From fix to fit into the autoptic human brains.

Formalin-fixed, paraffinembedded (FFPE) human brain tissues are very often stored in formalin for long time. Formalin fixation reduces immunostaining, and the DNA/RNA extraction from FFPE brain tissue becomes suboptimal. At present, there are different protocols of fixation and several procedures and kits to extract DNA/RNA from paraffin embedding tissue, but a gold standard protocol remains distant. In this study, we analyzed four types of fixation systems and compared histo and immuno-staining. Based on our results, we propose a modified method of combined fixation in formalin and formic acid for the autoptic adult brain to obtain easy, fast, safe and efficient immunolabelling of long-stored FFPE tissue. In particular, we have achieved an improved preservation of cellular morphology and obtained success in postmortem immunostaining for NeuN. This nuclear antigen is an important marker for mapping neurons, for example, to evaluate the histopathology of temporal lobe epilepsy or to draw the topography of cardiorespiratory brainstem nuclei in sudden infant death syndrome (SIDS). However, NeuN staining is frequently faint or lost in postmortem human brain tissues. In addition, we attained Fluoro Jade C staining, a marker of neurodegeneration, and immunofluorescent staining for stem cell antigens in the postnatal human brain, utilizing custom fit fixation procedures

Productivity Dynamics across European Regions: the Impact of Structural and Cohesion Funds

This paper analyzes the impact of the European Union regional policy of the three programming periods 1975-1988, 1989-1993 and 1994-1999 on the dynamics of productivity of European regions. On average, funding had a positive, but concave, e\ufb00ect on productivity growth. In particular, a share of funds on GVA of 10% GVA is estimated to raise the regional growth rate of about 0.9% per year. However, by separately considering the three programming periods and the composition of the funds according to the objectives de\ufb01ned by the EU, we \ufb01nd that: i) only the funds allocated in the second and third programming periods, when they remarkably increased, had a signi\ufb01cant impact; and ii) only Objective 1 and Cohesion funds played a signi\ufb01cantly positive impact, while funds devoted to Objectives 2, 3, 4 and 5 had a negative or non signi\ufb01cant impact. The results are robust to potential endogeneity of funds and spatial dependence

Deep and Proximate Determinants of the World Income Distribution

This paper studies the deep and proximate determinants of the evolution of the cross-country distribution of GDP per worker in the period 1960–2008 by a novel method based on an information criterion. We find that countries of our sample follow three distinctive growth regimes identified by two deep determinants, namely life expectancy at birth in 1960 and the share of Catholics in 1965, and that each regime is characterized by non-linearities. Growth regimes appear to be the main cause of the increased inequality and polarization, while technological catch-up, proxied by the initial level of GDP per worker, acts in the opposite direction. Finally, human capital marginally reduces polarization, while investment rates and employment growth have no distributional effect

Counterfactual Distribution Dynamics across European Regions

This paper proposes a methodology which combines elements of parametric regression analysis with the nonparametric distribution dynamics approach in order to analyse the role of some variables in the convergence of productivity across European regions over the period 1980-2002. We find that the initial productivity crucially accounts in the convergence process across European regions. Differently, employment growth seems not to play a role, while the Structural and Cohesion Funds seem to play a positive role, even though such effect seems to be very low and statistically significant only at the low bound of the range of initial productivity. The structural change of regional economies plays a positive role, but such effect is statistically significant only for the least productive regions. The output composition of a region in 1980 affects the convergence process of productivity growth in several ways. In particular, the share of non market services on output acts like a source of convergence from 1980 to 2002 but in the long-run it plays a negligible role. Finally, the share of finance acts like a force of divergence across European regions, especially for the least productive regions

On the Determinants of Distribution Dynamics

n this paper we propose a novel approach to identify the impact of growth determinants on the distribution dynamics of productivit y. Our approach integrates counterfactual analysis with the estima tion of stochastic kernels. The counterfactuals are constructed from a semi-parametric growth regression, in which the cross-section heterogeneity in the growth determinants is removed. The methodology also allows us to test for potential distributional effects in the residuals. We illustrate the usefulness of the proposed methodology by an application to a cross-section of countries, which highlights the significant impact on inequality and polarization in the world productivity distribution of growth determinants from an augmented Solow model

On the Determinants of Distribution Dynamics

n this paper we propose a novel approach to identify the impact of growth determinants on the distribution dynamics of productivit y. Our approach integrates counterfactual analysis with the estima tion of stochastic kernels. The counterfactuals are constructed from a semi-parametric growth regression, in which the cross-section heterogeneity in the growth determinants is removed. The methodology also allows us to test for potential distributional effects in the residuals. We illustrate the usefulness of the proposed methodology by an application to a cross-section of countries, which highlights the significant impact on inequality and polarization in the world productivity distribution of growth determinants from an augmented Solow model

Neuroanatomical dysmorphology of the medial superior olivary nucleus in sudden fetal and infant death

doi: 10.3389/fnhum.2012.00322 Neuroanatomical dysmorphology of the medial superior olivary nucleus in sudden fetal and infant deat