkyl chains. Indeed, DAG C36 species were selectively localized inside the steatotic vesicles whereas DAG C30 species were detected mostly outside. Furthermore, FTIR detected a signal corresponding to olefin (C = C, 3000-3060 cm−1) and revealed a selective localization of unsaturated lipids inside the steatotic vesicles. ToF-SIMS analysis definitely demonstrated that DAG species C30, C32, C34 and C36 carrying at least one unsaturated alkyl chain were selectively concentrated into the steatotic vesicles. On the other hand, investigations performed on the non-steatotic part of the fatty livers have revealed important changes when compared to the normal liver. Although the non-steatotic regions of fatty livers exhibited normal histological aspect, IR spectra demonstrated an increase in the lipid content and ToF-SIMS detected small lipid droplets corresponding most likely to the first steps of lipid accretion

The CD81 Partner EWI-2wint Inhibits Hepatitis C Virus Entry

Two to three percent of the world's population is chronically infected with hepatitis C virus (HCV) and thus at risk of developing liver cancer. Although precise mechanisms regulating HCV entry into hepatic cells are still unknown, several cell surface proteins have been identified as entry factors for this virus. Among these molecules, the tetraspanin CD81 is essential for HCV entry. Here, we have identified a partner of CD81, EWI-2wint, which is expressed in several cell lines but not in hepatocytes. Ectopic expression of EWI-2wint in a hepatoma cell line susceptible to HCV infection blocked viral entry by inhibiting the interaction between the HCV envelope glycoproteins and CD81. This finding suggests that, in addition to the presence of specific entry factors in the hepatocytes, the lack of a specific inhibitor can contribute to the hepatotropism of HCV. This is the first example of a pathogen gaining entry into host cells that lack a specific inhibitory factor

5. Le vecteur hydrogène

Si la production d’énergie subit une nouvelle révolution avec l’introduction massive des énergies renouvelables qui doivent supplanter progressivement les énergies fossiles, il en va de même pour les vecteurs énergétiques au sein desquels les vecteurs carbonés également d’origine fossile (charbon, carburants liquides, gaz naturel) devraient voir leurs usages diminuer. À ce titre, les biocarburants et l’électricité prennent une place grandissante, mais ils ne sont pas capables de répondre à l’..

Caractérisation des complexes à tétraspanines par des approches protéomiques

LE KREMLIN-B.- PARIS 11-BU Méd (940432101) / SudocSudocFranceF

CD9P-1 overexpression affects HEK-293 cell migration.

<p>HEK-293 or HEK/CD9P-1 cells were plated on various matrices. The same field of cells was monitored over a period of 24h while being maintained in a microscope stage incubator. Time-lapse videomicroscopy was performed at a rate of 1 frame per 15 min. Independent experiments were performed: laminin-5 (n = 3), matrigel (n = 3), collagen I (n = 20), fibronectin (n = 11); standard errors are shown.</p

Effects of tetraspanins on CD9P1-induced cell motility on fibronectin.

<p>HEK/CD9P-1 cells were transfected with tetraspanins CD9, CD81, CD82 or CD9×82. Then, cells were plated on fibronectin. Time-lapse videomicroscopy was performed at a rate of 1 frame per 15 min for 24h. The results correspond to three independent experiments; standard errors are shown. (*) p<0.05, (**) p<0.01.</p