Alternative splicing of exon 30 of <i>PKD1</i>.

<p>Four patients with polycystic kidney disease but normal kidney function (lane 1, 3, 5, 7) and four relatives with end stage renal disease (Lane 2, 4, 6 and 8) were tested. Lane 9 is positive control, lane 10 negative control of PCR. The non spliced fragment is 300 base pairs and the spliced fragment is 173 bases pairs (bp).</p

List of genes identified alternatively spliced in fibroblasts by microarrays.

<p>List of genes identified alternatively spliced in fibroblasts by microarrays.</p

Clinical and biological characteristics of the three groups.

<p>For numeric values, data were expressed in median, CI 95%. For clinical values, data were expressed in number (%).</p

PCR of <i>ADH1B</i> to explore the alternative splicing of exon 1 in PBMC.

<p>P1, P2, and P3 represent PCR product of three different PBMC from HD patient, C2 represent fibroblast in normal condition. Lane 1 is the PCR for the transcript 1-2-3 and lane 2 is for the transcript 2-3. There is no expression of ADH1B in PBMC (P1, P2, P3) but the gene is expressed in fibroblast (C2).</p

PCR <i>ADH1B</i> to explore the aternative splicing of exon 1 in fibroblasts.

<p>In normal serum, (C1), the two forms of the transcript is present, lane 4 transcript with exon 1-2-3 (983pb) and lane 5, transcript with exon 2 and 3 (454pb). In uremic serum (U3),the transcript included exon 1 is not present; lane 1 : no transcript, lane 2, transcript with exon 2 and 3.</p

Genes involved in lipid homeostasis based on microarray analyses.

<p>Genes involved in lipid homeostasis based on microarray analyses.</p

Biochemical parameters from the different groups (*p < 0.05).

<p>Biochemical parameters from the different groups (*p < 0.05).</p

Genes involved in the metabolism of xenobiotics based on microarray analyses.

<p>Genes involved in the metabolism of xenobiotics based on microarray analyses.</p

Real-time RT-PCR validation of genes selected within the muscles after microarray analyses (*p < 0.05).

<p>Real-time RT-PCR validation of genes selected within the muscles after microarray analyses (*p < 0.05).</p

Primers used for real-time qRT-PCR experiments.

<p>Primers used for real-time qRT-PCR experiments.</p