15 research outputs found
BRCA1 methylation status alters protein-protein interactions at the 504-802 region.
<p>(<b>a</b>) Schematic of BRCA1 504-802 primary sequence depicting important protein-protein interactions and domains that could be affected by the methylation of this region. (<b>b</b>) MDA-MB-231 cells were treated with AdOx (30 µM) in order to observe BRCA1 methylation inhibition upon treatment. Two milligram of MDA-MB-231 whole cell protein extract was immunoprecipitated with anti-BRCA1 or anti-IgG antibodies, separated on a 4-20% gel by SDS-PAGE, and western blotted using antibodies against Sp1 and BRCA1 proteins. Input represents 1/10 of immunoprecipitated material. Results are representative of two independent experiments. (<b>c</b>) MDA-MB-231 cells were treated with AdOx (30 µM) and whole cell extract separated on a 4-20% gel by SDS-PAGE, and probed with anti-Sp1 antibody. Densitometry was averaged from three independent immunoblots.</p
Decreased levels of PRMT1 alters BRCA1 promoter binding <i>in vivo.</i>
<p>(<b>a</b>) HeLa cells were transfected with different concentrations of PRMT1 siRNA (10, 25, 50 nM) following manufacturer's instructions. Results are representative of two independent experiments. (<b>b</b>) HeLa cells transfected with 50 nM Luc or PRMT1 siRNA were collected for ChIP analysis. Anti-BRCA1 (10 µg), anti-IgG (10 µg), and anti-histone H3-phosphorylated at S10 (H3-pS10, 5 µg) antibodies were used for ChIP analysis. PCR products were run on a 2% agarose gel and visualized with ethidium bromide staining. Results are representative of two independent experiments.</p
Metabolite abundance ratios indicating up or down regulation for HTLV1 transformed T cells (C81, HUT102), and HTLV3 transfected and Tax1 or Tax3 expressing cells (293-HTLV3, H9-Tax1, 293-Tax3, respectively).
a<p>The metabolite numbers are according to the list provided in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012590#pone-0012590-t001" target="_blank">Table 1</a>.</p
Positive ion LAESI mass spectra of T lymphocytes.
<p>Peaks marked in A) non-HTLV1 transformed CEM T lymphocytes and B) HTLV1 transformed C81 T lymphocytes with an asterisk (*) indicate multiply charged ions of a peptide. Inset in panel A) shows the isotope pattern of the peak at m/z 993.5 with five charges.</p
Metabolites in the lipid metabolism pathway detected by LAESI-MS in T lymphocytes.
<p>Metabolites in the lipid metabolism pathway detected by LAESI-MS in T lymphocytes.</p
Lipid peaks detected in the LAESI spectra of non-HTLV1 transformed (CEM), and HTLV1 transformed (C81) T cells.
<p>Up and down regulation are followed by C81/CEM and CEM/C81 abundance ratios, respectively.</p><p>*PC  =  diacyl glycerophosphocholine, PC O-  =  alkylacyl or alkenylacyl glycerophosphocholine.</p
Tandem MS with collision induced dissociation for metabolite identification.
<p>A) protonated spermine (m/z 203.2), B) protonated glutathione (m/z 308.1) and C) protonated glycerophosphocholine lipid (PC(34:1), m/z 760.6) in CEM T lymphocytes, and of D) protonated adenosine monophosphate (AMP, m/z 348.1) in C81 T lymphocytes were directly analyzed by LAESI.</p
Expanded positive ion LAESI mass spectra showing glycerophosphocholine lipid peaks.
<p>Peaks marked with an asterisk (*) in A) CEM T lymphocytes B) HTLV1 transformed C81 T lymphocytes indicate multiply charged ions of a peptide.</p
Metabolites related to A) adenosine monophosphate (AMP) and B) dopamine detected by LAESI-MS in T lymphocytes.
<p>Metabolites related to A) adenosine monophosphate (AMP) and B) dopamine detected by LAESI-MS in T lymphocytes.</p
Metabolites in the creatine and polyamine biosynthesis pathways detected by LAESI-MS in T lymphocytes.
<p>Metabolites in the creatine and polyamine biosynthesis pathways detected by LAESI-MS in T lymphocytes.</p