Perbandingan Abbott Real-time High-risk HPV dan Cobas 4800 HPV Test untuk Deteksi Molekuler HPV pada Sampel Indonesia

Based on the results of 2013 National Basic Health Research (Riskesdas 2013), as many as 10.3% of female death due to cancer in Indonesia caused by cervical cancer of Human Papillomavirus (HPV) became the main risk factor that was 99.7%. The incidence of cervical cancer can be prevented through early detection of HPV infection moleculary. This study aims to evaluate the ability of Abbot Real-time High-risk HPV (AR) and Roche Cobas HPV (CB) techniques to detect HPV DNA from Indonesia‘s Stored Biology. Furthermore, the results from both techniques are confirmed by using genotyping method using Linnear Array (LA). A total of 74 speciment of stored biological materials (BBT) of cervical swabs stored in Phosphate Buffered Saline (PBS) media were extracted using a special isolation kit from Abbott Real-time High-risk HPV, Cobas 4800 Human Papillomavirus Test and Linear Array. Concentration and purity of HPV viral DNA extraction results were measured by spectophotometry method. The amplification of the extraction results was carried by using a specific primer segment of the L1 gene as a target for detecting HPV DNA. The quality of HPV DNA extraction results using AR technique was 10-30 ng/μL concentration in average, the result was higher when compared to the CB technique which only range from 10-20 ng/μL, but the results using the LA technique had a concentration ≥ 30 ng/μL in average. The purity of HPV DNA extraction using AR and CB technique was similar more or less. The examination of 74 samples had found HPV 16, HPV 18, and other HPV type in each technique, which were 14 positive (9, 4, and 1) in AR technique, 5 positive (3,2, and 0) in CB technique, and 14 positive (8, 4, and 2) in LA technique. AR technique is more accurate and optimal than CB technique in detecting HPV virus and identifying HPV virus types. Both AR and CB technique have their own advantages and disadvantages, although they have uses an automated system of isolation and readout results analysis. It could be concluded that AR technique has better performance in detecting HPV when compared to CB technique