13 research outputs found
Imaging Redox Activity at Bipolar Electrodes by Indirect Fluorescence Modulation
Bipolar electrochemistry (BPE) is
nowadays well-known but relatively
underexploited and still considered as unconventional. It has been
used, among others, in the frame of materials science and most importantly
has also found very promising applications in analytical chemistry.
Here, we extend this emerging field of analytical applications to
the development of a new sensing concept based on indirect BPE. This
approach is based on the generation of local pH gradients which will
allow detecting indirectly redox-active molecules due to a modulation
of the fluorescence intensity in the vicinity of a bipolar electrode
Indirect Bipolar Electrodeposition
Based on the principles of bipolar electrochemistry,
localized
pH gradients are generated at the surface of conducting particles
in solution. This allows the toposelective deposition of inorganic
and organic polymer layers via a pH-triggered precipitation mechanism.
Due to the intrinsic symmetry breaking of the process, the concept
can be used to generate in a straightforward way Janus particles,
with one section consisting of deposits obtained from non-electroactive
precursors. These indirect electrodeposits, such as SiO<sub>2</sub>, TiO<sub>2</sub>, or electrophoretic paints, can be further used
as an immobilization matrix for other species like dyes or nanoparticles,
thus opening promising perspectives for the synthesis of a variety
of bifunctional objects with a controlled shape
Bipolar Electrode Array Embedded in a Polymer Light-Emitting Electrochemical Cell
A linear
array of aluminum discs is deposited between the driving electrodes
of an extremely large planar polymer light-emitting electrochemical
cell (PLEC). The planar PLEC is then operated at a constant bias voltage
of 100 V. This promotes <i>in situ</i> electrochemical doping
of the luminescent polymer from both the driving electrodes and the
aluminum discs. These aluminum discs function as discrete bipolar
electrodes (BPEs) that can drive redox reactions at their extremities.
Time-lapse fluorescence imaging reveals that p- and n-doping that
originated from neighboring BPEs can interact to form multiple light-emitting
p–n junctions in series. This provides direct evidence of the
working principle of bulk homojunction PLECs. The propagation of p-doping
is faster from the BPEs than from the positive driving electrode due
to electric field enhancement at the extremities of BPEs. The effect
of field enhancement and the fact that the doping fronts only need
to travel the distance between the neighboring BPEs to form a light-emitting
junction greatly reduce the response time for electroluminescence
in the region containing the BPE array. The near simultaneous formation
of multiple light-emitting p–n junctions in series causes a
measurable increase in cell current. This indicates that the region
containing a BPE is much more conductive than the rest of the planar
cell despite the latter’s greater width. The p- and n-doping
originating from the BPEs is initially highly confined. Significant
expansion and divergence of doping occurred when the region containing
the BPE array became more conductive. The shape and direction of expanded
doping strongly suggest that the multiple light-emitting p–n
junctions, formed between and connected by the array of metal BPEs,
have functioned as a single rod-shaped BPE. This represents a new
type of BPE that is formed <i>in situ</i> and as a combination
of metal, doped polymers, and forward-biased p–n junctions
connected in series
Electropolymerization of Polypyrrole by Bipolar Electrochemistry in an Ionic Liquid
Bipolar electrochemistry has been
recently explored for the modification
of conducting micro- and nanoobjects with various surface layers.
So far, it has been assumed that such processes should be carried
out in low-conductivity electrolytes in order to be efficient. We
report here the first bipolar electrochemistry experiment carried
out in an ionic liquid, which by definition shows a relatively high
conductivity. Pyrrole has been electropolymerized on a bipolar electrode,
either in ionic liquid or in acetonitrile. The resulting polymer films
were characterized by scanning electron microscopy and by contact
profilometry. We demonstrate that the films obtained in an ionic liquid
are thinner and smoother than the films synthesized in acetonitrile.
Furthermore, a well-defined band of polypyrrole can be obtained in
ionic liquid, in contrast to acetonitrile for which the polypyrrole
film is present on the whole anodic part of the bipolar electrode
Comprehensive Study of DNA Binding on Iron(II,III) Oxide Nanoparticles with a Positively Charged Polyamine Three-Dimensional Coating
Iron (II,III) oxide Fe<sub>3</sub>O<sub>4</sub> nanoparticles (25
and 50 nm NPs) are grafted with amine groups through silanization
in order to generate a positively charged coating for binding negatively
charged species including DNA molecules. The spatial nature of the
coating changes from a 2-D-functionalized surface (monoamines) through
a layer of amine oligomers (diethylenetriamine or DETA, about 1 nm
in length) to a 3-D layer of polyamine (polyethyleneimine or PEI,
thickness ≥3.5 nm). These Fe<sub>3</sub>O<sub>4</sub>–PEI
NPs were prepared by binding short-chain PEI polymers to the iodopropyl
groups grafted on the NP surface. In this work, the surface charge
density, or zeta potential, of the nanoparticles is found not to be
the only factor influencing the DNA binding capacity, which also seems
not to be affected by their buffering capacity profile in the range
of pH 4–10. This study also allows the investigation of this
3-D effect on the surface of a nanoparticle as opposed to conventional
2-D amine functionalization. The flexibility of the PEI coating, which
consists of only 1, 2, and 3° amines, on the nanoparticle surface
has a significant influence on the overall DNA binding capacity and
