Stimulation indices for the proliferative responses of human PBMC to recall stimulation with ffLVS for five days.

<p>Median values ± SEM per donor group of 12–16 individuals are shown. “pat” indicates convalescent tularemia patients, “vc” LVS vaccinees, and “nv” naïve individuals.</p

Histograms indicating the frequency of mono-, bi- and tri-functional cell subsets in CD45RO⁺ and CD45RA⁺ T-cell populations of convalescent patients.

<p>Mean values are illustrated throughout. The black axis indicates percentages for mono-functional IFN-γ-positive and all bi- and tri-functional T-cell subsets. The red axis indicates percentages for MIP-1β- and CD107a-positive mono-functional T cell subsets.</p

Histograms indicating the frequency of mono-, bi- and tri-functional cell subsets in CD45RO⁺ and CD45RA⁺ T-cell populations of vaccinees.

<p>Mean values are illustrated throughout. The black axis indicates percentages for mono-functional IFN-γ-positive and all bi- and tri-functional T-cell subsets. The red axis indicates percentages for MIP-1β- and CD107a-positive mono-functional T cell subsets.</p

Levels of cytokines secreted by human PBMC after recall stimulation with ffLVS or ffSchu S4 for five days.

<p>Cytokine concentrations were measured in cell culture supernatants using multiplex analysis. Median values ± SEM from PBMC samples of 14–16 individuals per donor group are shown (black bars indicate convalescent patients; grey bars indicate LVS vaccinees; white bars indicate naïve donors). Statistically significant differences between immune and naïve donors are marked by asterisks (<i>P</i><0.05). For IL-7, 36 out of 40 values were below the detection limit and therefore not included in the data analysis.</p

Heat map of Spearman correlation coefficients for iMFI values in various cell populations after intra-individual comparison for all donors.

<p>Integrated MFI values were obtained for all three functional markers (IFN-γ, MIP-1β, CD107a) in all mono-, bi- and trifunctional T-cell subsets and for all donors using <i>Clust</i> semi-automated gating. The iMFI values (24 values per PBMC sample, identified by numbers 1–24) from recall stimulation with 0.1 cfu ffLVS/PBMC were compared and lined up in a two-dimensional matrix. Correlated data are marked by shades of grey depending on the strength of the correlation coefficients; a coefficient above 0.7 is considered to indicate very strong correlation.</p

Histograms indicating the frequency of mono-, bi- and tri-functional cell subsets in CD45RO⁺ and CD45RA⁺ T-cell populations of non-vaccinated individuals.

<p>Mean values are illustrated throughout. The black axis indicates percentages for mono-functional IFN-γ-positive and all bi- and tri-functional T-cell subsets. The red axis indicates percentages for MIP-1β- and CD107a-positive mono-functional T cell subsets.</p

Levels of cytokines secreted by human PBMC after recall stimulation with ffLVS or ffSchu S4 for five days.

<p>Cytokine concentrations were measured in cell culture supernatants using multiplex analysis. Median values ± SEM from PBMC samples of 14–16 individuals per donor group are shown (black bars indicate convalescent patients; grey bars indicate LVS vaccinees; white bars indicate naïve donors). Statistically significant differences between immune and naïve donors are marked by asterisks (<i>P</i><0.05).</p