Alcohol in the life narratives of women: Commonalities and differences by sexual orientation

Aim: The aim of this study was to explore social representations of alcohol use among women, with a focus on possible differences between sexual minority and heterosexual women. Methods: This qualitative study was part of a larger study examining mediators of heavier drinking among sexual minority women (lesbian identified, bisexual identified, and heterosexual identified with same sex partners) compared to heterosexual women based on the National Alcohol Survey. Qualitative in-depth life history interviews were conducted over the telephone with 48 women who had participated in the 2009–2010 National Alcohol Survey, including respondents representing different sexual orientation groups. Questions explored the lives and experiences of women, and how use of alcohol may connect to those experiences. Findings: Representations about normative and risky use included social use, use in relation to community connection, addictive use and self-medication. Other representations common across groups included articulation of boundaries that were defined by negative exemplars, marked by indicators of loss of control, and maintained through selective engagement of social networks. Although representations across groups were similar, some representations, such as alcohol use in fostering community connection, appeared to be more salient for sexual minority women. The findings of the study underscore the importance of considering both commonalities and potential differences among women by sexual orientation in meanings and perceived risks associated with alcohol use in future research and intervention efforts

Sexual Minority Women and Alcohol: Intersections between drinking, relational contexts, stress, and coping

Few studies explore sexual-minority women\u27s experiences and perceptions of alcohol. Qualitative interviews were conducted with six sexual-minority women who reported having sought help for alcohol problems in the past and six who did not. Themes emerged in two broad areas: (1) stressors that contributed to heavy or problem drinking and (2) factors that enhanced coping and reduced both stress and problem use. Alcohol use across groups was framed in terms of social context (e.g., bar patronage), stress management, and addiction. The findings of the study underscore the importance of considering the role of alcohol in managing stress as well as coping factors that may inform social service interventions

Roles of Predicted Glycosyltransferases in the Biosynthesis of the Rhizobium etli CE3 O Antigen

The Rhizobium etli CE3 O antigen is a fixed-length heteropolymer. The genetic regions required for its synthesis have been identified, and the nucleotide sequences are known. The structure of the O antigen has been determined, but the roles of specific genes in synthesizing this structure are relatively unclear. Within the known O-antigen genetic clusters of this strain, nine open reading frames (ORFs) were found to contain a conserved glycosyltransferase domain. Each ORF was mutated, and the resulting mutant lipopolysaccharide (LPS) was analyzed. Tricine SDS-PAGE revealed stepwise truncations of the O antigen that were consistent with differences in mutant LPS sugar compositions and reactivity with O-antigen-specific monoclonal antibodies. Based on these results and current theories of O-antigen synthesis, specific roles were deduced for each of the nine glycosyltransferases, and a model for biosynthesis of the R. etli CE3 O antigen was proposed. In this model, O-antigen biosynthesis is initiated with the addition of N-acetyl-quinovosamine-phosphate (QuiNAc-P) to bactoprenol-phosphate by glycosyltransferase WreU. Glycosyltransferases WreG, WreE, WreS, and WreT would each act once to attach mannose, fucose, a second fucose, and 3-O-methyl-6-deoxytalose (3OMe6dTal), respectively. WreH would then catalyze the addition of methyl glucuronate (MeGlcA) to complete the first instance of the O-antigen repeat unit. Four subsequent repeats of this unit composed of fucose, 3OMe6dTal, and MeGlcA would be assembled by a cycle of reactions catalyzed by two additional glycosyltransferases, WreM and WreL, along with WreH. Finally, the O antigen would be capped by attachment of di- or tri-O-methylated fucose as catalyzed by glycosyltransferase WreB

Lines in the sand: Social representations of substance use boundaries in life narratives

This study identifies social representations in interviews about alcohol and substance use in the discourse of 129 young adults, who were interviewed for 2.5 to 3.5 hr each for their life histories and use or nonuse of alcoholic beverages and drugs. Respondents spontaneously delineated their substance use boundaries, creating a continuum of behaviors with boundary points separating acceptable from unacceptable behaviors. They used signaling expressions to indicate go and stop signs and movement along the substance use continuum and reported negotiating substance use boundaries both internally and with peers. A ubiquitous narrative element was the cautionary tale, in which a negative exemplar goes too far with alcohol and/or drugs, providing an example of the possible negative outcomes of transgressing boundaries. In general, the narratives revealed complex relationships to alcohol and other drugs that may be useful in refining messages for more effective communication in prevention and intervention programs

Getting on the E List: E-Mail Use in a Community of Service Provider

This case examines how a community of organizations providing service to people experiencing homelessness made use of an electronic mail list. Current economic conditions have encouraged organizations in various sectors—including nonprofits—that might normally compete for scarce resources to collaborate with one another to increase their chances of survival. One set of tools likely to be of value in such relationships includes various online discussion technologies. An examination of this community’s email list use over a three-year period suggests a somewhat complex picture regarding technology use. More specifically, some issues both constrain and enable use. Additionally, seemingly basic and minimal uses of the list provided not only the greatest functionality for the users, but also led to several unanticipated consequences for those involved

