26 research outputs found
Lipoic acid, but not tempol, preserves vascular compliance and decreases medial calcification in a model of elastocalcinosis
Blood Meal-Derived Heme Decreases ROS Levels in the Midgut of Aedes aegypti and Allows Proliferation of Intestinal Microbiota
The presence of bacteria in the midgut of mosquitoes antagonizes infectious agents, such as Dengue and Plasmodium, acting as a negative factor in the vectorial competence of the mosquito. Therefore, knowledge of the molecular mechanisms involved in the control of midgut microbiota could help in the development of new tools to reduce transmission. We hypothesized that toxic reactive oxygen species (ROS) generated by epithelial cells control bacterial growth in the midgut of Aedes aegypti, the vector of Yellow fever and Dengue viruses. We show that ROS are continuously present in the midgut of sugar-fed (SF) mosquitoes and a blood-meal immediately decreased ROS through a mechanism involving heme-mediated activation of PKC. This event occurred in parallel with an expansion of gut bacteria. Treatment of sugar-fed mosquitoes with increased concentrations of heme led to a dose dependent decrease in ROS levels and a consequent increase in midgut endogenous bacteria. In addition, gene silencing of dual oxidase (Duox) reduced ROS levels and also increased gut flora. Using a model of bacterial oral infection in the gut, we show that the absence of ROS resulted in decreased mosquito resistance to infection, increased midgut epithelial damage, transcriptional modulation of immune-related genes and mortality. As heme is a pro-oxidant molecule released in large amounts upon hemoglobin degradation, oxidative killing of bacteria in the gut would represent a burden to the insect, thereby creating an extra oxidative challenge to the mosquito. We propose that a controlled decrease in ROS levels in the midgut of Aedes aegypti is an adaptation to compensate for the ingestion of heme
Perfil dos usuários do Hiperdia de três unidades básicas de saúde do sul do Brasil Perfil de los usuarios registrados en el Hiperdia de tres unidades basicas de salud del Sur de Brasil Profile of users registered in Hiperdia three units of basic health South of Brazil
Objetivou-se descrever o perfil dos hipertensos e diabéticos cadastrados no sistema HiperDia do Ministério da Saúde em três unidades básicas do município de Pelotas, na região Sul do Rio Grande do Sul. Trata-se de um estudo transversal com abordagem quantitativa. Utilizou-se como instrumento de pesquisa um formulário baseado na ficha de cadastro do sistema HiperDia, que fornece informações importantes sobre a população atendida. Os dados coletados foram digitados no programa Epi-info 6.04 e analisados no software STATA 9.0. A população avaliada no estudo é predominantemente feminina, na faixa etária acima dos 50 anos. Os fatores de risco mais presentes foram o sobrepeso, o hábito de vida sedentário, a hereditariedade e como complicações crônicas as cardiovasculares. Concluiu-se que o perfil dos cadastrados é semelhante ao encontrado no banco de dados nacional do SisHiperDia.<br>El objetivo fue describir el perfil de pacientes hipertensos y diabéticos inscritos en el sistema HIPERDIA del Ministerio de la Salud en tres unidades básicas de Pelotas en el sur de Rio Grande do Sul, Brasil. Estudio transversal con abordaje cuantitativo; se utilizó como herramienta de investigación un formulario basado en el registro del sistema HIPERDIA, este sistema proporciona informaciones importantes sobre la población atendida. Los datos recolectados fueron ingresados en el programa Epi-Info 6,04 y analizados en STATA 9.0. La población evaluada es predominantemente femenina, del grupo etario mayor de 50 años. Los factores de riesgo más presentes fueron sobrepeso, estilo de vida sedentario, herencia y complicaciones crónicas cardiovasculares. Se concluyó que el perfil de inscritos es similar a lo encontrado en la base de datos nacional de SisHiperDia.<br>The objective was to describe the profile of hypertensive and diabetic patients registered in the HiperDia system of the Ministry of Health in three basic units of Pelotas in southern Rio Grande do Sul, Brazil. This is a transversal study with quantitative approach. The research tool used was a form based in the registration form of the HiperDia system, this system provides important information about the served population. The collected data were entered in Epi-Info 6.04 program and analyzed in STATA 9.0 software. The population evaluated in the study is predominantly female, aged above 50 years. The more risk factors present were overweight, sedentary lifestyle habits, heredity and chronic cardiovascular complications. We concluded that the profile of the registered patients is similar to that found in the national database of SisHiperDia