miR-135b overexpression increases cell migration and invasion in MET1, PM1 and MET4 cSCC cell lines.

<p>A, B) miR-135b mimic significantly increases cSCC cell migration in the wound scratch assay within 24 hours post-transfection. C, D) miR-135b mimic significantly increases cSCC cell invasion in the Matrigel transwell invasion assay in PM1 cell line. * indicate p values less than 0.01. A) <i>Bar = 100um</i>; B) <i>Bar = 200um</i>.</p

Statistical analysis of differentially expressed miRNAs in cSCC tissues from organ transplant recipients.

<p>Statistical analysis of differentially expressed miRNAs in cSCC tissues from organ transplant recipients.</p

Functional assays of miR-135b inhibition and overexpression.

<p>A) Baseline mRNA miR-135b expression in 3 different OTR-derived cSCC cell lines was established by qRT-PCR. B) Effect of miR-135b inhibition on LZTS1 mRNA expression in cSCC lines by qRT-PCR within 48 hours post-transfection. C) Effect of miR-135b overexpression on LZTS1 mRNA expression in cSCC lines by qRT-PCR within 48 hours post-transfection. * indicate p values less than 0.005.</p

Immunohistochemical analysis of LZTS1 protein levels in normal human skin and cSCC tissues.

<p>A, C) LZTS1 staining is localized in the normal epidermis, but is largely absent in the tumor tissue. Arrows point towards the tumor nests. The absence of staining with control rabbit IgG antibody confirms the specificity of LZTS1 antibody. B, D) Evaluation of LZTS1 staining intensity shows significant reduction of LZTS1 staining in the tumor tissue when compared to normal non-lesional epidermis. * indicate p values less than 0.001. <i>Bar = 100um</i>.</p

Statistical analysis of differentially expressed miRNAs in cSCC tissues from immunocompetent patients.

<p>Statistical analysis of differentially expressed miRNAs in cSCC tissues from immunocompetent patients.</p

Differentially expressed miRNAs in human cSCC tissue.

<p>A) PCR array analysis of miRNA expression in cSCC tumors from immunocompetent patients (n = 32). B) PCR array analysis miRNA expression in cSCC tumors from organ transplant recipients (n = 11).</p