Bacterial loads in the skin at the inoculation site was measured by quantitative PCR for the <i>B</i>. <i>burgdorferi flaB</i> gene and normalized to copies of mouse <i>gapdh</i>.

<p>Values between strains were not statistically different (two-way ANOVA test using the Sidak-Bonferroni method). # values close to the detection limit.</p

Bacterial loads in the skin.

<p>The spirochetal burden in the skin at the inoculation site was measured by quantitative PCR for the <i>B</i>. <i>burgdorferi flaB</i> gene and normalized to copies of mouse <i>gapdh</i>. Values represent relative expression + SD of three independent experiments. Values between strains were not statistically different (two-way ANOVA test using the Sidak-Bonferroni method). (d: days).</p

Inflammatory profiles of <i>B</i>. <i>burgdorferi</i> ss, parental strain and its clone 297/4.

<p>Levels of transcripts were measured by RT-qPCR from the skin at the inoculation site. The values were calculated using the 2-delta delta Ct method after normalization with <i>gapdh</i>. (h: hours, d: days). Two-way ANOVA was used to analyze the data. At least, three mice were analyzed for each time point.</p

Bacterial loads in the skin at the inoculation site was measured by quantitative PCR for the <i>B</i>. <i>burgdorferi flaB</i> gene and normalized to copies of mouse <i>gapdh</i>.

<p>Values between strains were not statistically different (two-way ANOVA test using the Sidak-Bonferroni method). # values close to the detection limit.</p

Proteomic analyses of <i>B</i>. <i>burgdorferi</i> ss, 297 parental strain and its clone 297/4.

<p>(A) The Venn diagram shows the protein overlap and the proteins specifically identified in the parental strain and the clone 297/4. (B) Graphic representation of the breakdown of the proteins specifically identified in the clone. Categorization was based upon JCVI annotation. The percentages represent the fraction of that category within the proteins. (C) Gene location of the specific proteins identified in the clone 297/4.</p

Inflammatory profiles of the different strains of <i>B</i>. <i>burgdorferi</i> ss.

<p>Levels of transcripts were measured by RT-qPCR from the skin at the inoculation site. The values were calculated using the 2-delta delta Ct method after normalization with <i>gapdh</i>. (h: hours, d: days). Two-way ANOVA was used to analyze the data. At least three mice were analyzed for each time point.</p