the binding efficiency (or N/P ratio). These polyamine-functionalized
nanoparticles can be used in the purification of biomolecules and
the delivery of drugs and large biomolecules
Additional file 1: Figure S1. of Performance of genomic prediction within and across generations in maritime pine
Scatter plots (lower diagonal), histograms (diagonal) and correlations, with their significance (H0: r = 0 , upper diagonal), between breeding values for the traits: circumference, height and stem straightness. Individuals from the G0 generation are in blue, G1 in orange and G2 in green. Figure S2. P. pinaster composite map [40]. Markers in red correspond to 3965 of the 4335 SNPs used for genomic prediction analysis. Figure S3. Pedigree of the 818 trees comprising the reference population (NS = 25) with the following frequency for each generation: G0 = 46, G1 = 62 and G2 = 710. Links in purple represent mother–progeny relationships and those in orange represent father–progeny relationships. Pedigree Viewer software was used to represent the relatedness between individuals from the three generations. Figure S4. Pairwise linkage disequilibrium (LD) based on 3962 single-nucleotide polymorphisms mapped onto the twelve linkage groups (LG) of P. pinaster. Only loci with minor allele frequencies greater than 0.01 were included in the analysis. Figure S5. Distribution of the fixation index (FST) over the 12 chromosome of maritime pine. The top panel represents the FST between G0 and G1 and the bottom panel represents the FST between G1 and G2. Figure S6. Comparison of prediction accuracies across three sampling and two calibration strategies. Three sampling strategies for the selection of 20 % of the G2 population for use as the validation set were used: random, S1: between half-sib families and S2: within full-sib families. Two calibration scenarios were used for each sampling strategy. For predictions for the 20 % of the G2 population selected, we used the remaining 80 % of the G2 (in green) plus their progenitors (G0 and G1, in blue) as the calibration set. The results for models based on pedigree information (ABLUP) and marker information (GBLUP and B-LASSO), and the results for the three traits studied (tree diameter, height and stem straightness) are presented. The data are represented in a Tukey boxplot. Table S1. Prediction accuracy and status number (NS) for four methods of selecting G2 individuals. Prediction accuracy was estimated with three relationship matrices (AP, AF and G). Mean values (standard deviation in parentheses) are based on 100 replicates per model. The four selection methods were: Random, HS: half-sib family, FS: full-sib family and CD: coefficient of determination. Table S2. Prediction accuracy and bias for the use of the progeny population for validation (calibration set = G0 and G1, validation set = G2). The results for the ABLUP, GBLUP and Bayesian LASSO (B-LASSO) models and for the traits tree circumference, height and stem straightness are presented. (PDF 1725 kb
Bright Electrochemiluminescence Tunable in the Near-Infrared of Chiral Cationic Helicene Chromophores
Cationic
helicenes are <i>ortho</i>-fused polyaromatics
which exhibit well-defined and stable helical conformations with original
absorption and emission properties in the red to near-infrared spectral
ranges. Herein, a selection of cationic [4] and [6]helicenes are studied
for their electrochemical, fluorescence, and electrochemiluminescence
(ECL) properties in acetonitrile solutions. Their photophysical and
redox responses are drastically tuned by the introduction of auxochrome
substituents at their periphery or the interconversion of oxygen and
nitrogen atoms within the helical core. All diaza helicenes exhibit
a reversible reduction process, whereas in the presence of oxygen
instead of nitrogen atoms in the helical core, irreversible oxidations
and a decrease of ECL intensity are observed. ECL emission was successfully
produced with two sacrificial coreactants (benzoyl peroxide and tri-<i>n</i>-propylamine, TPrA). [4]Helicene <b>DMQA</b><sup><b>+</b></sup>, [6]helicene <b>DIAZA(Pr/Br)</b><sup><b>+</b></sup>, and <b>DIAZA(Hex/Br)</b><sup><b>+</b></sup> exhibit similar ECL emission wavelength in the near-infrared
region and generate very intense ECL signals. Their ECL efficiencies
are up to 2.6 times higher than that of the reference compound [Ru(bpy)<sub>3</sub>]<sup>2+</sup> when using TPrA as coreactant. A thermodynamic
map gathering both oxidation and reduction potentials and fluorescence
data is proposed for the prediction of energy sufficiency needed in
both coreactant ECL systems. Such a systematic overview based on the
photophysical and electrochemical properties may guide the conception
and synthesis of new chromophores with a strong ECL proficiency
Eosin-Mediated Alkylsulfonyl Cyanation of Olefins
Eosin-Y (EY)-mediated
alkylsulfonyl cyanation of olefins was shown
to afford alkylsulfonyl nitriles in good yields. On the basis of transient
absorption spectroscopy, the reaction was shown to proceed via photoinduced
electron transfer from <sup>3</sup>EY* to an O-cyanated derivative
of the photocatalyst, formed in situ, with generation of the corresponding
sulfinate that is oxidized by EY<sup>•.+</sup> into a sulfonyl
radical. Addition of the latter on the olefin, followed by a radical
cyano group transfer, then furnished the nitrile along with a RSO<sub>2</sub> radical sustaining the radical chain
Significant (<i>p</i><0.001) marker-trait pairs and their location on the genetic map, for height (HT) and stem straightness (STR).
<p>Significant (<i>p</i><0.001) marker-trait pairs and their location on the genetic map, for height (HT) and stem straightness (STR).</p
Descriptive statistics for the genetic linkage map of the parental genotype (H12 hybrid) of the F2 mapping population.
<p>Descriptive statistics for the genetic linkage map of the parental genotype (H12 hybrid) of the F2 mapping population.</p