\u3cem\u3eIn Vitro\u3c/em\u3e Biosynthesis and Chemical Identification of UDP-\u3cem\u3eN\u3c/em\u3e-acetyl-d-quinovosamine (UDP-d-QuiNAc)

N-acetyl-d-quinovosamine (2-acetamido-2,6-dideoxy-d-glucose, QuiNAc) occurs in the polysaccharide structures of many Gram-negative bacteria. In the biosynthesis of QuiNAc-containing polysaccharides, UDP-QuiNAc is the hypothetical donor of the QuiNAc residue. Biosynthesis of UDP-QuiNAc has been proposed to occur by 4,6-dehydration of UDP-N-acetyl-d-glucosamine (UDP-GlcNAc) to UDP-2-acetamido-2,6-dideoxy-d-xylo-4-hexulose followed by reduction of this 4-keto intermediate to UDP-QuiNAc. Several specific dehydratases are known to catalyze the first proposed step. A specific reductase for the last step has not been demonstrated in vitro, but previous mutant analysis suggested that Rhizobium etli gene wreQ might encode this reductase. Therefore, this gene was cloned and expressed in Escherichia coli, and the resulting His6-tagged WreQ protein was purified. It was tested for 4-reductase activity by adding it and NAD(P)H to reaction mixtures in which 4,6-dehydratase WbpM had acted on the precursor substrate UDP-GlcNAc. Thin layer chromatography of the nucleotide sugars in the mixture at various stages of the reaction showed that WbpM converted UDP-GlcNAc completely to what was shown to be its 4-keto-6-deoxy derivative by NMR and that addition of WreQ and NADH led to formation of a third compound. Combined gas chromatography-mass spectrometry analysis of acid hydrolysates of the final reaction mixture showed that a quinovosamine moiety had been synthesized after WreQ addition. The two-step reaction progress also was monitored in real time by NMR. The final UDP-sugar product after WreQ addition was purified and determined to be UDP-d-QuiNAc by one-dimensional and two-dimensional NMR experiments. These results confirmed that WreQ has UDP-2-acetamido-2,6-dideoxy-d-xylo-4-hexulose 4-reductase activity, completing a pathway for UDP-d-QuiNAc synthesis in vitro

Designing Connection

Social connection is an essential human need. Personal connections exist at a variety of depths and within different types of relationships. Small daily choices determine the strength of those connections and their impact on our well-being as individuals and as a community. Modern society and technology have altered the speed and channels of connection, increasing communication but decreasing meaningful connection. It is critical to understand how the methods of communication affect the depth of connections. Through a series of participatory graphic design projects, I analyze the strengths and weaknesses of one-on-one, small group and community connections within an established taxonomy of the levels of connection, to identify the factors that contribute to strong social relationships

Advantages of Looping at the Elementary Level

Advantages of Looping at the Elementary Level Recently, looping has been getting attention from educators and educational reformers as a low cost, easily implemented way to improve education. In this project, the author created an inservice presentation designed as an introduction to the practice of looping at the elementary level. The target audience was elementary teachers and administrators. The presentation provided a brief history of the practice of looping, the theory behind the practice, a synthesis of the benefits and possible challenges of looping, and finally, several tips and considerations for the implementation of looping

The Instructional Perspectives of Community College Mathematics Faculty

This study investigated the beliefs, feelings, and behaviors of full-time mathematics faculty at community colleges in a Midwestern state. The online questionnaire for this study included the modified Instructional Perspectives Inventory [IPI] (Henschke, 1989; Stanton, 2005). The subscales of the IPI are: (1) Teacher empathy with learners; (2) Teacher trust of learners; (3) Planning and delivery of instruction; (4) Accommodating learner uniqueness; (5) Teacher insensitivity toward learners; (6) Experience-based learning techniques; and, (7) Teacher-centered learning processes. Approximately 23.4% of invited participants responded to the survey, yielding a sample size of 34. Statistical analyses included calculations of mean, standard deviation, and standard error for summative subscale scores and summative overall IPI scores. Using a rankings scale proposed by Stanton (2005) [ Low below average , below average , average , above average , high above average ], all groups for this study were found to be average or below average in the application of andragogical / adult education principles. Analyses of Variance (ANOVA\u27s) revealed statistically significant differences for subscales one, two, four, five, and for summative overall IPI scores. Using a reliability rating scale suggested by George and Mallery (as cited in Gliem & Gliem, 2003, p. 87), subscales one through six were interpreted as having good or acceptable internal consistency. Subscale seven was found to have questionable consistency for this population. Recommendations for future research with the IPI include a consideration of the influence of gender, a calculation and interpretation of Cronbach\u27s alpha reliability coefficient and the Spearman-Brown prophecy coefficient, and the inclusion of a qualitative research component